Hsp70 inhibits lipopolysaccharide-induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination.

abstract：:alpha-Crystallin, the major protein of the ocular lens, is known to have extensive similarities to small heat shock proteins and to act as a molecular chaperone. The exposure of hydrophobic surfaces on alpha-crystallin was studied by fluorescence spectroscopy using the hydrophobic probe bis-ANS. Upon heating the prote...

journal_title：FEBS letters

authors： Das KP,Surewicz WK

更新日期：1995-08-07 00:00:00

abstract：:Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...

journal_title：FEBS letters

authors： Gether U,Marray T,Schwartz TW,Johansen TE

更新日期：1992-01-27 00:00:00

abstract：:The isolation of a novel biologically active peptide, designated galanin, is described. The peptide was discovered by the detection of its C-terminal amide structure in porcine intestinal extract using a chemical method. It was found that galanin consists of 29 amino acids and the complete amino acid sequence is: Gly-...

journal_title：FEBS letters

authors： Tatemoto K,Rökaeus A,Jörnvall H,McDonald TJ,Mutt V

更新日期：1983-11-28 00:00:00

abstract：:The topic synthetic biology appears still as an 'empty basket to be filled'. However, there is already plenty of claims and visions, as well as convincing research strategies about the theme of synthetic biology. First of all, synthetic biology seems to be about the engineering of biology - about bottom-up and top-dow...

journal_title：FEBS letters

authors： Malinova V,Nallani M,Meier WP,Sinner EK

更新日期：2012-07-16 00:00:00

abstract：:Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to st...

journal_title：FEBS letters

authors： Gangoiti P,Granado MH,Arana L,Ouro A,Gómez-Muñoz A

更新日期：2008-06-25 00:00:00

abstract：:To evaluate ozone damage to hairless mouse skin, two parameters of oxidative damage, vitamin E depletion and malondialdehyde (MDA) production, were measured in vitamin E-enriched and in control skin from mice exposed to ozone (10 ppm). A 5% vitamin E solution (tocotrienol-rich fraction, TRF) in polyethylene glycol (PE...

journal_title：FEBS letters

authors： Thiele JJ,Traber MG,Podda M,Tsang K,Cross CE,Packer L

更新日期：1997-01-20 00:00:00

abstract：:Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin...

journal_title：FEBS letters

authors： Kumari S,Dash D

更新日期：2011-07-21 00:00:00

abstract：:Acute myeloid leukemia (AML) associated translocations often cause gene fusions that encode oncofusion proteins. Although many of the breakpoints involved in chromosomal translocations have been cloned, in most cases the role of the chimeric proteins in tumorigenesis is not elucidated. Here we will discuss the fusion ...

journal_title：FEBS letters

authors： Martens JH,Stunnenberg HG

更新日期：2010-06-18 00:00:00

abstract：:Mitochondrial fatty acid synthesis is catalyzed by a dissociated fatty acid synthase similar to those of plant plastids and bacteria. The crystal structure of a mitochondrial beta-ketoacyl-[acyl carrier protein] synthase (mtKAS), namely that from Arabidopsis thaliana, has been determined for the first time. This enzym...

journal_title：FEBS letters

authors： Olsen JG,Rasmussen AV,von Wettstein-Knowles P,Henriksen A

更新日期：2004-11-05 00:00:00

abstract：:Physiological amyloid aggregation occurs within the nuclei of stress-treated cells. These structures, termed Amyloid bodies (A-bodies), assemble through the rapid accumulation of proteins into dense membrane-less organelles, which possess the same biophysical properties as plaques observed in many amyloid-based diseas...

journal_title：FEBS letters

authors： Marijan D,Tse R,Elliott K,Chandhok S,Luo M,Lacroix E,Audas TE

更新日期：2019-11-01 00:00:00

abstract：:The biological role of phosphoproteins depends upon their degree of phosphorylation in vivo. Methods currently available to measure the degree of phosphorylation of a protein involve indirect procedures to detect the 32P-phosphate incorporation. We report here a direct method to measure relative amounts of phospho- an...

journal_title：FEBS letters

authors： Di Luca M,de Graan PN,De Angelis L,Gispen WH,Cattabeni F

更新日期：1992-04-20 00:00:00

abstract：:The cytochrome bc(1) complex catalyzes electron transfer from ubiquinol to cytochrome c by a protonmotive Q cycle mechanism in which electron transfer is linked to proton translocation across the inner mitochondrial membrane. In the Q cycle mechanism proton translocation is the net result of topographically segregated...

journal_title：FEBS letters

authors： Hunte C,Palsdottir H,Trumpower BL

更新日期：2003-06-12 00:00:00

abstract：:DNA complementary to the rat hypothalamic mRNA coding for the corticotropin-releasing factor precursor (prepro-CRF) has been cloned by screening a cDNA library with a human genomic DNA probe. Nucleotide sequence analysis of the cloned cDNA has revealed that rat prepro-CRF consists of 187 amino acid residues including ...

journal_title：FEBS letters

authors： Jingami H,Mizuno N,Takahashi H,Shibahara S,Furutani Y,Imura H,Numa S

更新日期：1985-10-21 00:00:00

abstract：:The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by...

journal_title：FEBS letters

authors： Carrillo-Rayas T,Garcia-Soto J,Martinez-Cadena G

更新日期：1988-10-10 00:00:00

abstract：:Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions. We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions...

journal_title：FEBS letters

authors： Allen S,Davies J,Dawkes AC,Davies MC,Edwards JC,Parker MC,Roberts CJ,Sefton J,Tendler SJ,Williams PM

更新日期：1996-07-22 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:The photoreduction of the primary electron acceptor, QA, has been characterized by light-induced Fourier transform infrared difference spectroscopy for Rb. sphaeroides reaction centers and for Rsp. rubrum and Rp. viridis chromatophores. The samples were treated both with redox compounds, which rapidly reduce the photo...

journal_title：FEBS letters

authors： Breton J,Thibodeau DL,Berthomieu C,Mäntele W,Verméglio A,Nabedryk E

更新日期：1991-01-28 00:00:00

abstract：:The mucosal epithelium secretes a variety of antimicrobial peptides that act as part of the innate immune system to protect against invading microbes. Here, we describe the functional properties of human defensin (HD) 5, the major antimicrobial peptide produced by Paneth cells in the ileum, in relation to its structur...

journal_title：FEBS letters

authors： de Leeuw E,Burks SR,Li X,Kao JP,Lu W

更新日期：2007-02-06 00:00:00

abstract：:AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 tran...

journal_title：FEBS letters

authors： Zhang W,Xu Y,Chen Z,Xu Z,Xu H

更新日期：2011-10-03 00:00:00

abstract：:Treatment of either crude or purified preparations of the gamma-aminobutyrate (GABA)/benzodiazepine receptor complex with arginine-specific reagents resulted in a time- and concentration-dependent loss of [3H]muscimol binding activity. Following exposure to either 2,3-butanedione or phenylglyoxal (less than or equal t...

journal_title：FEBS letters

authors： Widdows KB,Kirkness EF,Turner AJ

更新日期：1987-09-28 00:00:00

abstract：:Detailed structural models of di-cluster seven-iron ferredoxins constitute a valuable resource for folding and stability studies relating the metal cofactors' role in protein stability. The here reported, hemihedric twinned crystal structure at 2.0 A resolution from Acidianus ambivalens ferredoxin, shows an integral 1...

journal_title：FEBS letters

authors： Frazão C,Aragão D,Coelho R,Leal SS,Gomes CM,Teixeira M,Carrondo MA

更新日期：2008-03-05 00:00:00

abstract：:Pexophagy is a selective autophagy process wherein damaged and/or superfluous peroxisomes undergo vacuolar degradation. In methylotropic yeasts, where pexophagy has been studied most extensively, this process occurs by either micro- or macropexophagy: processes analogous to micro- and macroautophagy. Recent studies ha...

journal_title：FEBS letters

authors： Manjithaya R,Nazarko TY,Farré JC,Subramani S

更新日期：2010-04-02 00:00:00

abstract：:MicroRNAs mainly inhibit coding genes and long non-coding RNA expression. Here, we report that hsa-miR-125b and oncogene SIRT7/oncogenic long non-coding RNA MALAT1 were inversely expressed in bladder cancer. Hsa-miR-125b mimic down-regulated, whereas hsa-miR-125b inhibitor up-regulated the expression of SIRT7 and MALA...

journal_title：FEBS letters

authors： Han Y,Liu Y,Zhang H,Wang T,Diao R,Jiang Z,Gui Y,Cai Z

更新日期：2013-11-29 00:00:00

abstract：:All eukaryotic cells enclose their genome within a dedicated, membrane-bound organelle termed the nucleus, which functions to partition gene transcription from sites of protein translation in the cytoplasm. Despite a great deal of research effort, basic questions about chromosome structure and gene expression mechanis...

journal_title：FEBS letters

authors： Trinkle-Mulcahy L,Lamond AI

更新日期：2008-06-18 00:00:00

abstract：:Association of protein inclusions or aggregates within brain tissues of patients with neurodegenerative disorders has been widely reported. These inclusions are commonly characterised both by the presence of ubiquitylated proteins and the sequestration of components of the ubiquitin-proteasome system (UPS). Such obser...

journal_title：FEBS letters

authors： Ardley HC,Hung CC,Robinson PA

更新日期：2005-01-31 00:00:00

abstract：:Resonance Raman spectroscopy was applied to monitor the effects of chloroquine (CQ) treatment on cultures of Plasmodium falciparum trophozoites. A number of bands assigned to A(1g) and B(1g) modes characteristic of the haemozoin aggregate are reduced in intensity in the CQ-treated cells, however, no bands from the CQ ...

journal_title：FEBS letters

authors： Webster GT,Tilley L,Deed S,McNaughton D,Wood BR

更新日期：2008-04-02 00:00:00

abstract：:Insulin causes a translocation of glucose transporters from intracellular microsomes to the plasma membrane in adipocytes. To determine whether insulin has a similar effect in rat hindlimb muscles, we used glucose-inhibitable cytochalasin B binding to estimate the number of glucose transporters in membrane fractions f...

journal_title：FEBS letters

authors： Klip A,Ramlal T,Young DA,Holloszy JO

更新日期：1987-11-16 00:00:00

abstract：:To investigate interactions between the polychlorinated biphenyl-binding protein uteroglobin and phospholipids, we used a liposome-pelletting assay. PCB-BP/uteroglobin bound to liposomes made from negatively charged phospholipids (PtdSer and PtdIns) in the presence of 5 mM calcium. No binding to liposomes made from ph...

journal_title：FEBS letters

authors： Nord M,Gustafsson JA,Lund J

更新日期：1995-11-06 00:00:00

abstract：:Using model spontaneously reverting cell lines, c-jun, junB, junD and c-fos oncogene expression was investigated. c-jun, but not junB, junD or c-fos, was overexpressed in highly tumorigenic clones. The reversion of cells to the non-tumorigenic phenotype resulted in a dramatic decrease in c-jun expression. CAT assays r...

journal_title：FEBS letters

authors： Lavrovsky Y,Yefremov Y,Lavrovsky V

更新日期：1994-12-19 00:00:00

abstract：:The non-ionic detergent Brij 58P is recommended as a stabilizing agent for protein storage; for example, the aggregation-prone chaperone DnaJ can be maintained in solution by low concentrations of Brij 58P. During protein folding studies with alpha-glucosidase, rhodanese and citrate synthase as model proteins, we disc...

journal_title：FEBS letters

authors： Krause M,Rudolph R,Schwarz E

更新日期：2002-12-04 00:00:00