Abstract:
:We employed cDNA representational difference analysis to identify new genes that are upregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in a human hepatoblastoma cell line HepG2. We isolated several TCDD-responsive cDNAs. Sequence analysis revealed that one of them encodes E16/CD98LC/hLAT1, an integral membrane protein involved in multiple cellular functions including cellular transport of L-type amino acids. Northern blot analysis confirmed the TCDD-dependent upregulation of the mRNA. Induction of E16/CD98LC/hLAT1 mRNA by TCDD did not require de novo protein synthesis as revealed by the experiment using cycloheximide. Consistent with the changes at mRNA level, the transport of 3H-leucine into HepG2 cells was significantly increased by TCDD treatment. These findings provide a novel aspect of biological effects of TCDD on human hepatocytes.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sarkar D,Kambe F,Hirata A,Iseki A,Ohmori S,Seo Hdoi
10.1016/s0014-5793(99)01574-4keywords:
subject
Has Abstractpub_date
1999-12-03 00:00:00pages
430-4issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01574-4journal_volume
462pub_type
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