Abstract:
:Glucose-fructose oxidoreductase (GFOR), a periplasmic protein of Zymomonas mobilis, is synthesized as a precursor polypeptide with a twin-R signal peptide for Sec-independent protein export in bacteria. In higher plant chloroplasts, twin-R signal peptides are specific targeting signals for the Sec-independent delta pH pathway of the thylakoid membrane system. In agreement with the assumed common phylogenetic origin of the two protein transport mechanisms, GFOR can be efficiently translocated by the delta pH-dependent pathway when analyzed with isolated thylakoid membranes. Transport is sensitive to the ionophore nigericin and competes with specific substrates for the delta pH-dependent transport route. In contrast, neither sodium azide nor enzymatic destruction of the nucleoside triphosphates in the assays affects thylakoid transport of GFOR indicating that the Sec apparatus is not involved in this process. Mutagenesis of the twin-R motif in the GFOR signal peptide prevents membrane translocation of the protein emphasizing the importance of these residues for the transport process.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Halbig D,Hou B,Freudl R,Sprenger GA,Klösgen RBdoi
10.1016/s0014-5793(99)00269-0keywords:
subject
Has Abstractpub_date
1999-03-19 00:00:00pages
95-8issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00269-0journal_volume
447pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilis...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81619-b
更新日期:1993-12-20 00:00:00
abstract::In plants exposed to high irradiances of visible light, the D1 protein in the reaction center of photosystem II is oxidatively damaged and rapidly degraded. Earlier work in our laboratory showed that the serine protease Deg2 performs the primary cleavage of photodamaged D1 protein in vitro. Here, we demonstrate that t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.11.058
更新日期:2006-12-22 00:00:00
abstract::We present here a combined GC-MS and LC-MS metabolic profiling approach to unraveling the pathological outcomes of aristolochic acid (AA)-induced nephrotoxicity. Urine samples were analyzed by GC-MS and LC-MS in combination with pattern recognition techniques, e.g. principal component analysis (PCA), orthogonal projec...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.01.036
更新日期:2007-02-20 00:00:00
abstract::The requirement for a proline residue at the C-terminus of the Ca(2+)-binding photoprotein, aequorin, was investigated by measuring luminescence activities of a series of C-terminal deletion mutants, substitution mutants and an addition mutant. CD spectral measurements of apoaequorin with the C-terminal proline delete...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81385-l
更新日期:1991-12-16 00:00:00
abstract::As yet, the physiological significance of hydroxylation of anandamide and linoleoyl amides is unknown. Therefore, we investigated whether hydroxylation of ODNHEtOH and ODNH2 influences their binding abilities to the CB-1 receptor and whether it alters their reactivity towards a fatty acid amide hydrolase (FAAH) from r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01148-4
更新日期:1997-10-06 00:00:00
abstract::A wide variety of phospholipase A2 inhibitors blocks intra-Golgi protein transport reconstituted in a cell-free system. Phospholipase A2 activity detectable under the protein transport assay conditions is actually inhibited by the inhibitors. There is a good correlation between the inhibition of protein transport and ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81393-e
更新日期:1993-06-14 00:00:00
abstract::Rhodamine 6G (3 microM) effectively inhibited delta pH-driven ATP synthesis in Methanobacterium thermoautotrophicum while delta pNa-driven ATP synthesis was not affected by it. Rhodamine 6G inhibited Mg(2+)-stimulated ATPase activity of membrane vesicles prepared from these cells but the ATPase catalytic sector detach...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00535-4
更新日期:1994-06-27 00:00:00
abstract::The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03346-x
更新日期:2002-10-23 00:00:00
abstract::The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.12.009
更新日期:2012-01-20 00:00:00
abstract::A major difference between porcine and bovine pancreatic phospholipase A2 (PA2) is the relatively low affinity of the bovine enzyme for lipid-water interfaces. We have investigated the binding of porcine, bovine, and equine PA2 to n-hexadecylphosphocholine (C16-PC) micelles using optically detected magnetic resonance ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81279-6
更新日期:1987-01-19 00:00:00
abstract::HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.09.026
更新日期:2011-10-20 00:00:00
abstract::The release of alanine and glutamine from perfused muscle of trained and control animals was investigated. Release rates did not differ between trained and control muscle at rest. During contractions in trained muscle, alanine release was higher than at rest, while glutamine release was transiently increased. Phenylal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80155-x
更新日期:1994-03-07 00:00:00
abstract::The CLN3 gene encodes an integral membrane protein of unknown function. Mutations in CLN3 can cause juvenile neuronal ceroid lipofuscinosis, or Batten disease, an inherited neurodegenerative lysosomal storage disease affecting children. Here, we report a topological study of the CLN3 protein using bioinformatic approa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.02.049
更新日期:2008-04-02 00:00:00
abstract::Human hepatocellular carcinoma cells (Hep G2) were shown to secrete apo A-I as a proprotein . No apo A-I synthesis could be detected with endothelial cells from human umbilical cord veins. Conversion of proapo A-I into apo A-I is a slow (of the order of hours) process, mediated by a Ca2+/Mg2+-dependent enzyme which is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81379-4
更新日期:1984-05-07 00:00:00
abstract::Fibroblasts were exposed to various concentrations of hydrogen peroxide and the removal of oxidized proteins was followed by determining protein-bound carbonyls. Fibroblasts are able to increase the turnover of metabolically radiolabeled proteins after treatment with hydrogen peroxide. It was demonstrated for the firs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01495-1
更新日期:1998-12-04 00:00:00
abstract::The changes in phosphoproteins purified with the affinity peptide p9CKShs1 were analyzed from extracts of regenerating rat livers in order to define some G1 and G1/S regulations characteristic of mature hepatocytes stimulated to proliferate. We observed a 47 kDa phosphoprotein that occurred first at the end of G1 befo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00629-8
更新日期:1999-06-11 00:00:00
abstract::We present here a new set of aminoglycoside-arginine conjugates (AACs) that are either site-specific or per-arginine conjugates of paromomycin, neamine, and neomycin B as well as their structure-activity relationships. Their binding constants (KD) for TAR and RRE RNAs, measured by fluorescence anisotropy, revealed dep...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.038
更新日期:2004-11-19 00:00:00
abstract::The accumulation of amyloid-beta (Aβ) and tau aggregates is a pathological hallmark of Alzheimer's disease. Both polypeptides form fibrillar deposits, but several lines of evidence indicate that Aβ and tau form toxic oligomeric aggregation intermediates. Depleting such structures could thus be a powerful therapeutic s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.11.026
更新日期:2015-01-02 00:00:00
abstract::Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00477-q
更新日期:1995-06-05 00:00:00
abstract::ADP-forming acetyl-CoA synthetase (ACD) catalyzes the interconversion of acetyl-CoA and acetate. The related succinyl-CoA synthetase follows a three-step mechanism involving a single phosphoenzyme, but a novel four-step mechanism with two phosphoenzyme intermediates was proposed for Pyrococcus ACD. Characterization of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.12573
更新日期:2017-02-01 00:00:00
abstract::A novel gene named fad104 (factor for adipocyte differentiation-104), whose expression level quickly increased in the early stage of adipogenesis, was isolated and characterized. The deduced amino acid sequence of fad104 revealed the possible presence of a fibronectin type III domain and transmembrane domain. The expr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.062
更新日期:2004-11-05 00:00:00
abstract::Plasminogen activation is regulated by the interaction between urokinase-type plasminogen activator (uPA) and its specific glycolipid-anchored cell surface receptor (uPAR). uPAR is composed of three homologous domains and is the only multi-domain member of the Ly-6 family of glycolipid-anchored membrane proteins. Rece...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(94)00674-1
更新日期:1994-08-01 00:00:00
abstract::The 36 kDa fragment of actin molecule obtained with the protease from E. coli A2 strain [(1988) FEBS Lett. 228, 172] was shown to begin with Val-43 and retain the COOH-terminal amino acid residues of the parent molecule. The E. coli protease split actin preserves the NH2-terminal part of the polypeptide chain as well ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80247-z
更新日期:1991-02-11 00:00:00
abstract::Treatment of HepG2 with all-trans retinoic acid (RA) induces expression of fatty acid synthase (FAS) mRNA and protein. Transfections show that the FAS promoter positively responds to retinoid X receptor (RXR) but not to RA receptor (RAR) agonists. Since RXR alone is capable of mediating the RA response of FAS, the exi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.022
更新日期:2007-06-12 00:00:00
abstract::Protein misfolding and aggregation are linked to several degenerative diseases and are responsible for the formation of bacterial inclusion bodies. Roles of molecular chaperones in promoting protein deposition have been speculated but not proven in vivo. We have investigated the involvement of individual chaperones in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00126-1
更新日期:2003-02-27 00:00:00
abstract::In guinea pig caudate slices preloaded with [3H]choline, superfusion with medium containing Ca2+ and the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate) produced an increase in the Ca2+-dependent, depolarization-evoked release of acetylcholine (ACh), with no significant effect on the evoked Ca2+ influx. The ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80146-6
更新日期:1986-01-20 00:00:00
abstract::Misfolding of the prion protein yields amyloidogenic isoforms, and it shows exacerbating neuronal damage in neurodegenerative disorders including prion diseases. Pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal peptide (VIP) potently stimulate neuritogenesis and survival of neuronal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02886-7
更新日期:2002-07-03 00:00:00
abstract::Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01906-2
更新日期:2000-09-01 00:00:00
abstract::Myosin light chain kinase (MLCK) is the key regulator of cell motility and smooth muscle contraction in higher vertebrates. We searched for the features of the high molecular weight MLCK (MLCK-210) associated with its unique N-terminal sequence not found in a more ubiquitous lower molecular weight MLCK (MLCK-108). MLC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01591-4
更新日期:1999-12-10 00:00:00
abstract::Regulation of supra-macromolecular composition and catalytic activity of a heterodimeric enzyme, gamma-glutamyltransferase, in the system of Aerosol OT (sodium bis(2-ethylhexyl) sulfosuccinate) reversed micelles in octane were studied. Variation of the surfactant hydration degree (parameter, determining dimensions of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81388-o
更新日期:1991-12-16 00:00:00