Abstract:
:The yeast PCK1 gene coding for phosphoenolpyruvate carboxykinase (PEPCK) was isolated by functional complementation of pck1 strains from S. cerevisiae. Only one copy of the gene was found per haploid yeast genome. An RNA of about 2 kb which hybridized with a DNA probe internal to the PCK1 gene was found only in cells growing in non-fermentable carbon sources. Yeast strains carrying multiple copies of the PCK1 gene showed normal catabolite repression of PEPCK except those carrying the shortest insertion complementing the mutation (2.2 kb) that presented an altered kinetics of derepression. Catabolite inactivation was decreased in strains transformed with multicopy plasmids carrying the PCK1 gene.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Valdés-Hevia MD,de la Guerra R,Gancedo Cdoi
10.1016/0014-5793(89)81682-5subject
Has Abstractpub_date
1989-12-04 00:00:00pages
313-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(89)81682-5journal_volume
258pub_type
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