Abstract:
:Highly active adenylylsulfate (APS) reductase was isolated under N(2)/H(2) from sulfate-reducing and sulfide-oxidizing bacteria and archaea. It was a 1:1 alphabeta-heterodimer of molecular mass approximately 95 kDa, and two subunits (alpha approximately 75, beta approximately 20 kDa). The specific activity was 11-14 micromol (min mg)(-1); cofactor analysis revealed 0.96+/-0.05 FAD, 7.5+/-0.1 Fe and 7.9+/-0.25 S(2-). The photochemically reduced enzyme had a multiline EPR spectrum resulting from two interacting [4Fe-4S] centers. The properties of the different APS reductases were remarkably similar, although the enzyme is involved in different metabolic pathways and was isolated from phylogenetically far separated organisms. A structural model is proposed, with FAD bound to the alpha-subunit, and two [4Fe-4S] centers located in close proximity on the beta-subunit.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Fritz G,Büchert T,Huber H,Stetter KO,Kroneck PMdoi
10.1016/s0014-5793(00)01500-3keywords:
subject
Has Abstractpub_date
2000-05-04 00:00:00pages
63-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01500-3journal_volume
473pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Dendritic cells (DCs) are known to modulate immune response by activating effector cells of both the innate and the adaptive immune system. In the present study, we demonstrate that co-culture of DCs with paraformaldehyde-fixed tumor cells augments the secretion of interleukin (IL)-12 by DCs and these activated DCs up...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00026-2
更新日期:2004-02-13 00:00:00
abstract::Non-invasive selection of transgenic mice was performed at the stage of preimplantation embryos. The morulae collected from wild female mated with hemizygous transgenic male expressing Aequorea victoria green fluorescent protein (GFP) under chicken beta-actin promoter could be classified as green or non-green under a ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01162-8
更新日期:1995-11-13 00:00:00
abstract::By co-expression of heme oxygenase and various bilin reductase(s) in a single operon in conjunction with apophytochrome using two compatible plasmids, we developed a system to produce phytochromes with various chromophores in Escherichia coli. Through the selection of different bilin reductases, apophytochromes were a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.051
更新日期:2006-02-20 00:00:00
abstract::Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key glycolytic enzyme regulated by many diverse mechanisms. In this study we present evidence that GAPDH activity is stimulated in the presence of oxyhemoglobin (2.3-fold, P < 0.005). No stimulation was seen by myoglobin, and only slight stimulation (1.2-fold, not ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01181-2
更新日期:1997-10-13 00:00:00
abstract::We have generated a mouse model for hepatic ischemia in which surgical subcutaneous transposition of the spleen allows hepatic ischemia to be applied without affecting other tissues. Using this mouse model we investigated the relationship between the length of ischemic periods in the liver and subsequent liver functio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01517-2
更新日期:1997-12-29 00:00:00
abstract::The oxidation of free methionine to methionine sulfoxide by chemically or enzymatically generated oxygen free radicals is presented. The physiological significance of this process in living cells is suggested. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80443-x
更新日期:1987-11-16 00:00:00
abstract::Local bending propensity and curvature of DNA can be characterized using a vector description of DNA bendability, based on a set of parameters derived from deoxyribonuclease I (DNase I) cleavage experiments. Two characteristics-arithmetic and vector averages of bendability-were successfully used to predict experimenta...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00837-x
更新日期:1996-09-09 00:00:00
abstract::Azotobacter vinelandii (Av) and chroococcum (Ac) ferredoxin I contain [3Fe-4S]1 + 0 and [4Fe-4S]2+1+ clusters, when isolated aerobically, which undergo one-electron redox cycles at potentials of -460 +/- 10 mV (vs SHE) at pH 8.3 and -645 +/- 10 mV, respectively. The X-ray structure of Fd I (Av) reveals that the N-term...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80807-f
更新日期:1991-07-22 00:00:00
abstract::Infantile and juvenile neuronal ceroid lipofuscinosis (NCLs) are progressive neurodegenerative disorders of childhood with distinct ages of clinical onset, but with a similar pathological outcome. Infantile and juvenile NCL are inherited in an autosomal recessive manner due to mutations in the CLN1 and CLN3 genes, res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00162-5
更新日期:2003-03-13 00:00:00
abstract::Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80047-x
更新日期:1993-03-15 00:00:00
abstract::The plant hormone cytokinin plays a major role in regulating plant growth and development. Here we generated cytokinin-reduction Arabidopsis plants by overexpressing a heterologous cytokinin oxidase gene DSCKX1 from Dendrobium orchid. These transgenic plants exhibited reduced biomass, rapid root growth, decreased abil...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01259-6
更新日期:2003-12-04 00:00:00
abstract::Chemokines play a key role in modulating leukocyte functions at sites of inflammation. To assess chondrocyte contribution to the chemotactic environment of inflamed joints the intracellular content of CC and CXC chemokines was investigated. IL-8, GROalpha, MCP-1, RANTES, MIP-1alpha and MIP-1beta expression was evaluat...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00886-8
更新日期:1999-07-23 00:00:00
abstract::Endothelial dysfunction plays a major role in the pathogenesis of atherosclerosis. Pro-inflammatory cytokines such as interleukin-1 beta and tumour necrosis factor alpha activate endothelial cells changing their resting phenotype to become pro-adhesive, pro-thrombotic and pro-atherogenic. Phage display in vivo biopann...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02191-3
更新日期:2001-03-09 00:00:00
abstract::We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00497-4
更新日期:1999-05-07 00:00:00
abstract::AQP3 is a water/glycerol transporter expressed at the basolateral membrane of colonic epithelial cells. Although AQPs are expressed in the gastrointestinal tract, their effect on intestinal barrier has not been clear. Here, we showed that knockdown of AQP3 caused a dramatic, dose-dependent increase in E. coli C25 tran...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.08.045
更新日期:2011-10-03 00:00:00
abstract::Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.10.051
更新日期:2008-12-10 00:00:00
abstract::Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81331-s
更新日期:1993-02-22 00:00:00
abstract::Human preimplantation developmental studies are difficult to accomplish due to associated ethical and moral issues. Preimplantation cells are rare and exist only in transient cell states. From a single cell, it is very challenging to analyse the origination of the heterogeneity and complexity inherent to the human bod...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12716
更新日期:2017-08-01 00:00:00
abstract::In order to get a better insight into the function of amino acid residues located in the second transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, all exon 18 mutations found in cystic fibrosis (CF) patients were characterized at the protein and at the electrophysiological ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01042-4
更新日期:1998-10-16 00:00:00
abstract::The synthesis of gangliosides GM3 and GD3 is carried out by the successive addition of sialic acid residues on lactosylceramide (LacCer) by the Golgi located sialyltransferases Sial-T1 and Sial-T2, respectively. CHO-K1 cells lack Sial-T2 and only express GM3. Here we show that the activity of Sial-T1 was near 2.5-fold...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.05.041
更新日期:2012-07-30 00:00:00
abstract::The fluorescence dye 1-anilinonaphthalene-8-sulfonate (ANS) was used as a probe of non-polar binding sites in 4-aminobutyrate aminotransferase. ANS binds to a single binding site of the dimeric protein with a Kd of 6 microM. Nanosecond emission anisotropy measurements were performed on the ANS-enzyme in an effort to d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80971-5
更新日期:1983-08-22 00:00:00
abstract::Interactions of U2 snRNA with anti-Sm precipitable proteins in RNA-free cytoplasmic complexes were analyzed. U2 snRNA was found to bind specifically with proteins in the 6S complexes but not in the 20S complexes. The binding activity was preserved using U2 snRNA having a mutated Sm binding site. Label-transfer experim...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00095-1
更新日期:1997-03-03 00:00:00
abstract::Tissue transglutaminase is a multifunctional enzyme which has been involved in the regulation of cell growth, differentiation, and apoptosis. Recently, nuclear localization of tTG has been reported indicating the potential of active nuclear transport. In this study we use the yeast two-hybrid assay and co-immunoprecip...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00018-6
更新日期:1999-03-05 00:00:00
abstract::Bacteriophage T5 Bg/II/Hind III DNA fragment (803 basepairs), containing the genes for 2 tRNAs and 2 RNAs with unknown functions, was cloned in the plasmid pBR322. The analysis of DNA sequence indicates that tRNA genes code isoacceptor tRNAsSer (tRNA1Ser and tRNA2Ser) with anticodons UGA and GGA, respectively. The mai...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80690-5
更新日期:1983-07-11 00:00:00
abstract::By applying the 'recognition mask' strategy to 300 mammalian sequences containing NotI sites we demonstrated that 5' ends of genes are highly enriched in NotI sites. A NotI linking clone NL2-252 (D3S1678) containing transferrin receptor (TFRC) gene was used as an initial point for chromosomal jumping. One of the jumpi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01449-x
更新日期:1997-12-15 00:00:00
abstract::Photoaffinity labelling with [alpha-32P]GTP allowed to detect a 54 kDa GTP-binding protein in rat pancreatic plasma membranes and in pancreatic AR 4-2J cell membranes. Like the 42 and 48 kDa Gs alpha subunits and the 41 kDa Gi alpha subunit, this protein was absent from zymogen granule membranes. Contrastingly, a new ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80362-m
更新日期:1990-10-01 00:00:00
abstract::Alternative splicing of the Alzheimer's amyloid beta protein precursor (ABPP) message leads to the production of several variants of this precursor polypeptide. Two of these variants contain a domain that is highly homologous to members of the Kunitz class of protease inhibitors. In order to initiate a study of the ph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80926-a
更新日期:1990-07-16 00:00:00
abstract::The substrate peptide bond cleaved by cathepsins B and L is determined not by the amino acid contributing the carboxyl group to this bond as in the case of serine proteases but rather by the presence of a neighboring amino acid with a large hydrophobic side chain. From a study of the inhibitory potency in a series, Cb...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80600-8
更新日期:1988-02-08 00:00:00
abstract::Hemin stimulates cAMP production in human peripheral blood mononuclear cells (PBMC). The kinetics are similar to that of hormone-induced cAMP generation, namely a rapid effect followed by a desensitization phase. Several experimental findings suggest that prostaglandins do not mediate this effect. First, macrophage de...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80529-p
更新日期:1992-06-01 00:00:00
abstract::Recently the identity of the peptidyl-prolyl cis-trans isomerase (PPIase), which accelerates the cis/trans isomerization of prolyl peptide bonds and cyclophilin, the binding protein for the immunosuppressive drug Cyclosporin A (CsA), was discovered. The PPIase catalysis toward the substrate Suc-Ala-Phe-Pro-Phe-pNA has...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80766-v
更新日期:1991-06-17 00:00:00