Abstract:
:The protozoan parasite and human pathogen Entamoeba histolytica is protected against killing by its own lytic effector proteins. Amoebae withstand doses of amoebapores, their pore-forming polypeptides, that readily kill human Jurkat T cells. Moreover, the polypeptides do not bind to the amoebic surface membrane as evidenced by using fluorescently labelled amoebapores and confocal laser microscopy. Experiments employing liposomes as a minimalistic membrane system and the major isoform amoebapore A revealed that the lipid composition of amoebic membranes prevents binding of the cytolytic molecule and that both the phospholipid ingredients and the high content of cholesterol contributes to the protection of the toxin-producing cell.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Andrä J,Berninghausen O,Leippe Mdoi
10.1016/S0014-5793(04)00324-2keywords:
subject
Has Abstractpub_date
2004-04-23 00:00:00pages
109-15issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579304003242journal_volume
564pub_type
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