Abstract:
:Human complement component C8gamma is an unusual complement factor since it shows no homology to other complement proteins but is a member of the lipocalin superfamily. So far, it has been found exclusively in plasma, covalently linked to C8alpha by disulfide bridging. We have used dot blot and Northern blot analyses of a large number of different human tissues to survey systematically the expression pattern of C8gamma. Our experiments clearly showed that besides in liver, this gene is also expressed in fetal and adult kidney. Renal expression of C8gamma is not dependent on C8alpha expression, since we could not detect C8alpha expression in kidney. Thus its physiological function is not restricted to a specific action in association with complement components. As a prerequisite for further characterization of the structure and binding activities of the uncomplexed C8gamma, we have expressed the encoding cDNA in Escherichia coli. To increase the probability for proper folding of the characteristic intramolecular disulfide bridge the recombinant protein was produced by secretion to the periplasm.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Trojer P,Wojnar P,Merschak P,Redl Bdoi
10.1016/s0014-5793(99)00228-8keywords:
subject
Has Abstractpub_date
1999-03-12 00:00:00pages
243-6issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00228-8journal_volume
446pub_type
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