Comparing the temperature dependence of FMN to heme electron transfer in full length and truncated inducible nitric oxide synthase proteins.


:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the temperature. Our previous viscosity study indicated that the IET process is conformationally gated, and Eyring equation was thus used to analyze the temperature dependence data. The obtained magnitude of activation entropy for the IET in the oxyFMN construct is only one-fifth of that for the holoenzyme. This indicates that the FMN domain in the holoenzyme needs to sample more conformations before the IET takes place, and that the FMN domain in the oxyFMN construct is better poised for efficient IET.




FEBS letters


Li W,Chen L,Fan W,Feng C




Has Abstract


2012-01-20 00:00:00














  • 2D crystal forms of annexin IV on lipid monolayers.

    abstract::Two-dimensional crystalline arrays of annexin IV were generated by interaction of the purified protein with a phospholipid monolayer. Image analysis of electron micrographs of the protein crystals, which diffracted to 3.5 nm respectively, revealed p6 and p3 symmetry. Annexin IV gave two crystal forms with unit cells o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Newman RH,Leonard K,Crumpton MJ

    更新日期:1991-02-11 00:00:00

  • Bacterial lectins, cell-cell recognition and infectious disease.

    abstract::Numerous bacterial strains produce surface lectins, commonly in the form of fimbriae that are filamentous assemblies of protein subunits. Among the best characterized of these are the type 1 (mannose specific) fimbrial lectins of Escherichia coli that consist almost exclusively of one class of subunit with a molecular...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Sharon N

    更新日期:1987-06-15 00:00:00

  • A common evolutionary origin of two elementary enzyme folds.

    abstract::The (beta alpha)(8)-barrel is the most frequent and most versatile fold among enzymes [Höcker et al., Curr. Opin. Biotechnol. 12 (2001) 376-381; Wierenga, FEBS Lett. 492 (2001) 193-198]. Structural and functional evidence suggests that (beta alpha)(8)-barrels evolved from an ancestral half-barrel, which consisted of f...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Höcker B,Schmidt S,Sterner R

    更新日期:2002-01-16 00:00:00

  • Ribonomic approaches to study the RNA-binding proteome.

    abstract::Gene expression is controlled through a complex interplay among mRNAs, non-coding RNAs and RNA-binding proteins (RBPs), which all assemble along with other RNA-associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known o...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Faoro C,Ataide SF

    更新日期:2014-10-16 00:00:00

  • Nitric oxide reacts with the ferryl-oxo catalytic intermediate of the CuB-lacking cytochrome bd terminal oxidase.

    abstract::Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Borisov VB,Forte E,Sarti P,Brunori M,Konstantinov AA,Giuffrè A

    更新日期:2006-09-04 00:00:00

  • The complete sequence of human preprocalcitonin.

    abstract::DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin-producing tumour, was inserted in the Pst site of pBR 322 by G-C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin-resistant clones wer...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Le Moullec JM,Jullienne A,Chenais J,Lasmoles F,Guliana JM,Milhaud G,Moukhtar MS

    更新日期:1984-02-13 00:00:00

  • ING4 induces G2/M cell cycle arrest and enhances the chemosensitivity to DNA-damage agents in HepG2 cells.

    abstract::The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to interact with tumor suppressor p53, p300 (a major component of histone acetyl transferase complexes), and p65(RelA) subunit of NF-kappaB. In this st...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zhang X,Xu LS,Wang ZQ,Wang KS,Li N,Cheng ZH,Huang SZ,Wei DZ,Han ZG

    更新日期:2004-07-16 00:00:00

  • Protein-based phylogenetic analysis by using hydropathy profile of amino acids.

    abstract::So far, various approaches for phylogenetic analysis have been developed. Almost all of them put stress on analyzing nucleic acid sequences or protein primary structures. In this paper, we take the physicochemical properties of amino acids into account and introduce the hydropathy profile of amino acids into phylogene...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Liu N,Wang T

    更新日期:2006-10-02 00:00:00

  • STD and TRNOESY NMR studies for the epitope mapping of the phosphorylation motif of the oncogenic protein beta-catenin recognized by a selective monoclonal antibody.

    abstract::The interaction of the P-beta-Cat(19-44) peptide, a 26 amino acid peptide (K(19)AAVSHWQQQSYLDpSGIHpSGATTTAP(44)) that mimics the phosphorylated beta-Catenin antigen, has been studied with its monoclonal antibody BC-22, by transferred nuclear Overhauser effect NMR spectroscopy (TRNOESY) and saturation transfer differen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Megy S,Bertho G,Gharbi-Benarous J,Baleux F,Benarous R,Girault JP

    更新日期:2006-10-02 00:00:00

  • Prooxidant iron and copper, with ferroxidase and xanthine oxidase activities in human atherosclerotic material.

    abstract::Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Swain J,Gutteridge JM

    更新日期:1995-07-24 00:00:00

  • Specific inhibition of human beta-D-glucuronidase and alpha-L-iduronidase by a trihydroxy pipecolic acid of plant origin.

    abstract::The glucuronic acid analogue of 1-deoxynojirimycin, 2(S)-carboxy-3(R), 4(R), 5(S)-trihydroxypiperidine, recently isolated from seeds of Baphia racemosa, is a novel specific inhibitor of human liver beta-D-glucuronidase and alpha-L-iduronidase. No other glycosidases are inhibited. The inhibition of beta-D-glucuronidase...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Cenci di Bello I,Dorling P,Fellows L,Winchester B

    更新日期:1984-10-15 00:00:00

  • Phosphorylation of plant actin-depolymerising factor by calmodulin-like domain protein kinase.

    abstract::The actin-depolymerising factor (ADF)/cofilin group of proteins are stimulus-responsive actin-severing proteins, members of which are regulated by reversible phosphorylation. The phosphorylation site on the maize ADF, ZmADF3, is Ser-6 but the kinase responsible is unknown [Smertenko et al., Plant J. 14 (1998) 187-193]...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Allwood EG,Smertenko AP,Hussey PJ

    更新日期:2001-06-15 00:00:00

  • Effects of analogues of adenosine and methyl xanthines on insulin sensitivity in soleus muscle of the rat.

    abstract::The concentration of insulin that produces half-maximal stimulation of glycolysis by stripped soleus muscle preparations is markedly increased by the adenosine analogues, 2-chloroadenosine and N6-phenylisopropyladenosine, but is markedly decreased by the methyl xanthine analogue, 8-phenyltheophylline. 2-Chloroadenosin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Budohoski L,Challiss RA,McManus B,Newsholme EA

    更新日期:1984-02-13 00:00:00

  • Studies of a putative ice-binding motif in winter flounder skin-type anti-freeze polypeptide.

    abstract::Winter flounder contains two distinct anti-freeze protein isoforms, which are the liver-type extracellular anti-freeze proteins and the skin-type intracellular anti-freeze protein. The skin-type anti-freeze proteins exhibit lower anti-freeze activities than the liver-type isoforms and this might be due to their lackin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lin Q,Ewart KV,Yang DS,Hew CL

    更新日期:1999-06-25 00:00:00

  • Feedback regulation of Ras2 guanine nucleotide exchange factor (Ras2-GEF) activity of Cdc25p by Cdc25p phosphorylation in the yeast Saccharomyces cerevisiae.

    abstract::Ras-GEF Cdc25p has been found to be hyperphosphorylated upon glucose addition. This work provides evidence indicating that PKA activity positively regulates the degree of Cdc25p phosphorylation, and that the intracellular association of Cdc25p and Ras2p is independent of PKA activity. In vitro experiments revealed tha...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jian D,Aili Z,Xiaojia B,Huansheng Z,Yun H

    更新日期:2010-12-01 00:00:00

  • Structural modifications associated with the change in Ca2+ sensitivity on activation of m-calpain.

    abstract::Autolysis of the Ca(2+)-dependent cysteine protease m-calpain involves cleavage of the large (80 kDa) and small (30 kDa) subunits of the enzyme, and an increase in Ca2+ sensitivity. The appearance of increased Ca2+ sensitivity was found to correlate with the cleavage of the large subunit after residue 9. ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brown N,Crawford C

    更新日期:1993-05-03 00:00:00

  • The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma.

    abstract::BSP-30K is a major acidic glycoprotein of bovine seminal plasma. It displays heparin-, gelatin-, and phospholipid-binding activities. BSP-30K binds to spermatozoa upon ejaculation and is thought to play a role in sperm capacitation. We have determined its amino acid sequence, disulfide bonds, and 0-glycosylation sites...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Calvete JJ,Mann K,Sanz L,Raida M,Töpfer-Petersen E

    更新日期:1996-12-09 00:00:00

  • Synthesis and biological evaluation of substituted phosphate triester alkyl lyso phospholipids (ALPs) as novel potential anti-neoplastic agents.

    abstract::Phosphate triester derivatives of the anti-neoplastic alkyl lyso phospholipid (ALP) have been prepared as novel potential therapeutic agents. In particular, symmetrical phosphate triesters have been prepared, using phosphorochloridate chemistry. The compounds have been fully characterised by a range of techniques, and...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McGuigan C,Wang Y,Riley PA

    更新日期:1995-09-25 00:00:00

  • Steroid hormones and temperature induce changes of binding parameters of their receptors in intact cells.

    abstract::When MCF-7 cells were treated with 17 beta-estradiol, dexamethasone, or promegestone at 37 degrees C, the KD of receptors for their cognate ligands was found to decrease as compared to that measured at 2 degrees C. Cell incubation with hormone at 37 degrees C did not affect the Bmax of glucocorticoid and progesterone ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rossini GP,Malaguti C

    更新日期:1995-12-04 00:00:00

  • Structurally conserved and functionally divergent yeast Ssu72 phosphatases.

    abstract::The eukaryotic Ssu72 factor is involved in several RNA biogenesis processes. It has phosphatase activity on the carboxy-terminal domain (CTD) of the major subunit of RNA polymerase II. The Kluyveromyces lactis Ssu72 (KlSsu72) shows in vitro phosphatase activity for the pNPP substrate, and this activity is inhibited by...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rodríguez-Torres AM,Lamas-Maceiras M,García-Díaz R,Freire-Picos MA

    更新日期:2013-08-19 00:00:00

  • Modulation of O2 reduction in Saccharomyces cerevisiae mitochondria.

    abstract::Respiratory supercomplex factor (Rcf) 1 is a membrane-bound protein that modulates the activity of cytochrome c oxidase (CytcO) in Saccharomyces cerevisiae mitochondria. To investigate this regulatory mechanism, we studied the interactions of CytcO with potassium cyanide (KCN) upon removal of Rcf1. While the addition ...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Rydström Lundin C,Brzezinski P

    更新日期:2017-12-01 00:00:00

  • A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium.

    abstract::Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Fischer M,Haase I,Simmeth E,Gerisch G,Müller-Taubenberger A

    更新日期:2004-11-05 00:00:00

  • The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: molecular and physiological characterization of PEPC kinase mutants.

    abstract::Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meimoun P,Gousset-Dupont A,Lebouteiller B,Ambard-Bretteville F,Besin E,Lelarge C,Mauve C,Hodges M,Vidal J

    更新日期:2009-05-19 00:00:00

  • Visualisation by electron microscopy of the unique part of the cytoplasmic domain of a desmoglein, a cadherin-like protein of the desmosome type of cell junction.

    abstract::Part of the cytoplasmic domain of a human desmoglein, Dsg1, a cadherin-like protein found in desmosomes of epithelial cells, has been visualised by electron microscopy. The cloned fragment contains five repeats of a 29 +/- 4 residue sequence unique to desmogleins, followed by a glycine-rich region. In rotary shadowed ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rutman AJ,Buxton RS,Burdett ID

    更新日期:1994-10-17 00:00:00

  • The regulation of gene expression by insulin is differentially impaired in the liver of the genetically obese-hyperglycemic Wistar fatty rat.

    abstract::The regulation by insulin and carbohydrates of the gene expression of three key enzymes involved in glucose metabolism was studied in the liver of the Wistar fatty rat, a model of obese non-insulin-dependent diabetes mellitus. A high glucose or fructose diet, or insulin administration caused a similar magnitude of inc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Noguchi T,Matsuda T,Tomari Y,Yamada K,Imai E,Wang Z,Ikeda H,Tanaka T

    更新日期:1993-08-09 00:00:00

  • Proteasome-dependent degradation of oxidized proteins in MRC-5 fibroblasts.

    abstract::Fibroblasts were exposed to various concentrations of hydrogen peroxide and the removal of oxidized proteins was followed by determining protein-bound carbonyls. Fibroblasts are able to increase the turnover of metabolically radiolabeled proteins after treatment with hydrogen peroxide. It was demonstrated for the firs...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sitte N,Merker K,Grune T

    更新日期:1998-12-04 00:00:00

  • Association of the polymorphisms in the 5'-untranslated region of PTEN gene with type 2 diabetes in a Japanese population.

    abstract::Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) is known to act as a lipid phosphatase hydrolyzing phosphatidylinositol (PI)(3,4,5)P(3) to PI(4,5)P(2). Since the PI3-kinase product, PI(3,4,5)P(3), is an important second messenger leading to the metabolic action of insulin, PTEN functions as a potent neg...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishihara H,Sasaoka T,Kagawa S,Murakami S,Fukui K,Kawagishi Y,Yamazaki K,Sato A,Iwata M,Urakaze M,Ishiki M,Wada T,Yaguchi S,Tsuneki H,Kimura I,Kobayashi M

    更新日期:2003-11-20 00:00:00

  • Identification of an uncoupling mutation affecting the b subunit of F1F0 ATP synthase in Escherichia coli.

    abstract::A specific b subunit arginine, b(Arg-36) in Escherichia coli, displays evolutionary conservation among bacterial F1F0 ATP synthases. Site-directed mutagenesis was used to generate a collection of mutations affecting b(Arg-36). The phenotype differed depending upon the substitution, and the b(Arg-36-Glu) and b(Arg-36-I...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Caviston TL,Ketchum CJ,Sorgen PL,Nakamoto RK,Cain BD

    更新日期:1998-06-12 00:00:00

  • Rapid purification of wildtype and mutant cytochrome c oxidase from Rhodobacter sphaeroides by Ni(2+)-NTA affinity chromatography.

    abstract::A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Mitchell DM,Gennis RB

    更新日期:1995-07-10 00:00:00

  • A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses.

    abstract::Statistically significant similarity was revealed between amino acid sequences of NTP-binding pattern-containing domains which are among the most conserved protein segments in dissimilar groups of ss and dsDNA viruses (papova-, parvo-, geminiviruses and P4 bacteriophage), and RNA viruses (picorna-, como- and nepovirus...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gorbalenya AE,Koonin EV,Wolf YI

    更新日期:1990-03-12 00:00:00