Abstract:
:Several studies have indicated the existence of direct effects of melatonin on platelets. Here we show that, melatonin at high concentration is capable of significantly raising platelet intracellular calcium even in the absence of an agonist. The effect of melatonin on platelets was abolished by luzindole, a melatonin receptor blocker, and rotenone, while it was unaffected by cell-permeable antagonists of either inositol 1,4,5-trisphosphate (IP(3)) receptor, phospholipase C (PLC), or bafilomycin A1, which discharges acidic calcium stores. Melatonin-induced manganese entry provided evidence for activation of bivalent cation entry. Thus, our data suggest that melatonin evoked the elevation of platelet intracellular calcium through depletion of mitochondrial Ca(2+) stores and store-operated calcium entry (SOCE), while the action was independent of the PLC-IP(3) axis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kumari S,Dash Ddoi
10.1016/j.febslet.2011.05.067subject
Has Abstractpub_date
2011-07-21 00:00:00pages
2345-51issue
14eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00444-3journal_volume
585pub_type
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