Structural identification of the lipopolysaccharide-binding capability of a cupin-family protein from Helicobacter pylori.

Abstract:

:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry and NMR titration demonstrated that HP0902 is able to bind bacterial endotoxin lipopolysaccharides (LPS) through its surface-exposed loops, where metal-binding sites are usually found in other metallo-cupins. This report constitutes the first identification of an LPS-interacting protein, both in the cupin family and in H. pylori. Furthermore, identification of the ability of HP0902 to bind LPS uncovers a putative role for this protein in H. pylori pathogenicity.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

doi

10.1002/1873-3468.12332

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

2997-3004

issue

17

eissn

0014-5793

issn

1873-3468

journal_volume

590

pub_type

信件
  • Mutants of Escherichia coli lacking disulphide oxidoreductases DsbA and DsbB cannot synthesise an exogenous monohaem c-type cytochrome except in the presence of disulphide compounds.

    abstract::Absence through mutation of two proteins involved in periplasmic disulphide bond formation, DsbA and DsbB, results in failure of anaerobically grown Escherichia coli to synthesise the holo forms of either its endogenous c-type cytochrome nitrite reductase or exogenous cytochrome c550 from Paracoccus denitrificans. The...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(96)01256-2

    authors: Sambongi Y,Ferguson SJ

    更新日期:1996-12-02 00:00:00

  • Fibre diffraction of lithium DNA shows structural variability and deviation from a regular helical structure for the B-form.

    abstract::Recently, reports have appeared which show structural variations in B-DNA and indicate deviations from a uniform helical structure. We report for the first time that these indications are also present in the B-form fibre diffraction patterns for the lithium salt of natural DNA. We have used an improved method of contr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80922-9

    authors: Dutta S,Parrack PK,Sasisekharan V

    更新日期:1984-10-15 00:00:00

  • Does the Kunitz domain from the Alzheimer's amyloid beta protein precursor inhibit a kallikrein responsible for post-translational processing of nerve growth factor precursor?

    abstract::Alternative splicing of the Alzheimer's amyloid beta protein precursor (ABPP) message leads to the production of several variants of this precursor polypeptide. Two of these variants contain a domain that is highly homologous to members of the Kunitz class of protease inhibitors. In order to initiate a study of the ph...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)80926-a

    authors: Castro M,Marks CB,Nilsson B,Anderson S

    更新日期:1990-07-16 00:00:00

  • Malignant transformation by the eukaryotic translation initiation factor 3 subunit p48 (eIF3e).

    abstract::Several components of translation, e.g. eIF4E and PKR, are implicated in cancer. The e-subunit (p48) of mammalian initiation factor 3 is encoded by the Int6 gene, a common site for integration of the mouse mammary tumor virus genome, leading to the production of a truncated eukaryotic initiation factor-3e (eIF3e). Sta...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)02307-4

    authors: Mayeur GL,Hershey JW

    更新日期:2002-03-06 00:00:00

  • Solution NMR studies of the integral membrane proteins OmpX and OmpA from Escherichia coli.

    abstract::Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY (transverse relaxation-optimized spectroscopy) has been developed for studies of struc...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/s0014-5793(01)02742-9

    authors: Fernández C,Hilty C,Bonjour S,Adeishvili K,Pervushin K,Wüthrich K

    更新日期:2001-08-31 00:00:00

  • The role of apoptotic and necrotic processes in cytolysis mediated by LAK cells with different phenotypes.

    abstract::The role of necrotic and apoptotic pathways in cytolysis mediated by LAK cells was studied. The contribution of necrotic and apoptotic processes to cytolysis depends both on the LAK cells' phenotype and the type of target cells. CD16+/CD8+/CD3- LAK cells induced necrosis of K562 and L929 target cells. The cell death i...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80744-f

    authors: Sashchenko LP,Kabanova OD,Blishchenko LYu,Lukjianova TI,Satpaev DK,Chertov OYu,Gnuchev NV

    更新日期:1993-12-06 00:00:00

  • How platelet aggregation affects B16BL6 melanoma cell trafficking.

    abstract::In blood-borne metastasis, intravasated metastatic tumor cells are thought to localize at the target site via a series of processes involving platelet aggregation, adhesion to endothelium, and invasion through the basal membrane. In the present study, we examined how platelet aggregation contributes to the trafficking...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)00441-4

    authors: Koike C,Watanabe M,Isoai A,Kumagai H,Tsukada H,Irimura T,Okada S,Oku N

    更新日期:1998-05-08 00:00:00

  • Clear cell carcinoma of the human ovary synthesizes and secretes a transferrin with microheterogeneity of lectin affinity.

    abstract::Human ovarian clear cell carcinoma cell line (transferrin (Tf)-non-producer), HAC 2, cells were adapted to grow in chemically defined synthetic medium when the cells were cultured with medium containing 10 micrograms/ml of insulin at least for 6 months. They synthesized and secreted constantly the 80 kDa protein immun...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)81225-d

    authors: Ohkawa K,Takada K,Takizawa N,Hatano T,Tsukada Y,Matsuda M

    更新日期:1990-09-17 00:00:00

  • Oxidation/reduction explains heterogeneity of pancreatic somatostatin.

    abstract::Somatostatin 14 (SS 14) has been isolated from pancreatic extracts, but open gel filtration immunoreactive SS often elutes in two peaks. We isolated both peaks, but upon sequence analysis only authentic SS 14 could be identified. By further gel filtration experiments it turned out that both synthetic and extractable S...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80157-x

    authors: Bersani M,Johnsen AH,Holst JJ

    更新日期:1991-02-25 00:00:00

  • Diminished AMPK signaling response to fasting in thioredoxin-interacting protein knockout mice.

    abstract::Thioredoxin-interacting protein (Txnip) knockout (TKO) mice exhibit impaired response to fasting. Herein, we showed that activation of adenine monophosphate-activated protein kinase and cellular AMP levels were diminished in the heart and soleus muscle but not in gastrocnemius muscle of fasting TKO mice. Similarly, gl...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2011.03.042

    authors: Andres AM,Ratliff EP,Sachithanantham S,Hui ST

    更新日期:2011-04-20 00:00:00

  • Fatty acid acylation of membrane skeletal proteins in human erythrocytes.

    abstract::Fatty acid acylation of membrane proteins was studied on human erythrocytes by measuring incorporation of [3H]palmitate at different specific radioactivities. A 55 kDa polypeptide within the band 4.5 region was the main acceptor protein for acylation by fatty acids (palmitate, stearate, oleate), while other polypeptid...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)80033-f

    authors: Maretzki D,Mariani M,Lutz HU

    更新日期:1990-01-01 00:00:00

  • Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles. Interaction with cytoskeletal protein - vinculin.

    abstract::A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinc...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)81155-8

    authors: Koteliansky VE,Gneushev GN,Belkin AM

    更新日期:1985-03-11 00:00:00

  • Alternative splicing generates two isoforms of the alpha 2 subunit of the inhibitory glycine receptor.

    abstract::The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha 2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80557-j

    authors: Kuhse J,Kuryatov A,Maulet Y,Malosio ML,Schmieden V,Betz H

    更新日期:1991-05-20 00:00:00

  • Does ferredoxin I (Azotobacter) represent a novel class of DNA-binding proteins that regulate gene expression in response to cellular iron(II)?

    abstract::Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80807-f

    authors: Thomson AJ

    更新日期:1991-07-22 00:00:00

  • Activation of oxidative stress response by hydroxyl substituted chalcones and cyclic chalcone analogues in mitochondria.

    abstract::We investigated the effect of hydroxyl substituted chalcone (1a) and some chalcone analogues (1b-d) on isolated rat liver mitochondria to gain new insights into the cytotoxic mechanism of these compounds. We observed an inhibitory effect on phosphorylation and the partial uncoupling of compounds 1a and 1d. Increased r...

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    pub_type: 杂志文章

    doi:10.1016/j.febslet.2009.11.098

    authors: Guzy J,Vasková-Kubálková J,Rozmer Z,Fodor K,Mareková M,Poskrobová M,Perjési P

    更新日期:2010-02-05 00:00:00

  • Hydroxamate derivatives of substrate-analogous peptides containing aminomalonic acid are potent inhibitors of matrix metalloproteinases.

    abstract::Novel peptides containing the sequence -Pro-Leu-Ama(NHOH)- were synthesized and characterized by spectroscopic techniques. Their inhibitory properties towards the activated form of native human gelatinase B (MMP-9) and the catalytic domain of neutrophil collagenase (cdMMP-8) were determined. The most effective inhibit...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01128-4

    authors: Krumme D,Wenzel H,Tschesche H

    更新日期:1998-10-02 00:00:00

  • Identification of enzymes acting on alpha-glycated amino acids in Bacillus subtilis.

    abstract::We have characterized the Bacillus subtilis homologs of fructoselysine 6-kinase and fructoselysine-6-phosphate deglycase, two enzymes that specifically metabolize the Amadori compound fructose-epsilon-lysine in Escherichia coli. The B. subtilis enzymes also catalyzed the phosphorylation of fructosamines to fructosamin...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.10.049

    authors: Wiame E,Duquenne A,Delpierre G,Van Schaftingen E

    更新日期:2004-11-19 00:00:00

  • Human-induced pluripotent stem cell-derived blood products: state of the art and future directions.

    abstract::In vitro cultured blood cells for transfusion purposes provide a safe alternative to donor blood, particularly for patients who require recurrent transfusions, and can be used as carriers of therapeutic molecules. In vitro derivation of hematopoietic cell types from human-induced pluripotent stem cells (iPSCs) allows ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1002/1873-3468.13599

    authors: Hansen M,von Lindern M,van den Akker E,Varga E

    更新日期:2019-12-01 00:00:00

  • Inhibition by somatostatin of the vasopressin-stimulated adenylate cyclase in a kidney-derived line of cells grown in defined medium.

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    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80304-x

    authors: Roy C

    更新日期:1984-04-24 00:00:00

  • Solubilization of angiotensin II receptors in rat glomeruli.

    abstract::125I-labelled angiotensin II (A II) specifically binds to solubilized receptors extracted from rat isolated glomeruli using CHAPS (3-[3-( cholamidopropyl ) dimethylammonio ]-1-propanesulfonate). The yield of solubilization of the binding sites was 3.3%. Equilibrium was reached after 15-20 min and specific binding repr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)81321-6

    authors: Chansel D,Ardaillou N,Ardaillou R

    更新日期:1984-05-21 00:00:00

  • Overexpression of poly(A)-binding protein down-regulates the translation or the abundance of its own mRNA.

    abstract::Poly(A)-binding protein (PABP) mRNA is subject to autoregulation through a 61 nucleotides long A-rich sequence in its 5' untranslated region (UTR). Here, we show that this mode of regulation is exerted in a cell type-specific manner. Thus, overexpression of PABP in mouse NIH 3T3 fibroblasts represses the translation o...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)01039-x

    authors: Hornstein E,Harel H,Levy G,Meyuhas O

    更新日期:1999-08-27 00:00:00

  • Role of a serum phospholipase A1 in the phosphatidylserine-induced T cell inhibition.

    abstract::We have previously shown that unsaturated phosphatidylserines inhibit mitogen-induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81724-e

    authors: Bellini F,Bruni A

    更新日期:1993-01-18 00:00:00

  • Stable expression of high affinity NK1 (substance P) and NK2 (neurokinin A) receptors but low affinity NK3 (neurokinin B) receptors in transfected CHO cells.

    abstract::Stable CHO cell clones which selectively express all three rat tachykinin receptors were established by transfection. The binding of radiolabled substance P and neurokinin A (substance K) to CHO clones expressing the NK1 and NK2 receptors, respectively, were saturatable and of high affinity (Kd = 0.17 nM (NK1); 3.4 nM...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(92)80295-r

    authors: Gether U,Marray T,Schwartz TW,Johansen TE

    更新日期:1992-01-27 00:00:00

  • Direct neurite-osteoblastic cell communication, as demonstrated by use of an in vitro co-culture system.

    abstract::Using an in vitro co-culture approach comprising cultured murine superior cervical ganglia and MC3T3-E1 osteoblast-like cells, we found that the addition of scorpion venom (SV) elicited neurite activation via intracellular Ca2+ mobilization and, after a lag period, osteoblastic Ca2+ mobilization. SV did not have any d...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.11.065

    authors: Obata K,Furuno T,Nakanishi M,Togari A

    更新日期:2007-12-22 00:00:00

  • Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

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    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03081-2

    authors: Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

    更新日期:2001-11-23 00:00:00

  • Beyond the epithelium: cadherin function in fibrous connective tissues.

    abstract::In fibrous connective tissues, fibroblasts are organized into syncytia, cellular networks that enable matrix remodeling and that are interconnected by intercellular adherens junctions (AJs). The AJs of fibroblasts are mediated by N-cadherin, a broadly expressed classical cadherin that is critically involved in develop...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2006.12.029

    authors: El Sayegh TY,Kapus A,McCulloch CA

    更新日期:2007-01-23 00:00:00

  • Synthetic biology, inspired by synthetic chemistry.

    abstract::The topic synthetic biology appears still as an 'empty basket to be filled'. However, there is already plenty of claims and visions, as well as convincing research strategies about the theme of synthetic biology. First of all, synthetic biology seems to be about the engineering of biology - about bottom-up and top-dow...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2012.05.033

    authors: Malinova V,Nallani M,Meier WP,Sinner EK

    更新日期:2012-07-16 00:00:00

  • Amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria and its homology with the delta-subunit of the F1-ATPase of Escherichia coli.

    abstract::The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(84)80036-8

    authors: Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

    更新日期:1984-01-23 00:00:00

  • Insulin stimulates the translocation of protein kinase C in rat adipocytes.

    abstract::Insulin-induced changes in protein kinase C were examined in cytosol and membrane fractions of rat adipocytes enzymatically after Mono Q column chromatography and by immunoblotting. During a 5-20 min period of insulin treatment, cytosolic protein kinase C decrease by approximately 50%, whereas membrane protein kinase ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)81565-0

    authors: Ishizuka T,Cooper DR,Farese RV

    更新日期:1989-11-06 00:00:00

  • Escherichia coli single-stranded DNA-binding protein alters the structure of intramolecular triplexes in plasmids.

    abstract::The ability of the Escherichia coli single-stranded DNA-binding protein (SSB) to recognize structural features associated with intramolecular triplex formation in oligopurine.oligopyrimidine (pur.pyr) inserts in recombinant plasmids was evaluated. The SSB protein binds to supercoiled plasmids and causes a site-prefere...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80666-i

    authors: Klysik J,Shimizu M

    更新日期:1993-11-01 00:00:00