Abstract:
:The role of the RNA-binding protein human antigen R (HuR) in hepatocarcinogenesis is still elusive. By employing short hairpin (sh)RNA-dependent knockdown approach, we demonstrate that lymphotoxin α (LTα) is a target of posttranscriptional gene regulation by HuR in hepatocellular carcinoma (HepG2) cells. Consequently, the increased mRNA decay upon HuR depletion significantly affects lymphotoxin expression at both, the mRNA and protein level. Biotin-pulldown assay showed that HuR specifically interacts with the 3'-untranslated region (3'-UTR) of the LTα mRNA. Furthermore, electrophoretic mobility shift assay (EMSA) implicates that the RNA-binding critically depends on the RNA recognition motif 2 (RRM2) and the hinge region of HuR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Steinmeyer N,Doller A,Biyanee A,Brauss T,Schmid T,Pfeilschifter J,Eberhardt Wdoi
10.1016/j.febslet.2015.05.015subject
Has Abstractpub_date
2015-07-08 00:00:00pages
1943-50issue
15eissn
0014-5793issn
1873-3468pii
S0014-5793(15)00391-9journal_volume
589pub_type
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