Abstract:
:Interaction of lectins with cell surface determinants may alter membrane properties. Using trypsinized rabbit erythrocytes as model we tested the capacity of an endogenous lectin in this respect. Galectin-1 is a member of an adhesion/growth-regulatory family known to interact for example with ganglioside GM(1) and also the hydrophobic tail of oncogenic H-Ras. Assays on membrane fluidity and osmofragility detect galectin-1's capacity to increase the parameters. Moreover, it increases susceptibility of erythrocytes to radical damage. These observations indicate the potential of this endogenous lectin to affect membrane properties beyond the immediate interaction with cell surface epitopes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Gupta RK,Pande AH,Gulla KC,Gabius HJ,Hajela Kdoi
10.1016/j.febslet.2006.02.006keywords:
subject
Has Abstractpub_date
2006-03-06 00:00:00pages
1691-5issue
6eissn
0014-5793issn
1873-3468pii
S0014-5793(06)00193-1journal_volume
580pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The biochemical properties of the p51 subunit of HIV-1 reverse transcriptase (RT) were studied in order to understand its role in the heterodimeric form p66/p51 found in virions. A recombinant form of RT, p51/p51, expressed in yeast, was purified and characterized. The enzyme was affinity labeled using a 5' modified o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80202-r
更新日期:1992-04-13 00:00:00
abstract::The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.07.038
更新日期:2015-09-14 00:00:00
abstract::Lipoic acid is a coenzyme essential to the activity of enzymes such as pyruvate dehydrogenase, which play important roles in central metabolism. However, neither the enzymes responsible for biosynthesis nor the biosynthetic event of lipoic acid has been reported in mammalian cells. In this study, a mouse mLIP1 cDNA fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02469-3
更新日期:2001-06-01 00:00:00
abstract::The effects of metal cations on the activation of recombinant human SK4 (also known as hIK1 or hKCa4) channels, expressed in HEK 293 cells, were tested using patch clamp recording. Of the nine metals tested, cobalt, iron, magnesium, and zinc did not activate the SK4 channels when applied, at concentrations up to 100 m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00194-5
更新日期:1999-03-05 00:00:00
abstract::Pioneer crystallographic studies of the isolated 30S ribosomal subunit provided the first structural insights into the decoding process. Recently, new crystallographic data on full 70S ribosomes with mRNA and tRNAs have shown that the formation of the tight decoding centre is ensured by conformational rearrangement of...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2013.05.009
更新日期:2013-06-27 00:00:00
abstract::Early results showed the emanation of chromatin fibers from mitotic chromosomes and nuclei swollen with water. In contrast, under metaphase ionic conditions, it was found that chromatin from mitotic chromosomes is planar and forms multilayered plates. Here, we show that in buffers containing interphase cation concentr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13370
更新日期:2019-04-01 00:00:00
abstract::Amino acid sequencing of a 48/46 kDa glycoprotein from human plantar callus, recognised by antisera raised against the desmosomal cadherins DGII/III, has revealed N-terminal homology to the DNA-derived sequence of human and bovine DGII/III. However, a tryptic fragment has homology only with a bovine clone. We propose ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80929-w
更新日期:1991-07-29 00:00:00
abstract::We examined the effects of histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values. Using a monoclonal antibody specific for hen egg-white lysozyme and three mutant antibodies that contained a histidine residue,...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80745-3
更新日期:1992-08-31 00:00:00
abstract::Circular dichroism (CD) spectra have been obtained from several variants of green fluorescent protein: blue fluorescent protein (BFP), enhanced cyan fluorescent protein (CFP), enhanced green fluorescent protein (GFP), enhanced yellow fluorescent protein (YFP), all from Aequorea victoria, and the red fluorescent protei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02808-9
更新日期:2002-06-19 00:00:00
abstract::We recently purified two closely related 33 kDa proteins from rat hepatic cytosol, designated bile acid binder I and II, which selectively bind bile acids with comparable affinity as glutathione S-transferase B. This work has now been extended to human liver in which we have identified a similar cytosolic binding acti...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80975-8
更新日期:1984-11-05 00:00:00
abstract::Increased expression of low voltage-activated, T-type Ca(2+) channels has been correlated with a variety of cellular events including cell proliferation and cell cycle kinetics. The recent cloning of three genes encoding T-type alpha(1) subunits, alpha(1G), alpha(1H) and alpha(1I), now allows direct assessment of thei...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01832-9
更新日期:2000-07-28 00:00:00
abstract::Oxygen is an essential element in the survival of complex organisms, however the level of oxygen, low or high, can be a source of stress depending on the biological context. Low levels of oxygen in tissues (hypoxia) can be the consequence of a number of pathophysiological conditions including ischemic disorders and ca...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2007.06.018
更新日期:2007-07-31 00:00:00
abstract::DNA complementary to mRNA extracted from the thyroid glands of patients suffering from medullary carcinoma of the thyroid (MCT), a calcitonin-producing tumour, was inserted in the Pst site of pBR 322 by G-C tailing. The recombinant plasmids were used to transform Escherichia coli DP 50. Ampicillin-resistant clones wer...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80839-x
更新日期:1984-02-13 00:00:00
abstract::Olfactory cilia contain cyclic nucleotide-gated and Ca2+-dependent Cl- conductances that underlie excitatory chemotransduction, and a Ca2+-dependent K+ (KCa) conductance, apparently involved in inhibitory transduction. Previous single-channel patch-clamp studies on olfactory cilia revealed four different KCas, with di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.079
更新日期:2005-03-14 00:00:00
abstract::Amyloid precursor protein (APP) gene expression was investigated in primary cultures of neurons, astrocytes, microglial cells and oligodendrocytes. Neurons from various rat brain regions, as well as oligodendrocytes, contained RNA encoding APP695, while astrocytes and microglial cells expressed high levels of RNAs for...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80706-m
更新日期:1992-08-03 00:00:00
abstract::The binding of phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross-linked profilin:beta-actin. The result is in agreement with observations made with several mutant profilins and provides strong ev...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02913-7
更新日期:2002-07-03 00:00:00
abstract::Folding of nascent chains resembles the decoding of spoken language in that information is emitted as a unidirectional, one-dimensional string of elements, with higher structures and long-distance interactions emerging with time. Applying a "pseudolinguistic' analysis of structure to a set of all 36 possible six-stran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00727-2
更新日期:1996-07-29 00:00:00
abstract::Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or Rho...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.03.044
更新日期:2012-05-07 00:00:00
abstract::The cloning and analysis of a cDNA clone encoding the soybean metalloproteinase obtained by polymerase chain reaction (PCR) and the rapid amplification of cDNA ends (RACE) reaction are described. The cDNA was constructed from poly(A)+ RNA isolated from 15-17 day old leaves. The deduced amino acid sequence of the cDNA ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00141-5
更新日期:1997-03-10 00:00:00
abstract::Disulfide bridges were introduced into CrylAa, a Bacillus thuringiensis lepidopteran toxin, to stabilize different protein domains including domain I alpha-helical regions thought to be involved in membrane integration and permeation. Bridged mutants could not form functional ion channels in lipid bilayers in the oxid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00626-1
更新日期:1997-06-30 00:00:00
abstract::Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00295-6
更新日期:1998-04-03 00:00:00
abstract::Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.10.013
更新日期:2014-11-28 00:00:00
abstract::A Trp-free alphaA-crystallin mutant (W9F) was prepared by site-directed mutation. This mutant appears to be identical to the wild-type in terms of conformation (secondary and tertiary structures). W9F was labeled with a sulfhydryl-specific fluorescent probe, 2-(4'-maleimidylanilino) naphthalene-6-sulfonate (MIANS), an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00707-8
更新日期:1998-07-03 00:00:00
abstract::The possibility that the phospholipid-N-methyltransferases from yeast are capable of acting upon a phospholipid substrate, localized in a different membrane than in which the enzymes reside ('trans-catalysis' hypothesis), was investigated using cho2 and opi3 gene disruptant strains, which are defective in phosphatidyl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02298-6
更新日期:2002-02-27 00:00:00
abstract::The accumulation of protoporphyrin IX (Proto IX) in light-sensitive mutants of Escherichia coli was detected by spectrofluorimetry. Fluorescence emission and excitation spectra were recorded from extracts of bacterial cells. Proto IX clearly accumulated in cells with mutations in the visA (hemH) gene but not in the wi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81341-i
更新日期:1992-10-05 00:00:00
abstract::Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.063
更新日期:2010-01-04 00:00:00
abstract::Primary cultures of rat dorsal root ganglionic (DRG) cells were stained with isoform-specific antibodies against non-muscle myosin II. Antibodies against the brain type myosin (MIIB) stained the peripheries of growth cones and non-neuronal cells. Double staining of the cells with the anti-myosin antibodies and rhodami...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81374-u
更新日期:1992-10-19 00:00:00
abstract::Expression of androgen receptor (AR) in prostate epithelial cells is thought to regulate cell proliferation, differentiation, and survival. However, the molecular mechanisms remain unclear. We report that re-expression of AR in PC-3 human prostate cancer cell line resulted in upregulation of IFI16 protein, a negative ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.02.015
更新日期:2006-03-06 00:00:00
abstract::Structural integrity may be needed for the glutathione-linked peroxidase activity of human ceruloplasmin. Intact human ceruloplasmin has a potent peroxidase property to decompose H2O2 in the presence of reduced glutathione. However, the fragment of approximately 116000 Da produced by proteolytic degradation had less t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01253-8
更新日期:1998-10-23 00:00:00
abstract::The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then ov...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80171-4
更新日期:1984-01-09 00:00:00