Abstract:
:Although decapping is an important process in eukaryotic mRNA turnover, little is known about this process in plants. Here, we identified Arabidopsis thaliana decapping proteins AtDCP1 and AtDCP2 and showed that (I) AtDCP2 is an active decapping enzyme, (II) AtDCP1 interacts with itself, (III) AtDCP1 and AtDCP2 are localized to cytoplasmic foci (putative Arabidopsis processing body), and (IV) AtDCP1 and AtDCP2 are essential for post-embryonic development. Our findings provide new insights into the role of decapping-dependent mRNA turnover.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Iwasaki S,Takeda A,Motose H,Watanabe Ydoi
10.1016/j.febslet.2007.04.051subject
Has Abstractpub_date
2007-05-29 00:00:00pages
2455-9issue
13eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00455-3journal_volume
581pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The regulatory system of smooth muscle thin filaments is thought to involve a major calcium-calmodulin and actin binding protein: caldesmon. A dissective approach was used to isolate a 35 kDa C-terminal fragment of the molecule and to produce antibodies reacting against both the intact and the 15 kDa N-terminal end of...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81150-m
更新日期:1990-08-20 00:00:00
abstract::Plasma high density lipoprotein (HDL)-cholesterol levels are inversely correlated with the incidence of cardiovascular diseases. HDL is mainly assembled in the liver through the ATP-binding cassette transporter (ABCA1) pathway. In humans, plasma HDL-cholesterol levels are positively correlated with plasma adiponectin ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.09.038
更新日期:2007-10-30 00:00:00
abstract::The interactions between the polyanionic ligands phosphate and sulphate and the type II dehydroquinases from Streptomyces coelicolor and Mycobacterium tuberculosis have been characterised using a combination of structural and kinetic methods. From both approaches, it is clear that interactions are more complex in the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03346-x
更新日期:2002-10-23 00:00:00
abstract::Subcellular fractionation revealed that a significant fraction of total sphingosine kinase, the enzyme that phosphorylates sphingosine to form the bioactive lipid metabolite sphingosine-1-phosphate, resides in the nuclei of Swiss 3T3 cells, localized to both the nuclear envelope and the nucleoplasm. Platelet-derived g...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02697-7
更新日期:2001-08-10 00:00:00
abstract::We present here the purification and the analysis of the structural and functional properties of distinctin, a 5.4 kDa heterodimeric peptide with antimicrobial activity from the tree-frog Phyllomedusa distincta. This peptide was isolated from the crude extract of skin granular glands by different chromatographic steps...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02324-9
更新日期:2001-04-06 00:00:00
abstract::Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance sp...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.11.016
更新日期:2008-12-24 00:00:00
abstract::The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.03.083
更新日期:2006-05-22 00:00:00
abstract::Conserved residues of the proteolytic domain of Escherichia coli protease Lon, putative members of the classic catalytic triad (H665, H667, D676, and D743) were identified by comparison of amino acid sequences of Lon proteases. Mutant enzymes containing substitutions D676N, D743N, H665Y, and H667Y were obtained by sit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00012-x
更新日期:1998-01-30 00:00:00
abstract::Human seminal fluid contains a number of tripeptide amides with similar structures to thyrotropin releasing hormone (TRH), two of which have been identified as pGlu-Glu-Pro amide and pGlu-Phe-Pro amide. To determine whether these peptides originate in the same tissues and have the same molecular origin, TRH-immunoreac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01468-3
更新日期:1996-01-22 00:00:00
abstract::To demonstrate the interaction of calpastatin (CS) domain L (CSL) with Cav1.2 channel, we investigated the binding of CSL with various C-terminus-derived peptides at≈free, 100 nM, 10 μM, and 1mM Ca(2+) by using the GST pull-down assay method. Besides binding with the IQ motif, CSL was also found to bind with the PreIQ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.01.019
更新日期:2014-03-03 00:00:00
abstract::Mesenchymal stem cells (MSC) transplantation has been shown to decrease fibrosis in the heart; however, whether MSC directly influence the function of cardiac fibroblasts (CFB) remains unknown. MSC-conditioned medium significantly attenuated proliferation of CFB compared with CFB-conditioned medium. MSC-conditioned me...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.07.028
更新日期:2007-08-21 00:00:00
abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01015-1
更新日期:1998-09-04 00:00:00
abstract::The KlICL1 gene, encoding isocitrate lyase in Kluyveromyces lactis, is essential for ethanol utilization. Deletion analyses identified two functional promoter elements, CSRE-A and CSRE-B. Transcription is activated on ethanol, but not on glucose, glycerol or lactate. Expression depends on the KlCat8p transcription fac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.01.017
更新日期:2008-03-05 00:00:00
abstract::The regulation by insulin and carbohydrates of the gene expression of three key enzymes involved in glucose metabolism was studied in the liver of the Wistar fatty rat, a model of obese non-insulin-dependent diabetes mellitus. A high glucose or fructose diet, or insulin administration caused a similar magnitude of inc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80982-z
更新日期:1993-08-09 00:00:00
abstract::Classically, alpha-1,4-glucan synthases have been divided into two families, animal/fungal glycogen synthases (GS) and bacterial/plant starch synthases (G(S)S), according to differences in sequence, sugar donor specificity and regulatory mechanisms. Detailed sequence analysis, predicted secondary structure comparison ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(02)03313-6
更新日期:2002-09-25 00:00:00
abstract::Biotops with extreme temperatures such as deserts force animals to avoid or escape high temperatures by biochemical, behavioural or morphological adaptation. In this context we tested the resistance to heat of the oxygen carrier hemocyanin from the ancient tarantula Eurypelma californicum, which is found in arid zones...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00341-6
更新日期:1995-05-01 00:00:00
abstract::CHO SSF3 cells grow as a suspension culture in unmodified commercial medium with only low-molecular weight ingredients. Continuous serum-free culture unexpectedly induced expression of a low dihydrofolate reductase activity in the originally dhfr- CHO cells. Nevertheless, it was possible with methotrexate to induce am...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01328-8
更新日期:1995-12-27 00:00:00
abstract::Several blood-feeding organisms, including the malaria parasite detoxify haem released from host haemoglobin by conversion to the insoluble crystalline ferriprotoporphyrin IX dimer known as haemozoin. To date the mechanism of haemozoin formation has remained unknown, although lipids or proteins have been suggested to ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.043
更新日期:2006-09-18 00:00:00
abstract::The cyclic AMP phosphodiesterases type 4 (PDE4s) are expressed in a cell specific manner, with intracellular targeting directed by unique N-terminal anchor domains. All long form PDE4s are phosphorylated and activated by PKA phosphorylation within their upstream conserved region 1 (UCR1). Here, we identify and charact...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.02.004
更新日期:2015-03-12 00:00:00
abstract::We identified a yeast mutant with temperature-sensitive growth defects that were rescued by VCP expression. The mutation occurred in GPI10, which encodes a mannosyl transferase for glycosylphosphatidylinositol anchor formation in the endoplasmic reticulum, and caused a Gly469Glu substitution in Gpi10. The mutant exhib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.01.017
更新日期:2015-02-27 00:00:00
abstract::The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80187-3
更新日期:1985-01-01 00:00:00
abstract::Phosphate triester derivatives of the anti-neoplastic alkyl lyso phospholipid (ALP) have been prepared as novel potential therapeutic agents. In particular, symmetrical phosphate triesters have been prepared, using phosphorochloridate chemistry. The compounds have been fully characterised by a range of techniques, and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00959-d
更新日期:1995-09-25 00:00:00
abstract::Structural differences between platelet and endothelial cell thrombospondin (TBSP) were found in two protease-resistant domains (70 and 18 kDa). The 70 kDa fragment is involved in the binding of TBSP to fibrinogen and the 18 kDa fragment in the attachment to various cultured cells. Despite these structural differences...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80001-2
更新日期:1988-02-15 00:00:00
abstract::It is now widely recognized that the packaging of genomic DNA, together with core histones, linker histones, and other functional proteins into chromatin profoundly influences nuclear processes such as transcription, replication, DNA repair, and recombination. Whereas earlier structural studies portrayed nucleosomes (...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2004.11.030
更新日期:2005-02-07 00:00:00
abstract::Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80184-k
更新日期:1990-03-26 00:00:00
abstract::Hypo-osmotic swelling of human Intestine 407 cells leads to a significant increase of intracellular MAPKAP-kinase 2 activity and Hsp27 phosphorylation. Pre-treatment of the cells with the p38 MAP kinase inhibitor SB-203580 blocks this activation, indicating that the hypotonicity-induced activation of MAPKAP kinase 2 i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01028-9
更新日期:1996-10-21 00:00:00
abstract::Galectin-3, with a wide tissue distribution and marked developmental regulation, provides significant insights into the progression of various disease and developmental stages. Recognized by its specificity for galactose, a detailed characterization of its sugar binding ability has been investigated by isothermal titr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02586-8
更新日期:2001-06-29 00:00:00
abstract::Peptide T, an octapeptide of sequence ASTTTNYT that binds to human T cells, was studied as a zwitterion in DMSOd6 solution by means of proton NMR spectroscopy at 500 MHz. The unusual dispersion of the resonances of residues of the same type (T) makes it possible to assign all resonances to specific residues by means o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80723-3
更新日期:1988-04-11 00:00:00
abstract::The possibility that the mutant mouse wasted (wst/wst) may serve as an animal model for studies of severe combined immunodeficiency disease (SCID) and the role of adenosine deaminase (ADA, EC 3.5.4.4) in adenosine metabolism were investigated. The specific activity of ADA in wst/wst compared with control mice was sign...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)81063-8
更新日期:1986-11-24 00:00:00
abstract::A human Cu,Zn-superoxide dismutase (Cu,Zn-SOD) gene was fused with a gene fragment encoding the nine amino acid transactivator of transcription (Tat) protein transduction domain (RKKRRQRRR) of HIV-1 in a bacterial expression vector to produce a genetic in-frame Tat-SOD fusion protein. The expressed and purified Tat-SO...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02215-8
更新日期:2000-11-24 00:00:00