Abstract:
:We have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylinositol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in phosphatidylinositol transfer activity and a 10-fold higher affinity for this phospholipid. The possibility that the 38 kDa protein is a precursor of the phosphatidylinositol transfer protein is discussed.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
van den Akker WM,Westerman J,Gadella TW Jr,Wirtz KW,Snoek GTdoi
10.1016/0014-5793(90)80523-lsubject
Has Abstractpub_date
1990-12-10 00:00:00pages
123-6issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)80523-Ljournal_volume
276pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Glycogen synthase kinase-5 (casein kinase-II) phosphorylates glycogen synthase on a serine termed site 5. This residue is just C-terminal to the 3 serines phosphorylated by glycogen synthase kinase-3, which are critical for the hormonal regulation of glycogen synthase in vivo. Although phosphorylation of site 5 does n...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(82)81332-x
更新日期:1982-12-13 00:00:00
abstract::F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12521
更新日期:2017-01-01 00:00:00
abstract::Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00692-9
更新日期:1998-07-03 00:00:00
abstract::Leishmania internalize hemoglobin (Hb) via a specific receptor (HbR) for their survival. To identify the Hb-binding domain of HbR, we cloned and expressed several truncated proteins of HbR and determined their ability to bind Hb. Our findings reveal that 90% of Hb-binding activity is retained in HbR41-80 in comparison...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.14027
更新日期:2020-12-14 00:00:00
abstract::A Bacillus subtilis strain deficient in seven extracellular proteases was used to produce human mast cell chymase and is a viable expression system for serine proteases and other classes of proteins. Chymase is produced at 0.3-0.5 mg/l and is purified by three chromatography steps. Two crystal forms of PMSF-treated ch...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00962-9
更新日期:1997-08-25 00:00:00
abstract::There is a paucity of information on the unique components that pathogens use to form respiratory chains. It is not known why mycobacteria encode multiple succinate dehydrogenases (SDHs) to perform menaquinone-linked succinate oxidation, a thermodynamically unfavorable reaction (ΔG° = +21 kJ·mol-1 ). In other bacteria...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13330
更新日期:2019-03-01 00:00:00
abstract::The ubiquitous transcription factor Sp1 has been implicated in the mechanism which maintains CpG islands methylation-free. Plasmids containing GC boxes (Sp1 sites) were in vitro methylated at every CpG dinucleotide. After stable introduction into F9 embryonal carcinoma cells, we analysed the methylation of the sequenc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00830-3
更新日期:1995-08-21 00:00:00
abstract::We have used a panel of monoclonal antibodies in a study of the expression of multiple tubulins in Physarum polycephalum. Three anti-beta-tubulin monoclonal antibodies, DM1B, DM3B3 and KMX-1 all reacted with the beta 1-tubulin isotypes expressed in both myxamoebae and plasmodia. However, these antibodies showed a spec...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81244-8
更新日期:1985-08-05 00:00:00
abstract::The CA microsatellite repeat marker, D7S522, is located at the center of a approximately 1000 kb smallest common deleted region that is lost in many forms of human cancer. It has been proposed that a putative tumor suppressor gene lies in close proximity to D7S522, within this smallest common deleted region. However, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00365-8
更新日期:1999-04-09 00:00:00
abstract::Reg, first isolated from a rat regenerating islet cDNA library, is expressed in regenerating islet beta-cells. Recently, it has been revealed that Reg and Reg-related genes constitute a multigene family, the Reg family. In human, the four REG family genes, i.e., REG 1 alpha, REG 1 beta, REG-related sequence (RS) and H...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01381-4
更新日期:1995-12-27 00:00:00
abstract::Apoptosis repressor with caspase recruitment domain is expressed at high levels in brain and myogenic tissues, consistent with a role to inhibit apoptosis in the terminally differentiated cells. Expression of ARC in cancers is not known. In this study, we reported that ARC was highly expressed in various non-myogenic ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.03.040
更新日期:2005-04-25 00:00:00
abstract::Mona is an SH3 and SH2 domain-containing adapter molecule that is induced during monocytic differentiation. Here we have first shown that M-CSFR is the major Mona partner in M-CSF signaling, the interaction being mediated through tyrosine 697 of the receptor. Next we asked whether Mona expression would alter the Ras/M...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01906-2
更新日期:2000-09-01 00:00:00
abstract::We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol poly...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00880-6
更新日期:1997-08-11 00:00:00
abstract::Here we report the first crystal structure of a protein, AmyA, a secretory alpha-amylase isolated from Halothermothrix orenii, which is both halophilic and thermophilic. The crystal structure was determined at 1.6 A resolution. AmyA lacks the conserved acidic surface, which is considered essential for protein stabilit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.04.017
更新日期:2006-05-15 00:00:00
abstract::Nitrogen dioxide (NO2.) is often present in inhaled air and may be generated in vivo from nitric oxide. Exposure of human blood plasma to NO2. caused rapid losses of ascorbic acid, uric acid and protein thiol groups, as well as lipid peroxidation and depletions of alpha-tocopherol, bilirubin and ubiquinol-10. No incre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81185-o
更新日期:1992-11-16 00:00:00
abstract::Soluble factors derived from human CD8+ T-lymphocytes inhibit HIV-1 replication by suppressing transcription from the viral long terminal repeat (LTR), an effect shown to be mediated in part by an NFAT-1 enhancer sequence. We show here that the CD8+ derived beta-chemokines, RANTES, MIP1-alpha, and MIP-1beta, known sup...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00654-6
更新日期:1997-06-30 00:00:00
abstract::The changes in phosphoproteins purified with the affinity peptide p9CKShs1 were analyzed from extracts of regenerating rat livers in order to define some G1 and G1/S regulations characteristic of mature hepatocytes stimulated to proliferate. We observed a 47 kDa phosphoprotein that occurred first at the end of G1 befo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00629-8
更新日期:1999-06-11 00:00:00
abstract::Mutations in PTEN-induced putative kinase 1 (PINK1) cause recessive form of Parkinson's disease (PD). PINK1 acts upstream of parkin, regulating mitochondrial integrity and functions. Here, we show that PINK1 in combination with parkin results in the perinuclear mitochondrial aggregation followed by their elimination. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.016
更新日期:2010-03-19 00:00:00
abstract::Previous studies using retinal analogs (Wada, A., Sakai, M., Kinumi, T., Tsujimoto, K., Yamauchi, M. and Ito, M. (1994) J. Org. Chem. 59, 6992-6997; Wada, A., Sakai, M., Imamoto, Y., Shichida., Y., Yoshizawa, T. and Ito, M. (1993) Chem. Pharm. Bull. 41, 793-795) revealed that both retinochrome and visual pigments shar...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01004-7
更新日期:1998-09-04 00:00:00
abstract::Msn2 and Msn4 transcription factors activate expression of stress-responsive element (STRE) controlled genes in response to various stresses triggering the environmental stress response in Saccharomyces cerevisiae. Although high hydrostatic pressure is known to induce gene expression modification in yeast, the transcr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.007
更新日期:2006-11-13 00:00:00
abstract::LncRNAs have a critical role in the regulation of cellular processes such as cancer progression and metastasis. In the present study, we confirmed that TUG1 was overexpressed in bladder cancer tissues and established cell lines. Knockdown of TUG1 inhibited bladder cancer cell metastasis both in vitro and in vivo. Furt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.020
更新日期:2015-10-07 00:00:00
abstract::A procedure for isolation of two iso-plastocyanins from parsley has been described here. Three consecutive chromatographic steps on DE-52-Whatman cellulose were applied for isolation of two total plastocyanin (PC) fractions, oxidized [PC(II)] and reduced [PC(I)]. By chromatofocusing of PC(II) on Polybuffer exchanger 7...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80904-w
更新日期:1990-06-04 00:00:00
abstract::Glycogen synthase from human and bovine polymorphonuclear leukocytes was purified to homogeneity. Rabbit antisera were raised against the two glycogen synthases and used for immunochemical analysis. Western blotting analysis showed that the subunit of glycogen synthase in crude homogenates of human and bovine leukocyt...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80653-7
更新日期:1988-03-28 00:00:00
abstract::The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03200-8
更新日期:2001-12-14 00:00:00
abstract::Antiparaoxon immune sera were employed in a new immunoassay based on competition between acetylcholinesterase and antibodies for the binding of paraoxon. Unlike radioimmunoassay, the new assay described herein can be extended to predict the feasibility of antibodies to confer in vivo protection of acetylcholinesterase...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81079-6
更新日期:1985-01-28 00:00:00
abstract::Circadian clocks are endogenous timing systems orchestrating the daily regulation of a huge variety of physiological, metabolic and behavioral processes. These clocks are important for health - in mammals, their disruption leads to a diverse number of pathologies. While genetic and biochemical approaches largely uncov...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2015.04.059
更新日期:2015-06-22 00:00:00
abstract::Autophagy in the yeast is similar to that in mammalian cells. A mutant designated as apg1 (autophagy) defective in accumulation of autophagic bodies in the vacuoles was isolated by selection using a light microscope from a mutagenized proteinase-deficient strain. In the apg1 strain, which has normal vacuolar proteinas...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80398-e
更新日期:1993-10-25 00:00:00
abstract::The binding of phosphatidylinositol(4,5)-bisphosphate (PI(4,5)P(2)) to profilin at a region distinct from the actin interaction surface is demonstrated by experiments with covalently cross-linked profilin:beta-actin. The result is in agreement with observations made with several mutant profilins and provides strong ev...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02913-7
更新日期:2002-07-03 00:00:00
abstract::The complete sequence of a 94 amino acid human seminal plasma polypeptide exhibiting inhibin-like activity is presented. This molecule, called beta-inhibin, selectively and specifically suppresses the release of pituitary FSH in vivo as well as in vitro. It does not affect the secretion of LH. Such a novel acidic prot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80766-8
更新日期:1984-10-01 00:00:00
abstract::DNA chip technology was used in an attempt to identify target genes responsible for apoptosis induced by etoposide, a p53 activating topoisomerase II inhibitor used clinically as an antitumor agent. 62 Individual mRNAs whose mass changed significantly were identified after screening oligonucleotide arrays capable of d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00136-2
更新日期:1999-02-26 00:00:00