Estimation of flux control coefficients from inhibitor titrations by non-linear regression.

abstract：:Escherichia coli outer membrane protease OmpT has been characterised as a serine protease based on its inhibitor profile, but serine protease consensus sequences are absent. By site-directed mutagenesis we substituted all conserved serines and histidines. Substitution of His(101) and His(212) by Ala, Asn or Gln result...

journal_title：FEBS letters

authors： Kramer RA,Dekker N,Egmond MR

更新日期：2000-02-25 00:00:00

abstract：:Using the patch-clamp technique, we demonstrate here the opening of K+ channels evoked by the actively transported amino acid L-alanine in isolated Necturus enterocytes. These channels had a conductance of about 30 pS and their activation was dependent on transmembrane electrical potential and cytosolic Ca2+. ...

journal_title：FEBS letters

authors： Sheppard DN,Giraldez F,Sepúlveda FV

更新日期：1988-07-18 00:00:00

abstract：:We report for the first time the identification of 25 microRNAs from tissues originating from chicken embryo and adult chicken. Most of the cloned microRNAs are expressed in both adult chickens and chicken embryos. Fourteen were identified without any prior prediction. One microRNA, miR-757, is thought to be chicken-s...

journal_title：FEBS letters

authors： Xu H,Wang X,Du Z,Li N

更新日期：2006-06-26 00:00:00

abstract：:A novel protein that binds specifically to the intracellular domain of the p55 tumor necrosis factor (TNF) receptor was cloned by two-hybrid screening of a HeLa cell cDNA library. Data bank searches revealed high sequence similarity of the protein (55.11) to yeast, nematode and plant proteins, whose functions are yet ...

journal_title：FEBS letters

authors： Boldin MP,Mett IL,Wallach D

更新日期：1995-06-19 00:00:00

abstract：:At present, little is known about signal transduction mechanisms in schistosomes, which cause the disease of schistosomiasis. The mitogen-activated protein kinase (MAPK) signaling pathways, which are evolutionarily conserved from yeast to Homo sapiens, play key roles in multiple cellular processes. Here, we reconstruc...

journal_title：FEBS letters

authors： Wang L,Yang Z,Li Y,Yu F,Brindley PJ,McManus DP,Wei D,Han Z,Feng Z,Li Y,Hu W

更新日期：2006-06-26 00:00:00

abstract：:We have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylinositol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in ...

journal_title：FEBS letters

authors： van den Akker WM,Westerman J,Gadella TW Jr,Wirtz KW,Snoek GT

更新日期：1990-12-10 00:00:00

abstract：:Thymidine kinase in chick embryo retina reaches its highest values on the 8-10th day of development, then declines reaching the lowest value at hatching. The rate of DNA synthesis essentially follows this activity while, in contrast, nucleoside phosphotransferase increases progressively during development. Glucocortic...

journal_title：FEBS letters

authors： Tesoriere G,Tesoriere L,Giuliano M,Vento R

更新日期：1983-11-28 00:00:00

abstract：:Leishmania major promastigotes are parasites endowed with a plasma membrane electrogenic H+ pump and anionic channels. These systems have been thought to contribute to pH homeostasis of parasites and environmental adaptation by mediating extrusion of protons which are either generated metabolically or result from exog...

journal_title：FEBS letters

authors： Vieira L,Cabantchik ZI

更新日期：1995-03-13 00:00:00

abstract：:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and als...

journal_title：FEBS letters

authors： Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela K

更新日期：2006-03-06 00:00:00

abstract：:miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-α treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that s...

journal_title：FEBS letters

authors： Mi W,Shi Q,Chen X,Wu T,Huang H

更新日期：2016-02-01 00:00:00

abstract：:In the mouse macrophage-like cell line RAW 264, vacuolar-type (H(+))-ATPase (V-ATPase) inhibitors, bafilomycin A(1) and concanamycin A, increased the level of cyclooxygenase (COX)-2 protein and its mRNA. The V-ATPase inhibitor-induced expression of COX-2 was suppressed by inhibitors of c-jun N-terminal kinase (JNK) an...

journal_title：FEBS letters

authors： Kamachi F,Yanai M,Ban HS,Ishihara K,Hong J,Ohuchi K,Hirasawa N

更新日期：2007-10-02 00:00:00

abstract：:Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

journal_title：FEBS letters

authors： Matzura H

更新日期：1970-01-15 00:00:00

abstract：:The RNA helicase Rok1 plays a role in rRNA processing and in control of cell cycle progression in Saccharomyces cerevisiae. We identified two upstream open reading frames (uORFs) within the ROK1 5' untranslated region, which inhibited Rok1 translation. Mutating uATG to uAAG or generation of a premature stop codon in t...

journal_title：FEBS letters

authors： Jeon S,Kim J

更新日期：2010-11-19 00:00:00

abstract：:The isocitrate lyase-encoding gene AgICL1 from the filamentous hemiascomycete Ashbya gossypii was isolated by heterologous complementation of a Saccharomyces cerevisiae icl1d mutant. The open reading frame of 1680 bp encoded a protein of 560 amino acids with a calculated molecular weight of 62584. Disruption of the Ag...

journal_title：FEBS letters

authors： Maeting I,Schmidt G,Sahm H,Revuelta JL,Stierhof YD,Stahmann KP

更新日期：1999-02-05 00:00:00

abstract：:A single copy of a glucocorticoid-responsive element (GRE) is sufficient in mediating the combinatorial response of a promoter to both glucocorticoids and insulin in HepG2 cells. This requires the presence of active glucocorticoid receptor (GR) since the response is significantly inhibited by the anti-glucocorticoid R...

journal_title：FEBS letters

authors： Georgakopoulos A,Tsawdaroglou N

更新日期：1996-03-04 00:00:00

abstract：:Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

journal_title：FEBS letters

authors： Thomson AJ

更新日期：1991-07-22 00:00:00

abstract：:Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

journal_title：FEBS letters

authors： Basu A,Haldar S

更新日期：2003-03-13 00:00:00

abstract：:The rat synapse associated protein SAP90 is a member of a superfamily of potential guanylate kinases localized at cell-cell contact sites. This superfamily includes the synapse associated protein SAP97, a close relative of SAP90, the Drosophila tumor suppressor gene product dlg-Ap, the mammalian zonula occludens prote...

journal_title：FEBS letters

authors： Kistner U,Garner CC,Linial M

更新日期：1995-02-13 00:00:00

abstract：:Human complement component C8gamma is an unusual complement factor since it shows no homology to other complement proteins but is a member of the lipocalin superfamily. So far, it has been found exclusively in plasma, covalently linked to C8alpha by disulfide bridging. We have used dot blot and Northern blot analyses ...

journal_title：FEBS letters

authors： Trojer P,Wojnar P,Merschak P,Redl B

更新日期：1999-03-12 00:00:00

abstract：:Cells of Saccharomyces cerevisiae were loaded with indo-1, by incubation in a medium of pH 4.5, which contained penta-potassium indo-1. Cells were then washed and resuspended in a buffer of pH 4.0. The emission fluorescence spectra were recorded between 390 and 500 nm (excitation at 355 nm) and the autofluorescent spe...

journal_title：FEBS letters

authors： Halachmi D,Eilam Y

更新日期：1989-10-09 00:00:00

abstract：:Lipoprotein MtsA is a critical component of MtsABC responsible for iron binding and transport in the Gram-positive bacterium Streptococcus pyogenes. The present collective experimental data establish that Fe(2+) is the primary binding ion for MtsA under optimal physiologically relevant conditions. The binding affiniti...

journal_title：FEBS letters

authors： Sun X,Ge R,Chiu JF,Sun H,He QY

更新日期：2008-04-16 00:00:00

abstract：:RCR cells are NRK clones in which Raf-1 production is blocked by the expression of an antisense RNA, and consequently they are refractory to transformation by various oncogenes. In RCR cells, MAP kinases (ERK1 and ERK2) were activated to an extent and in a time course similar to those of the original NRK cells, irresp...

journal_title：FEBS letters

authors： Kizaka-Kondoh S,Okayama H

更新日期：1993-12-27 00:00:00

abstract：:In the unfolded protein response (UPR), Ire1 activates Hac1 to coordinate the transcription of hundreds of genes to mitigate ER stress. Recent work in Caenorhabditis elegans suggests that oxidative stress inhibits this canonical Ire1 signalling pathway, activating instead an antioxidant stress response. We sought to d...

journal_title：FEBS letters

authors： Guerra-Moreno A,Ang J,Welsch H,Jochem M,Hanna J

更新日期：2019-05-01 00:00:00

abstract：:The cholecystokinin (CCK) receptor in purified plasma membranes prepared from mouse pancreatic acini had a binding affinity of 1.8 nM, an acid pH optimum between 6.0 and 6.5, and an analog specificity of CCK8 greater than CCK33 greater than desulphated CCK8 greater than CCK4. Binding of CCK to its receptor was abolish...

journal_title：FEBS letters

authors： Sakamoto C,Williams JA,Wong KY,Goldfine ID

更新日期：1983-01-10 00:00:00

abstract：:Ribonuclease T1 can be unfolded and refolded without forming noticeable amounts of aggregates allowing to characterise the dimensions of a protein in different denatured states in terms of the Stokes radius RS. Upon thermal unfolding RS increases from 1.74 nm at 20 degrees C to 2.14 nm at 60 degrees C. By contrast, RS...

journal_title：FEBS letters

authors： Gast K,Zirwer D,Damaschun H,Hahn U,Müller-Frohne M,Wirth M,Damaschun G

更新日期：1997-02-24 00:00:00

abstract：:Copper(II) was substituted for manganese(III) in Bacillus stearothermophilus Mn-superoxide dismutase. The (EPR) spectrum of the Cu(II) is distinctly rhombic, overlapping that of Cu(II) replacing zinc in copper/zinc superoxide dismutase. The copper-cyanide complex of the bacterial enzyme gives an EPR spectrum nearly id...

journal_title：FEBS letters

authors： Bannister JV,Desideri A,Rotilio G

更新日期：1985-08-19 00:00:00

abstract：:LipL and Cpr19 are nonheme, mononuclear Fe(II)-dependent, α-ketoglutarate (αKG):UMP oxygenases that catalyze the formation of CO2 , succinate, phosphate, and uridine-5'-aldehyde, the last of which is a biosynthetic precursor for several nucleoside antibiotics that inhibit bacterial translocase I (MraY). To better unde...

journal_title：FEBS letters

authors： Goswami A,Liu X,Cai W,Wyche TP,Bugni TS,Meurillon M,Peyrottes S,Perigaud C,Nonaka K,Rohr J,Van Lanen SG

更新日期：2017-02-01 00:00:00

abstract：:The thermal unfolding of the myosin subfragment 1 (S1) in its stable complex with ADP and beryllium fluoride (S1.ADP.BeF3-) was studied by differential scanning calorimetry. It has been shown that the structure of the S1 molecule in the S1.ADP.BeF3- complex is similar to that of S1 in its complex with ADP and orthovan...

journal_title：FEBS letters

authors： Bobkov AA,Khvorov NV,Golitsina NL,Levitsky DI

更新日期：1993-10-11 00:00:00

abstract：:Several lines of evidence indicate that selenoproteins mainly act as cellular antioxidants. Here, we test this idea comparing the sensitivity to oxidative stress (paraquat and hydrogen peroxide) between wild type and heterozygous flies for the selenophosphate synthetase selD(ptuf) mutation. Whereas under normal labora...

journal_title：FEBS letters

authors： Morey M,Serras F,Corominas M

更新日期：2003-01-16 00:00:00

abstract：:Methylation of cytosine-residues in the sequence CpG affects the expression of many genes and generally correlates with reduced transcription. The ribosomal genes of Xenopus laevis were among the first genes to be studied with respect to their DNA methylation, and a loss of methylation during embryonic development cor...

journal_title：FEBS letters

authors： Labhart P

更新日期：1994-12-19 00:00:00