Abstract:
:The molecular species compositions of monoacylglycerols obtained from various rat tissues were examined by reverse-phase high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS) analyses. We confirmed that 2-arachidonoylglycerol, an endogenous cannabinoid receptor agonist, is one of the most abundant molecular species of monoacylglycerols in the brain. Substantial amounts of 2-arachidonoylglycerol were also found in the liver, spleen, lung and kidney, but the levels were considerably lower than that in the brain. We found that a small amount of 2-arachidonoylglycerol was generated in a brain homogenate during incubation in the absence of Ca2+. Importantly, the generation of 2-arachidonoylglycerol was markedly augmented in the presence of Ca2+, suggesting that Ca2+ plays a key role in regulation of the generation of 2-arachidonoylglycerol in this tissue.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kondo S,Kondo H,Nakane S,Kodaka T,Tokumura A,Waku K,Sugiura Tdoi
10.1016/s0014-5793(98)00581-xsubject
Has Abstractpub_date
1998-06-12 00:00:00pages
152-6issue
2eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00581-Xjournal_volume
429pub_type
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