Abstract:
:Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development correlated with the onset of transcription. Nevertheless, highly methylated ribosomal genes were transcribed at normal levels when injected into oocyte nuclei, and thus transcription of these genes was generally assumed to be insensitive to CpG-methylation. Here I show that Xenopus ribosomal gene transcription can be repressed by cellular factors binding to meCpG, similarly as it has been described for transcription by RNA polymerase II. In the absence of these repressors, however, CpG-methylation has a direct positive effect on RNA polymerase I-promoter activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Labhart Pdoi
10.1016/0014-5793(94)01291-1subject
Has Abstractpub_date
1994-12-19 00:00:00pages
302-6issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(94)01291-1journal_volume
356pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Coding region and intronic mutations in the gene for microtubule-associated protein tau cause frontotemporal dementia and Parkinsonism linked to chromosome 17 (FTDP-17). Most coding region mutations effect a reduced ability of tau protein to interact with microtubules and lead to the formation of a filamentous patholo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02169-4
更新日期:2000-11-10 00:00:00
abstract::It has been suggested that alpha-dystroglycan links the dystrophin-associated protein complex and extracellular matrix and that the absence of dystrophin and alpha-dystroglycan in Duchenne muscular dystrophy (DMD) may lead to the breakdown of this linkage. In the present study, myotubes from DMD patients and murine X-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00460-2
更新日期:1997-05-26 00:00:00
abstract::In bovine adrenocortical cells, the fatty acyl chains of the phospholipids have been identified in the membranes of the different cell compartments: plasma membranes, Golgi complex and coated vesicle membranes. An increase in the total number of unsaturation in the fatty acid is demonstrated in the coated vesicle memb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81201-2
更新日期:1987-07-13 00:00:00
abstract::Large conductance voltage-dependent and Ca(2+)-modulated K(+) channels play a crucial role in myometrium contractility. Western blots and immunocytochemistry of rat uterine sections or isolated cells show that MaxiK channel protein signals drastically decrease towards the end of pregnancy. Consistent with a transcript...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01394-0
更新日期:1999-11-05 00:00:00
abstract::We previously identified a benzyloxycarbonyl(Z)-Leu-Leu-Leu-4-methylcoumaryl-7-amide (ZLLL-MCA) degrading activity in proteasome as a candidate for the regulator of neurite outgrowth. As its counterpart, we purified a protein from bovine brain that specifically inhibits the ZLLL-MCA degrading activity in proteasome. T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00388-2
更新日期:1994-05-16 00:00:00
abstract::Inducible heat shock protein 70 (Hsp70) is one of the most important HSPs for maintenance of cell integrity during normal cellular growth as well as pathophysiological conditions. Tumor necrosis factor receptor-associated factor 6 (TRAF6) is a crucial signaling transducer that regulates a diverse array of physiologica...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.066
更新日期:2006-05-29 00:00:00
abstract::We identified a yeast mutant with temperature-sensitive growth defects that were rescued by VCP expression. The mutation occurred in GPI10, which encodes a mannosyl transferase for glycosylphosphatidylinositol anchor formation in the endoplasmic reticulum, and caused a Gly469Glu substitution in Gpi10. The mutant exhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.01.017
更新日期:2015-02-27 00:00:00
abstract::The bchH gene of Rhodobacter capsulatus has been cloned into an expression strain of Escherichia coli. Following induction of expression of the BchH protein, it was found that the E. coli strain also accumulated porphyrins with the fluorescence properties of protoporphyrin and zinc protoporphyrin. It was also found th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00371-2
更新日期:1997-05-05 00:00:00
abstract::The physical properties of amino acids were investigated in order to evaluate their possible relationship to the assignment of codons for amino acids in the genetic code. A comparison of the interconversion probability between amino acids and the distances between the amino acids for individual physical properties rev...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81237-2
更新日期:1989-04-10 00:00:00
abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80766-v
更新日期:1991-06-17 00:00:00
abstract::A link between alterations in cholesterol homeostasis and Alzheimer's disease (AD) is nowadays widely accepted. However, the molecular mechanism/s underlying such link remain unclear. Numerous experimental evidences support the view that changes in neuronal membrane cholesterol levels and/or subcellular distribution d...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.06.038
更新日期:2006-10-09 00:00:00
abstract::In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(87)80664-6
更新日期:1987-06-15 00:00:00
abstract::The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT-Raman spectroscopy in reaction centres purified from an antenna-deficient strain of Rhodobacter sphaeroides. We find that mutation at the M210 position does not significantly perturb th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80376-5
更新日期:1994-02-14 00:00:00
abstract::Ribosomal protection proteins (RPPs) confer bacterial resistance to tetracycline by releasing this antibiotic from ribosomes stalled in protein synthesis. RPPs share structural similarity to elongation factor G (EF-G), which promotes ribosomal translocation during normal protein synthesis. We constructed and functiona...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.03.023
更新日期:2008-04-16 00:00:00
abstract::Phosphorylation of the basal site with glycogen synthase kinase-4 enhanced the rate of phosphorylation of the regulatory site by cyclic AMP-dependent protein kinase 1.7-fold. In contrast, the phosphorylation state of the regulatory site did not affect the rate of phosphorylation of the basal site with glycogen synthas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80390-9
更新日期:1988-05-09 00:00:00
abstract::An abundant matrix protein was purified under native conditions from adult bovine tendon and identified as cartilage oligomeric matrix protein (COMP) by immunochemical crossreaction, amino acid sequence identity of tryptic peptides derived from both N- and C-terminal regions, and structure revealed by electron microsc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01134-6
更新日期:1994-11-07 00:00:00
abstract::To assure fidelity of translation, class Ia aminoacyl-tRNA synthetases (aaRSs) edit mis-aminoacylated tRNAs. Mis-attached amino acids and structural water molecules are not included simultaneously in the current crystal structures of the aaRSctRNA complexes, where the 3'-ends (adenine 76; A76) are bound to the editing...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.01.049
更新日期:2009-02-18 00:00:00
abstract::Replacement of tryptophan 392 located in the active site cavity of pyruvate decarboxylase (PDC; EC 4.1.1.1) from Zymomonas mobilis by methionine or glutamine yields enzymes with smaller catalytic constants of 8.5 s(-1) and 3.6 s(-1) at 4 degrees C, compared to that of the wild-type enzyme (17 s(-1)). The rate constant...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01594-4
更新日期:1998-12-28 00:00:00
abstract::Sufficient highly purified native pea cytosolic ascorbate peroxidase was obtained to characterize some of its kinetic and spectral properties. Its rate constant for compound I formation from reaction with H2O2 is 4.O x 10(7) M-1 s-1, somewhat faster than is typical for peroxidases. Compound I has the typical optical s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00562-5
更新日期:1996-07-01 00:00:00
abstract::Micromolar concentrations of arachidonic acid cause in Ca2+ loaded heart mitochondria matrix swelling and Ca2+ release. These effects appear to be unrelated to the classical membrane permeability transition (MPT), as they are CsA insensitive, membrane potential independent and can also be activated by Sr2+. Atractylos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.090
更新日期:2006-02-06 00:00:00
abstract::HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80045-9
更新日期:1984-01-23 00:00:00
abstract::Polysulfide is a bound sulfur species derived from endogenous H2S. When mouse neuroblastoma, Neuro2A cells were exposed to tert-butyl hydroperoxide after treatment with polysulfide, a significant decline in cell toxicity was observed. Rapid uptake of polysulfides induced translocation of Nrf2 into the nucleus, resulti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.09.013
更新日期:2013-11-01 00:00:00
abstract::Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O(2)(-)). Here, we analyzed the O(2)(-) sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted for O(2)(-)-dependent NO degradation by mi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.06.012
更新日期:2009-07-07 00:00:00
abstract::Using a monoclonal antibody as conformational probe it has been shown that the weakly active nicked-beta 2 dimer of tryptophan synthase generated by proteolytic cleavage at Glu-296, does not undergo on association with alpha subunit a conformational change known to occur in intact beta 2 subunit. This alpha induced co...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80591-h
更新日期:1993-04-12 00:00:00
abstract::Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROalpha, MCP-1, RANTES, MIP-1alpha and MIP-1beta expression was evaluat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00886-8
更新日期:1999-07-23 00:00:00
abstract::Pathway analysis methods are frequently applied to cancer gene expression data to identify dysregulated pathways. These methods often infer pathway activity based on the expression of genes belonging to a given pathway, even though the proteins ultimately determine the activity of a given pathway. Furthermore, the ass...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.14011
更新日期:2020-12-01 00:00:00
abstract::Capsaicin, a major ingredient of hot pepper, is considered to exhibit anti-inflammatory properties. Our previous study demonstrated that capsaicin inhibited the production of pro-inflammatory mediators through NF-kappaB inactivation in LPS-stimulated macrophages. In order to further clarify the mechanism underlying th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.06.084
更新日期:2004-08-13 00:00:00
abstract::MM-1 has been reported to repress the E-box-dependent transcription activity of c-Myc by recruiting histone deacetylase 1 complex via TIF1beta/KAP1. In this study, to identify target genes for c-Myc-MM-1-TIF1beta, we established rat-1 cells harboring the dominant-negative form of TIF1beta to abrogate the pathway from ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.07.034
更新日期:2004-08-13 00:00:00
abstract::Stroke is characterized by massive inflammation in areas surrounding the injury that magnifies damage to the brain. The liver X receptors (LXRs) are nuclear receptors that regulate cholesterol, lipid, and glucose metabolism. Synthetic LXR agonists have potent anti-inflammatory properties in a variety of settings, incl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.08.035
更新日期:2008-10-15 00:00:00
abstract::1,3-alpha-D-Glucan synthase of Streptococcus mutans catalyzes both the hydrolysis of sucrose to glucose and fructose, and the glucosyl transfer to glucosyl polymers to yield water-insoluble glucan. The enzyme catalyzes only sucrose hydrolysis, however, in the absence of 1,6-alpha-D-glucan as an acceptor. In the presen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80158-9
更新日期:1989-01-30 00:00:00