Changes in the expression of guanine nucleotide-binding proteins during differentiation of 3T3-F442A cells in a hormonally defined medium.

abstract：:Lung surfactant secretion is a highly regulated process. Our previous studies have shown that VAMP-2 is essential for surfactant secretion. In the present study we investigated the role of miR-206 in surfactant secretion through VAMP-2. VAMP-2 was confirmed to be a target of miR-206 by 3'-untranslational region (3'-UT...

journal_title：FEBS letters

authors： Zhang H,Guo Y,Mishra A,Gou D,Chintagari NR,Liu L

更新日期：2015-01-02 00:00:00

abstract：:Human legumain was characterized following overexpression in a murine cell line as the C-terminal Ig-fusion protein. Upon acid treatment, the prolegumain autoproteolyzed distal to two aspartic acid residues to yield a highly active form. The ability of mature legumain to cleave after aspartic acid residues was confirm...

journal_title：FEBS letters

authors： Halfon S,Patel S,Vega F,Zurawski S,Zurawski G

更新日期：1998-10-30 00:00:00

abstract：:Stimuli which enhance secretion from parathyroid cells such as low extracellular Ca2+ or Mg2+ are associated with a decrease in the cytosolic Ca2+ concentration as measured by quin2. Current evidence suggests that increased production of inositol 1,4,5-triphosphate (IP3) releases Ca2+ from cellular stores thus increas...

journal_title：FEBS letters

authors： Epstein PA,Prentki M,Attie MF

更新日期：1985-08-19 00:00:00

abstract：:The reduction of methyl-coenzyme M (CH3SCoM) to methane in methanogenic bacteria is dependent on component B (N-7-mercaptoheptanoyl-O-phospho-L-threonine, HSHTP). We report here that S-methyl-component B (N-7-(methylthio)heptanoyl-O-phospho-L-threonine, CH3SHTP) can substitute for neither CH3SCoM nor HSHTP in the meth...

journal_title：FEBS letters

authors： Ellermann J,Kobelt A,Pfaltz A,Thauer RK

更新日期：1987-08-17 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:Complexes of intact nuclear DNA with proteins undissociable by 2.0 M NaCl and nonionic detergents were analyzed by agarose gel electrophoresis following physical or enzymatic fragmentation. Sulfhydryl molecules converted these DNAs (but not the bacteriophage lambda DNA) into smaller-Mr forms. Following limited restric...

journal_title：FEBS letters

authors： Taş S,De Larco J,Altiok E

更新日期：1985-10-21 00:00:00

abstract：:Tyrosine phosphorylation of Cbl and its association with signal-transducing molecules in response to macrophage colony-stimulating factor (M-CSF) were analyzed by using cell lines which express the wild-type and a mutant M-CSF receptor, Fms. We found that in a clone, F723 TF-1 cells expressing mutant Fms in which tyro...

journal_title：FEBS letters

authors： Ota J,Sato K,Kimura F,Wakimoto N,Nakamura Y,Nagata N,Suzu S,Yamada M,Shimamura S,Motoyoshi K

更新日期：2000-01-21 00:00:00

abstract：:The urokinase receptor (uPAR) on the cell surface plays an important role in extracellular proteolysis, cell migration and adhesion. Soluble uPAR (suPAR) has been recently discovered in plasma, but its origin is unclear. Our results now demonstrate that both unstimulated blood mononuclear and endothelial cells can rel...

journal_title：FEBS letters

authors： Mustjoki S,Sidenius N,Vaheri A

更新日期：2000-12-15 00:00:00

abstract：:The flash-induced voltage response of halorhodopsin at high NaCl concentration comprises two main kinetic components. The first component with tau approximately 1 micros does not exceed 4% of the overall response amplitude and is probably associated with the formation of the L (hR520) intermediate. The second main com...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kalaidzidis YL,Kaulen AD

更新日期：1998-05-01 00:00:00

abstract：:As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-...

journal_title：FEBS letters

authors： Syomin BV,Kandror KV,Semakin AB,Tsuprun VL,Stepanov AS

更新日期：1993-06-01 00:00:00

abstract：:Using the yeast two hybrid system and overlay assays we identified a putative rholrac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C6H2 zinc finger, a PH domain, and a long coile...

journal_title：FEBS letters

authors： Madaule P,Furuyashiki T,Reid T,Ishizaki T,Watanabe G,Morii N,Narumiya S

更新日期：1995-12-18 00:00:00

abstract：:Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were c...

journal_title：FEBS letters

authors： Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet A

更新日期：1998-11-27 00:00:00

abstract：:Beta-thymosins sequester G-actin and preserve a pool of monomers of actin which constitute an important prerequisite for cellular function of the microfilament system. To study the influence of paraquat binding to G-actin on the interaction of G-actin with thymosin beta4 we determined the apparent dissociation constan...

journal_title：FEBS letters

authors： Huff T,Cappelletti G,Hannappel E

更新日期：1998-04-03 00:00:00

abstract：:A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...

journal_title：FEBS letters

authors： Selden C,Jones M,Wade D,Hodgson H

更新日期：1990-09-17 00:00:00

abstract：:Activator protein 1 (AP1) is a complex of Fos and Jun, and it regulates the transcription of genes possessing the AP1-binding sequence. The purpose of this study was to detect living cells that express AP1 after stimulation with a tumor promoter. The Fos and Jun components of AP1 were induced rapidly and transiently i...

journal_title：FEBS letters

authors： Shimokawa N,Miura M

更新日期：1996-06-10 00:00:00

abstract：:Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

journal_title：FEBS letters

authors： Day NJ,Ellenbecker M,Voronina E

更新日期：2018-11-01 00:00:00

abstract：:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

journal_title：FEBS letters

authors： Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

更新日期：2016-09-01 00:00:00

abstract：:The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one ...

journal_title：FEBS letters

authors： Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura T

更新日期：1998-06-12 00:00:00

abstract：:The accumulation of amyloid-beta (Aβ) and tau aggregates is a pathological hallmark of Alzheimer's disease. Both polypeptides form fibrillar deposits, but several lines of evidence indicate that Aβ and tau form toxic oligomeric aggregation intermediates. Depleting such structures could thus be a powerful therapeutic s...

journal_title：FEBS letters

authors： Wobst HJ,Sharma A,Diamond MI,Wanker EE,Bieschke J

更新日期：2015-01-02 00:00:00

abstract：:The stimulatory effects of several DNA-binding basic proteins (histone and protamine) and HIV-1 Rev with arginine (Arg)-rich clusters on the activity of casein kinase II (CK-II) were investigated in vitro. It was found that recombinant Rev (rRev) and the synthetic oligo-fragments corresponding to the amino acid sequen...

journal_title：FEBS letters

authors： Ohtsuki K,Maekawa T,Harada S,Karino A,Morikawa Y,Ito M

更新日期：1998-05-29 00:00:00

abstract：:Five mouse and human leptin receptors (Ob-R) have recently been identified, a long isoform (Ob-Rb), preferentially expressed in hypothalamus, and 4 short isoforms, Ob-Ra, Ob-Rc, Ob-Rd, and Ob-Re. We have identified a new short isoform in the rat, r-OB-Rf, with 6 C-terminal amino acids and a 3' untranslated region with...

journal_title：FEBS letters

authors： Wang MY,Zhou YT,Newgard CB,Unger RH

更新日期：1996-08-26 00:00:00

abstract：:The green fluorescent protein YFP-H148Q is sensitive to halides by a mechanism involving halide binding and a shift in pK(a). However, a limitation of YFP-H148Q is its low halide sensitivity, with K(d)>100 mM for Cl(-). Indicators with improved sensitivities are needed for cell transport studies, particularly in drug ...

journal_title：FEBS letters

authors： Galietta LJ,Haggie PM,Verkman AS

更新日期：2001-06-22 00:00:00

abstract：:Mice deficient in granulocyte-macrophage colony stimulating factor (GM-CSF) develop pulmonary alveolar proteinosis (PAP). We found that bronchoalveolar lavage fluid (BALF) from 11 patients with idiopathic pulmonary alveolar proteinosis (IPAP) suppressed the growth of peripheral blood monocytes and TF-1 cells, a cell l...

journal_title：FEBS letters

authors： Tanaka N,Watanabe J,Kitamura T,Yamada Y,Kanegasaki S,Nakata K

更新日期：1999-01-15 00:00:00

abstract：:Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumor...

journal_title：FEBS letters

authors： Lasmoles F,Minvielle S,Cohen R,Guliana JM,Delehaye MC,Segond N,Calmettes C,Milhaud G,Moukhtar MS

更新日期：1990-12-17 00:00:00

abstract：:T cells express two different types of voltage-independent Ca(2+)-activated K(+) channels with small (SK) and intermediate (IK) conductance that serve important roles in the activation of T lymphocytes. In contrast to the IK channels from T lymphocytes which are upregulated upon mitogen stimulation, SK channels of Jur...

journal_title：FEBS letters

authors： Jäger H,Adelman JP,Grissmer S

更新日期：2000-03-10 00:00:00

abstract：:Focal adhesion (FA) proteins, kindlin-2 and integrin-linked kinase (ILK), regulate cell adhesion and migration. ILK interacts with and promotes kindlin-2 targeting to FAs. Leu353 and Leu357 in kindlin-2 have been reported to be important for the interaction between kindlin-2 and ILK. However, the binding interface bet...

journal_title：FEBS letters

authors： Guan SY,Chng CP,Ong LT,Tan HF,Alex Law SK,Tan SM

更新日期：2018-01-01 00:00:00

abstract：:The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...

journal_title：FEBS letters

authors： Stammers DK,Tisdale M,Court S,Parmar V,Bradley C,Ross CK

更新日期：1991-06-03 00:00:00

abstract：:The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

journal_title：FEBS letters

authors： Wilmen A,Göke B,Göke R

更新日期：1996-11-25 00:00:00

abstract：:The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...

journal_title：FEBS letters

authors： Hobohm U,Sander C

更新日期：1993-11-01 00:00:00

abstract：:In brain tissue a spectrin-like calmodulin-binding protein calspectin, or fodrin, is concentrated in a synaptosome fraction, where most of the calspectin is associated with the synaptic membranes. This endogenous calspectin was phosphorylated by protein kinase system(s) associated with the membranes. Here, we report t...

journal_title：FEBS letters

authors： Sobue K,Kanda K,Kakiuchi S

更新日期：1982-12-13 00:00:00