Abstract:
:To understand the role of nitric oxide (NO) in controlling the specific DNA-binding activities of transcriptional factors, we investigated the in vitro effect of the NO-donor sodium nitroprusside (SNP) on the AP-1 activity of cultured mouse cerebellar granule cells. A gel-mobility assay showed that SNP inhibited AP-1 activity in the presence, but not the absence of dithiothreitol (DTT). This DTT-dependent inhibition of AP-1 activity by SNP corresponded with the activation of the chemical reactivity of SNP with DTT, which can be monitored by the production of nitrite (NO2-). In contrast, diamide, a typical sulfhydryl oxidizing agent, inhibited AP-1 activity in the absence of DTT and its inhibitory effect was reversed competitively by DTT. Studies using structurally or functionally related analogues of SNP demonstrated that S-nitrosylation of the AP-1 moiety mediated by some NO-carriers but not by free NO, which can be produced by the chemical reaction of SNP with DTT, was responsible for the inhibition of AP-1 activity, suggesting NO-mediated regulation of the AP-1 transcriptional factor.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tabuchi A,Sano K,Oh E,Tsuchiya T,Tsuda Mdoi
10.1016/0014-5793(94)00839-6subject
Has Abstractpub_date
1994-08-29 00:00:00pages
123-7issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)00839-6journal_volume
351pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00534-g
更新日期:1995-06-19 00:00:00
abstract::Several peptidic and non-peptidic factors can modulate Na,K-ATPase activity, among them mainly inhibitors of this enzyme, ouabain being the most effective. In a very few cases only, activation of Na,K-ATPase by endogenous factors has been recorded. We have investigated the effect on Na,K-ATPase of a novel regulatory p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00407-2
更新日期:1994-05-23 00:00:00
abstract::Wild-type trypanosomal triosephosphate isomerase (wtTIM) is a very tight dimer. The interface residue His-47 of wtTIM has been mutated into an asparagine. Ultracentrifugation data show that this variant (H47N) only dimerises at protein concentrations above 3 mg/ml. H47N has been characterised at a protein concentratio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00586-x
更新日期:1995-07-03 00:00:00
abstract::In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81331-8
更新日期:1982-12-13 00:00:00
abstract::Glucosamine-6-phosphate (GlcN6P) N-acetyltransferase 1 (GNA1) is a key enzyme in the pathway toward biosynthesis of UDP-N-acetylglucosamine, an important donor substrate for N-linked glycosylation. GNA1 catalyzes the formation of N-acetylglucosamine-6-phosphate (GlcNAc6P) from acetyl-CoA (AcCoA) and the acceptor subst...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.07.040
更新日期:2008-09-03 00:00:00
abstract::The effect of caffeine on catecholamine secretion and intracellular free Ca2+ concentration [( Ca2+]i) in bovine adrenal chromaffin cells was examined using single fura-2-loaded cells and cell populations. In cell populations caffeine elicited a large (approximately 200 nM) transient rise in [Ca2+]i that was independe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81514-o
更新日期:1990-06-18 00:00:00
abstract::We have measured SGLT1 mRNA content and SGLT1-mediated glucose transport at different positions along the small intestine of control and streptozotocin diabetic rats and shown both parameters to be similar but higher in jejunal compared with ileal tissue. No such correlation was seen when comparing measurements of SGL...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00246-9
更新日期:1997-04-07 00:00:00
abstract::The pET(scF11) plasmid was constructed comprising the gene of a single-chain antibody against human ferritin. This plasmid encodes the leader peptide pelB followed by the heavy chain variable V(H) domain, (Gly4Ser)3 linker peptide, and light chain variable V(L) domain. The correctly processed scF11 antibody was expres...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01601-9
更新日期:1998-12-28 00:00:00
abstract::The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80286-r
更新日期:1990-07-02 00:00:00
abstract::The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80190-4
更新日期:1987-09-28 00:00:00
abstract::In a previous investigation of the rDNA region in Tetrahymena pyriformis mitochondrial DNA, we identified a putative tRNA(Met) gene [Heinonen et al. (1987) J. Biol. Chem. 262, 2879-2887]. On the basis of Northern hybridization analyses, we suggested that this gene is expressed, even though the resulting tRNA would be ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00179-d
更新日期:1995-03-27 00:00:00
abstract::The addition of hepatocyte growth factor (HGF) to rat hepatocytes in primary culture resulted in the formation of inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) and 1,2-diacylglycerol (DG) by a phosphoinositide-specific phospholipase C (PI-PLC). DG showed a biphasic increase; the first phase, corresponding with the peak ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80554-t
更新日期:1992-02-10 00:00:00
abstract::We propose a dynamic model for the competition between exon-exon ligation and 3'-end hydrolysis valid for sequentially folded pre-mRNA introns of group I. This model accounts for the delay in the formation of conserved helix P10 until the 5' exon has been cleaved, a requirement to prevent hydrolysis at the 3' end of t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80360-s
更新日期:1992-02-03 00:00:00
abstract::Glucose-regulated protein 58 (GRP58)-like immunoreactivity in rat liver obtained in the evening or after fasting underwent an electrophoretic band-shift, which disappeared after phosphatase-treatment. Since mass spectrometric analysis raised a possibility that Ser150 of GRP58 is phosphorylated, an antibody against the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.11.074
更新日期:2006-01-09 00:00:00
abstract::Chlorella virus-encoded membrane protein Kcv represents a new class of potassium channel. This 94-amino acids miniature K(+) channel consists of two trans-membrane alpha-helix domains intermediated by a pore domain that contains a highly conserved K(+) selectivity filter. Therefore, as an archetypal K(+) channel, the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.02.005
更新日期:2007-03-06 00:00:00
abstract::Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81119-s
更新日期:1991-10-07 00:00:00
abstract::Phosphorylation of histone variant H2AX at serine 139, named gammaH2AX, has been widely used as a sensitive marker for DNA double-strand breaks (DSBs). gammaH2AX is required for the accumulation of many DNA damage response (DDR) proteins at DSBs. Thus it is believed to be the principal signaling protein involved in DD...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.05.021
更新日期:2010-09-10 00:00:00
abstract::We investigated whether activation of integrin receptors could modulate the expression of monocyte chemotactic protein-1 (MCP-1) in human hepatic stellate cells (HSC), mesenchymal cells responsible for extracellular matrix synthesis within the liver. When compared to non-adherent cells, HSC plated on collagen types I ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01004-1
更新日期:1997-09-08 00:00:00
abstract::We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.05.044
更新日期:2006-06-26 00:00:00
abstract::The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription fac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.017
更新日期:2008-03-05 00:00:00
abstract::The function of the domains of Trichoderma reesei endoglucanase I (EGI) has been studied. Truncated EGI proteins were expressed from the 3'-end deleted cDNAs in the yeast Saccharomyces cerevisiae under the control of the ADC1 expression cassette. EGI protein was detected by monoclonal antibody EI-2 and EGI activity as...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81100-m
更新日期:1991-10-07 00:00:00
abstract::In blood-borne metastasis, intravasated metastatic tumor cells are thought to localize at the target site via a series of processes involving platelet aggregation, adhesion to endothelium, and invasion through the basal membrane. In the present study, we examined how platelet aggregation contributes to the trafficking...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00441-4
更新日期:1998-05-08 00:00:00
abstract::Human cystatin C (HCC) is a low molecular weight member of the cystatin family (type2). HCC consists of 120 amino acids. Normally it is an inhibitor of cysteine proteases, but in pathological conditions it forms amyloid fibrils in brain arteries of young adults. An 'aggregation-prone' pentapeptide ((47)LQVVR(51)) was ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.11.041
更新日期:2015-01-02 00:00:00
abstract::The 3-nitrosobenzamide (NOBA) drug abolishes SIV replication sharply at 20 microM concentration when CEM x 174 cells are preincubated for 1 h with the drug prior to viral infection. Treatment of CEM x 174 cells with 20 microM NOBA resulted in the inhibition of the synthesis of the DNA sequence coding for the gag gene,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81778-x
更新日期:1993-07-12 00:00:00
abstract::The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dyn...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03207-0
更新日期:2002-01-02 00:00:00
abstract::We have recently developed an in vitro differentiation model of immortalized non-transformed human hepatocytes using butyrate, and observed the induction of inducible NO synthase (iNOS). In this study, we analyzed the effect of NO on the urea-synthetic capacity of these cells. The inhibition of iNOS during butyrate tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01599-4
更新日期:2000-06-02 00:00:00
abstract::Sucrose synthase (SuSy) plays an important role in sucrose degradation and occurs both as a soluble and as a membrane-associated enzyme in higher plants. We show that membrane association can vary in vivo in response to gravistimulation, apparently involving SuSy dephosphorylation, and is a reversible process in vitro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01506-8
更新日期:1997-12-29 00:00:00
abstract::Acetylcholine (1-10 microM) depolarized the membrane and stimulated glucose-induced bursts of electrical activity in mouse pancreatic B-cells. The acetylcholine effects were mimicked by muscarine while nicotine had no effect on membrane potential. Pirenzepine, an antagonist of the classical M1-type muscarinic receptor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80669-6
更新日期:1989-06-05 00:00:00
abstract::Plasminogen activation is regulated by the interaction between urokinase-type plasminogen activator (uPA) and its specific glycolipid-anchored cell surface receptor (uPAR). uPAR is composed of three homologous domains and is the only multi-domain member of the Ly-6 family of glycolipid-anchored membrane proteins. Rece...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(94)00674-1
更新日期:1994-08-01 00:00:00
abstract::The contact-dependent intrinsic pathway of fibrinolysis involving factor XII, prekallikrein (PK) and pro-urokinase (pro-UK) remains poorly understood. Casein autography of washed, intact platelets revealed both PK and pro-UK. Accordingly, platelets may mediate physiological thrombolysis by this pathway since factor XI...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80537-5
更新日期:1993-03-08 00:00:00