Abstract:
:Human cystatin C (HCC) is a low molecular weight member of the cystatin family (type2). HCC consists of 120 amino acids. Normally it is an inhibitor of cysteine proteases, but in pathological conditions it forms amyloid fibrils in brain arteries of young adults. An 'aggregation-prone' pentapeptide ((47)LQVVR(51)) was located within the HCC sequence using AmylPred, an 'aggregation-prone' peptide prediction algorithm developed in our lab. This peptide was synthesized and self-assembled into amyloid-like fibrils in vitro, as electron microscopy, X-ray fiber diffraction, Attenuated Total Reflectance Fourier-Transform Spectroscopy and Congo red staining studies reveal. Thus, the (47)LQVVR(51) peptide seems to have an important role in HCC fibrillization.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tsiolaki PL,Hamodrakas SJ,Iconomidou VAdoi
10.1016/j.febslet.2014.11.041subject
Has Abstractpub_date
2015-01-02 00:00:00pages
159-64issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(14)00857-6journal_volume
589pub_type
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