Abstract:
:Methicillin-resistant clinical isolates of Staphylococcus aureus are intrinsically resistant to beta-lactam antibiotics in that the resistance mechanism is unrelated to the possession of beta-lactamases. We have demonstrated that a new, high-molecular-mass penicillin-binding protein (PBP) is present in these strains with a low affinity for beta-lactams and that its amount is regulated by the growth conditions. The new PBP from all strains that have been examined has an identical mobility on SDS gel electrophoresis and is the only PBP still present in an uncomplexed state with beta-lactams (and therefore the only functional PBP when these strains are grown in media containing concentrations of beta-lactam antibiotics sufficient to kill sensitive strains.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Reynolds PE,Brown DFdoi
10.1016/0014-5793(85)80036-3subject
Has Abstractpub_date
1985-11-11 00:00:00pages
28-32issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(85)80036-3journal_volume
192pub_type
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