Production of crystallizable human chymase from a Bacillus subtilis system.

abstract：:Microsomal membranes from the slow-growing Morris hepatoma 9618A catalyze, in the presence of t-butyl hydroperoxide, lower rates of lipid peroxidation than rat liver microsomes. The cytochrome P-450 content of hepatoma microsomes is about 40% that of the liver. SKF 525-A, an inhibitor of mixed-function oxidase, produc...

journal_title：FEBS letters

authors： Borrello S,Galeotti T,Palombini G,Minotti G

更新日期：1986-12-15 00:00:00

abstract：:Using specific antisera, the expression of the G protein alpha subunits, G8, G(i1), G(i2), G(i3) and G0, were determined in 3T3-F442A cells during their differentiation to adipocytes in a hormonally defined medium. Differentiation caused distinct increases in the expression of two Gs isoforms and decreases in the expr...

journal_title：FEBS letters

authors： Kilgour E,Anderson NG

更新日期：1993-08-16 00:00:00

abstract：:Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...

journal_title：FEBS letters

authors： Austin RC,Rachubinski RA,Blajchman MA

更新日期：1991-03-25 00:00:00

abstract：:The involvement of reactive oxygen species in Ca(2+)-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2, 4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Kowaltowski AJ,Vercesi AE,Rhee SG,Netto LE

更新日期：2000-05-12 00:00:00

abstract：:In order to get a better insight into the function of amino acid residues located in the second transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, all exon 18 mutations found in cystic fibrosis (CF) patients were characterized at the protein and at the electrophysiological ...

journal_title：FEBS letters

authors： Vankeerberghen A,Wei L,Teng H,Jaspers M,Cassiman JJ,Nilius B,Cuppens H

更新日期：1998-10-16 00:00:00

abstract：:Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...

journal_title：FEBS letters

authors： Fauconnier ML,Williams TD,Marlier M,Welti R

更新日期：2003-03-13 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) and Rho-kinase are related. An important function of Rho-kinase is to phosphorylate the myosin-binding subunit of myosin phosphatase (MYPT1) and inhibit phosphatase activity. Experiments were carried out to determine if DMPK could function similarly. MYPT1 was phosphorylated by...

journal_title：FEBS letters

authors： Murányi A,Zhang R,Liu F,Hirano K,Ito M,Epstein HF,Hartshorne DJ

更新日期：2001-03-30 00:00:00

abstract：:We examined the role of p38 mitogen-activated protein (MAP) kinase in the tumor necrosis factor alpha (TNF-alpha)- or interleukin-1beta (IL-1beta)-induced production of interleukin-6 (IL-6) and interleukin-8 (IL-8) in fresh rheumatoid synovial fibroblast (RSF) cultures concomitantly with the induction of p38 MAP kinas...

journal_title：FEBS letters

authors： Suzuki M,Tetsuka T,Yoshida S,Watanabe N,Kobayashi M,Matsui N,Okamoto T

更新日期：2000-01-07 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:For studying the role of Ser132 in the putative catalytic site of human lipoprotein lipase (LPL), mutant LPL cDNAs expressing LPLs with amino acid substitutions of Gly or Asn for Ser132 were obtained by site-directed mutagenesis, and were expressed in COS-1 cells. Considerable amounts of LPL enzyme protein mass were d...

journal_title：FEBS letters

authors： Tashiro J,Kobayashi J,Shirai K,Saito Y,Fukamachi I,Hashimoto H,Nishida T,Shibui T,Morimoto Y,Yoshida S

更新日期：1992-02-17 00:00:00

abstract：:This work describes the molecular cloning of a variant isoform of the low-affinity levocabastine-sensitive neurotensin receptor isolated from mouse brain. Although the corresponding mRNA encodes for a 282 amino acid protein unable to bind neurotensin after transient transfection in COS-7 cells, this non-functional neu...

journal_title：FEBS letters

authors： Botto JM,Sarret P,Vincent JP,Mazella J

更新日期：1997-01-03 00:00:00

abstract：:Fas/FasL interactions have been proposed as a potentially important mechanism mediating beta-cell death in type 1 diabetes. Recent investigations suggest RNA interference, afforded by small interfering RNAs (siRNA), can provide specific and robust gene silencing in mammalian cells. The current study attempted to inves...

journal_title：FEBS letters

authors： Burkhardt BR,Lyle R,Qian K,Arnold AS,Cheng H,Atkinson MA,Zhang YC

更新日期：2006-01-23 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:Hydrogen/deuterium (H/D) exchange measurements in low and moderate concentrations of GuHCI were conducted on the side chain H(N) atoms of the seven tryptophans of pseudo wild-type human carbonic anhydrase II. Tryptophans 5, 16 and 245, situated in or close to the N-terminal domain were found to have little protection ...

journal_title：FEBS letters

authors： Jonasson P,Kjellsson A,Sethson I,Jonsson BH

更新日期：1999-02-26 00:00:00

abstract：:Maintaining genomic integrity is vital for cell survival and homeostasis. Mutations in critical genes in germ-line and somatic cells are often implicated with the onset or progression of diseases. DNA repair enzymes thus take important roles as guardians of the genome in the cell. Besides the known function to repair ...

journal_title：FEBS letters

authors： Bunch H

更新日期：2016-04-01 00:00:00

abstract：:Recent studies have implicated that a GTP-binding protein (G-protein) is involved in the coupling of both CCK-8 and muscarinic cholinergic receptors to phosphoinositidase C (PIC) in the human embryonic pituitary cell line, Flow 9000. Pretreatment of these cells with cholera toxin, but not pertussis toxin, inhibited th...

journal_title：FEBS letters

authors： Lo WW,Hughes J

更新日期：1987-08-17 00:00:00

abstract：:In vitro cultured blood cells for transfusion purposes provide a safe alternative to donor blood, particularly for patients who require recurrent transfusions, and can be used as carriers of therapeutic molecules. In vitro derivation of hematopoietic cell types from human-induced pluripotent stem cells (iPSCs) allows ...

journal_title：FEBS letters

authors： Hansen M,von Lindern M,van den Akker E,Varga E

更新日期：2019-12-01 00:00:00

abstract：:Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...

journal_title：FEBS letters

authors： Shariati SA,De Strooper B

更新日期：2013-06-27 00:00:00

abstract：:In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, ...

journal_title：FEBS letters

authors： Costantini P,Chernyak BV,Petronilli V,Bernardi P

更新日期：1995-04-03 00:00:00

abstract：:FACS analysis together with PIPLC treatment was applied to PI-anchoring antigens such as DAF (decay-accerelating factor, CD55), 1F5 antigen (CD59), CD14 and CD16 on the cell surfaces of blood cells from a normal adult and a male patient with paroxysmal nocturnal hemoglubinuria (PNH). Through the extensive analysis, th...

journal_title：FEBS letters

authors： Taguchi R,Funahashi Y,Ikezawa H,Nakashima I

更新日期：1990-02-12 00:00:00

abstract：:Okadaic acid, a specific inhibitor of protein phosphatase 1 in Paramecium causes sustained backward swimming in response to depolarising stimuli (S. Klumpp et al. (1990) EMBO J. 9, 685). Here, we employ okadaic acid, tautomycin, microcystin LR and inhibitor 1 as phosphatase inhibitors to identify a 42 kDa protein in t...

journal_title：FEBS letters

authors： Klumpp S,Schultz JE

更新日期：1991-08-19 00:00:00

abstract：:NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...

journal_title：FEBS letters

authors： Bokui N,Otani T,Igarashi K,Kaku J,Oda M,Nagaoka T,Seno M,Tatematsu K,Okajima T,Matsuzaki T,Ting K,Tanizawa K,Kuroda S

更新日期：2008-01-23 00:00:00

abstract：:In humans, many responses to hypoxia including angiogenesis and erythropoiesis are mediated by the alpha/beta-heterodimeric transcription factor hypoxia inducible factor (HIF). The stability and/or activity of human HIF-1alpha are modulated by post-translational modifications including prolyl and asparaginyl hydroxyla...

journal_title：FEBS letters

authors： Murray-Rust TA,Oldham NJ,Hewitson KS,Schofield CJ

更新日期：2006-04-03 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:Although insulin secretion is usually regarded as a Ca2+-dependent mechanism, recent studies have suggested the existence of a Ca2+-independent pathway of regulation by glucose. Here, mouse islets were used to compare the contribution of Ca2+-dependent and -independent pathways. Glucose increased insulin release in a ...

journal_title：FEBS letters

authors： Sato Y,Nenquin M,Henquin JC

更新日期：1998-01-09 00:00:00

abstract：:Relative amounts of mRNA for cathepsin B were measured in normal murine liver and three murine tumors, an invasive liver tumor (hepatoma, Hepa cl 9) and two melanoma variants (B16-F1 and B16 amelanotic melanoma, B16a). Using a human cDNA to the cathepsin B coding region as a hybridization probe, we detected two specie...

journal_title：FEBS letters

authors： Moin K,Rozhin J,McKernan TB,Sanders VJ,Fong D,Honn KV,Sloane BF

更新日期：1989-02-13 00:00:00

abstract：:Trypsin immobilized on collagen membranes has been used to digest accessible ribosomal proteins of rat liver 40 S subunits. Six proteins (S2, S6, S10, S14, S15 and S25) have been found to be highly exposed on the surface of 40 S particles. They appear to be in close physical contact and localized in the same region of...

journal_title：FEBS letters

authors： Marion MJ,Marion C

更新日期：1988-05-23 00:00:00

abstract：:We have engineered a genomic HDV ribozyme to construct several trans-acting ribozymes for use in trans to cleave target RNAs. Among the 10 different combinations attempted, only HDV88-Trans had cleavage activity on the 13-nucleotide substrate, R13, in vitro. To improve the cleavage efficiency, at least in vitro, of th...

journal_title：FEBS letters

authors： Kawakami J,Yuda K,Suh YA,Kumar PK,Nishikawa F,Maeda H,Taira K,Ohtsuka E,Nishikawa S

更新日期：1996-09-30 00:00:00

abstract：:We have previously identified the binding region of a new Ca2+ antagonist semotiadil in the skeletal muscle Ca2+ channel. To the same semotiadil derivatives, the cardiac counterpart showed distinct and different binding characteristics: semotiadil and its photoaffinity analog D51-4700 inhibited [3H]PN200-110 binding t...

journal_title：FEBS letters

authors： Ii N,Kuniyasu A,Kawahara K,Shibano T,Schwartz A,Nakayama H

更新日期：1998-12-11 00:00:00

abstract：:The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...

journal_title：FEBS letters

authors： Berven LA,Barritt GJ

更新日期：1994-06-13 00:00:00