YY1 is involved in the regulation of the bi-directional promoter of the Surf-1 and Surf-2 genes.

Abstract:

:The Surfeit locus is an unusual cluster of at least 6 housekeeping genes whose organisation is conserved between birds and mammals. We have previously shown that the divergently transcribed Surf-1 and Surf-2 genes are separated by a bi-directional, TATA-less promoter. In mouse, the Surf-1/Surf-2 promoter contains three important factor binding sites: Su1, Su2, and Su3. These sites are conserved between the mouse and human Surf-1/Surf-2 promoters, bind nuclear factors in vitro, and are required for accurate and efficient expression of Surf-1 and Surf-2 in vivo. Using gel retardation assays, methylation interference experiments, and specific antibodies we demonstrate that the Su1 binding factor is the initiator protein YY1. Over-expression of YY1 results in a major stimulation of transcription in the Surf-1 direction and a minor stimulation of transcription in the Surf-2 direction.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Gaston K,Fried M

doi

10.1016/0014-5793(94)00567-2

subject

Has Abstract

pub_date

1994-06-27 00:00:00

pages

289-94

issue

2-3

eissn

0014-5793

issn

1873-3468

pii

0014-5793(94)00567-2

journal_volume

347

pub_type

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