Abstract:
:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HSdoi
10.1002/1873-3468.12332subject
Has Abstractpub_date
2016-09-01 00:00:00pages
2997-3004issue
17eissn
0014-5793issn
1873-3468journal_volume
590pub_type
信件相关文献
FEBS LETTERS文献大全abstract::Absence through mutation of two proteins involved in periplasmic disulphide bond formation, DsbA and DsbB, results in failure of anaerobically grown Escherichia coli to synthesise the holo forms of either its endogenous c-type cytochrome nitrite reductase or exogenous cytochrome c550 from Paracoccus denitrificans. The...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01256-2
更新日期:1996-12-02 00:00:00
abstract::Recently, reports have appeared which show structural variations in B-DNA and indicate deviations from a uniform helical structure. We report for the first time that these indications are also present in the B-form fibre diffraction patterns for the lithium salt of natural DNA. We have used an improved method of contr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80922-9
更新日期:1984-10-15 00:00:00
abstract::Alternative splicing of the Alzheimer's amyloid beta protein precursor (ABPP) message leads to the production of several variants of this precursor polypeptide. Two of these variants contain a domain that is highly homologous to members of the Kunitz class of protease inhibitors. In order to initiate a study of the ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80926-a
更新日期:1990-07-16 00:00:00
abstract::Several components of translation, e.g. eIF4E and PKR, are implicated in cancer. The e-subunit (p48) of mammalian initiation factor 3 is encoded by the Int6 gene, a common site for integration of the mouse mammary tumor virus genome, leading to the production of a truncated eukaryotic initiation factor-3e (eIF3e). Sta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02307-4
更新日期:2002-03-06 00:00:00
abstract::Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY (transverse relaxation-optimized spectroscopy) has been developed for studies of struc...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(01)02742-9
更新日期:2001-08-31 00:00:00
abstract::The role of necrotic and apoptotic pathways in cytolysis mediated by LAK cells was studied. The contribution of necrotic and apoptotic processes to cytolysis depends both on the LAK cells' phenotype and the type of target cells. CD16+/CD8+/CD3- LAK cells induced necrosis of K562 and L929 target cells. The cell death i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80744-f
更新日期:1993-12-06 00:00:00
abstract::In blood-borne metastasis, intravasated metastatic tumor cells are thought to localize at the target site via a series of processes involving platelet aggregation, adhesion to endothelium, and invasion through the basal membrane. In the present study, we examined how platelet aggregation contributes to the trafficking...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00441-4
更新日期:1998-05-08 00:00:00
abstract::Human ovarian clear cell carcinoma cell line (transferrin (Tf)-non-producer), HAC 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. They synthesized and secreted constantly the 80 kDa protein immun...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81225-d
更新日期:1990-09-17 00:00:00
abstract::Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable S...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80157-x
更新日期:1991-02-25 00:00:00
abstract::Thioredoxin-interacting protein (Txnip) knockout (TKO) mice exhibit impaired response to fasting. Herein, we showed that activation of adenine monophosphate-activated protein kinase and cellular AMP levels were diminished in the heart and soleus muscle but not in gastrocnemius muscle of fasting TKO mice. Similarly, gl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.03.042
更新日期:2011-04-20 00:00:00
abstract::Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80033-f
更新日期:1990-01-01 00:00:00
abstract::A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81155-8
更新日期:1985-03-11 00:00:00
abstract::The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80557-j
更新日期:1991-05-20 00:00:00
abstract::Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80807-f
更新日期:1991-07-22 00:00:00
abstract::We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.098
更新日期:2010-02-05 00:00:00
abstract::Novel peptides containing the sequence -Pro-Leu-Ama(NHOH)- were synthesized and characterized by spectroscopic techniques. Their inhibitory properties towards the activated form of native human gelatinase B (MMP-9) and the catalytic domain of neutrophil collagenase (cdMMP-8) were determined. The most effective inhibit...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01128-4
更新日期:1998-10-02 00:00:00
abstract::We have characterized the Bacillus subtilis homologs of fructoselysine 6-kinase and fructoselysine-6-phosphate deglycase, two enzymes that specifically metabolize the Amadori compound fructose-epsilon-lysine in Escherichia coli. The B. subtilis enzymes also catalyzed the phosphorylation of fructosamines to fructosamin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.10.049
更新日期:2004-11-19 00:00:00
abstract::In vitro cultured blood cells for transfusion purposes provide a safe alternative to donor blood, particularly for patients who require recurrent transfusions, and can be used as carriers of therapeutic molecules. In vitro derivation of hematopoietic cell types from human-induced pluripotent stem cells (iPSCs) allows ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13599
更新日期:2019-12-01 00:00:00
abstract::LLC-PK1L cells, a kidney-derived cell line grown in defined medium, possess a vasopressin-sensitive adenylate cyclase. Somatostatin was able to inhibit the vasopressin-induced increase in adenylate cyclase activity, without affecting the basal enzyme activity. This inhibition was competitive. No effect of somatostatin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80304-x
更新日期:1984-04-24 00:00:00
abstract::125I-labelled angiotensin II (A II) specifically binds to solubilized receptors extracted from rat isolated glomeruli using CHAPS (3-[3-( cholamidopropyl ) dimethylammonio ]-1-propanesulfonate). The yield of solubilization of the binding sites was 3.3%. Equilibrium was reached after 15-20 min and specific binding repr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81321-6
更新日期:1984-05-21 00:00:00
abstract::Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01039-x
更新日期:1999-08-27 00:00:00
abstract::We have previously shown that unsaturated phosphatidylserines inhibit mitogen-induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81724-e
更新日期:1993-01-18 00:00:00
abstract::Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80295-r
更新日期:1992-01-27 00:00:00
abstract::Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.11.065
更新日期:2007-12-22 00:00:00
abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03081-2
更新日期:2001-11-23 00:00:00
abstract::In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2006.12.029
更新日期:2007-01-23 00:00:00
abstract::The topic synthetic biology appears still as an 'empty basket to be filled'. However, there is already plenty of claims and visions, as well as convincing research strategies about the theme of synthetic biology. First of all, synthetic biology seems to be about the engineering of biology - about bottom-up and top-dow...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.05.033
更新日期:2012-07-16 00:00:00
abstract::The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)80036-8
更新日期:1984-01-23 00:00:00
abstract::Insulin-induced changes in protein kinase C were examined in cytosol and membrane fractions of rat adipocytes enzymatically after Mono Q column chromatography and by immunoblotting. During a 5-20 min period of insulin treatment, cytosolic protein kinase C decrease by approximately 50%, whereas membrane protein kinase ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81565-0
更新日期:1989-11-06 00:00:00
abstract::The ability of the Escherichia coli single-stranded DNA-binding protein (SSB) to recognize structural features associated with intramolecular triplex formation in oligopurine.oligopyrimidine (pur.pyr) inserts in recombinant plasmids was evaluated. The SSB protein binds to supercoiled plasmids and causes a site-prefere...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80666-i
更新日期:1993-11-01 00:00:00