Abstract:
:Phosphorylation of the basal site with glycogen synthase kinase-4 enhanced the rate of phosphorylation of the regulatory site by cyclic AMP-dependent protein kinase 1.7-fold. In contrast, the phosphorylation state of the regulatory site did not affect the rate of phosphorylation of the basal site with glycogen synthase kinase-4. The rate of dephosphorylation of either the regulatory or the basal phosphorylation site by protein phosphatase-1, 2A or 2C was independent of the phosphorylation state of the other site. These results suggest that the basal phosphorylation site could play an indirect role in the control of the hormone-sensitive lipase activity in the adipocyte by functioning as a recognition site for the cyclic AMP-dependent protein kinase in the phosphorylation of the activity-controlling regulatory phosphorylation site in response to lipolytic hormones.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Olsson H,Belfrage Pdoi
10.1016/0014-5793(88)80390-9subject
Has Abstractpub_date
1988-05-09 00:00:00pages
78-82issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(88)80390-9journal_volume
232pub_type
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