Abstract:
:The cyoE gene of the Escherichia coli bo-type quinol oxidase operon (cyoABCDE) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa3-type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic complementation analysis using the chimeric operon cyoABCD-caaE and heme O synthase assay using the CaaE-overproduced E. coli membranes. We found that the caaE gene encodes a thermotolerant heme O synthase which provides an intermediate for heme A biosynthesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Saiki K,Mogi T,Ishizuka M,Anraku Ydoi
10.1016/0014-5793(94)00889-2subject
Has Abstractpub_date
1994-09-12 00:00:00pages
385-8issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00889-2journal_volume
351pub_type
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