Abstract:
:Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron-sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were chemically reconstituted with FeCl3 and Na2[34S] to give labelled (Fe34S) enzymes. These enzymes were functional and when assayed in vitro produced labelled biotin containing about 65% of 34S. These data strongly support the hypothesis that the sulphur of biotin is derived from the (FeS) centre of the enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bui BT,Florentin D,Fournier F,Ploux O,Méjean A,Marquet Adoi
10.1016/s0014-5793(98)01464-1subject
Has Abstractpub_date
1998-11-27 00:00:00pages
226-30issue
1-2eissn
0014-5793issn
1873-3468pii
S0014579398014641journal_volume
440pub_type
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