In vitro interaction of U2 snRNA with cytoplasmic 6S protein complexes.

abstract：:Macrophages are abundant in the tumor microenvironment. They are highly plastic and able to acquire pro-tumoral phenotypes in response to microenvironmental stimuli. When we treated RAW 264.7 macrophages with inhibitors of various oncogenic pathways, we found that the focal adhesion kinase (FAK) inhibitors PF573228 an...

journal_title：FEBS letters

authors： He X,Chen X,Li B,Ji J,Chen S

更新日期：2017-12-01 00:00:00

abstract：:Histone H1(0) is found in tissues with little or no cellular proliferation and has been shown to accumulate during cellular terminal differentiation. Two subtypes of H1(0), H1 a and H1 b, are present in any tissue where the protein has been detected. We report here the first evidence of an age-dependent change in the ...

journal_title：FEBS letters

authors： Piña B,Suau P

更新日期：1987-01-05 00:00:00

abstract：:Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...

journal_title：FEBS letters

authors： Goueli SA,Hanten JA,Ahmed K

更新日期：1991-05-06 00:00:00

abstract：:Crk and CrkL adaptors play essential neuronal positioning roles downstream of Reelin-induced Dab1 tyrosine phosphorylation. Recently we identified Cin85 to be a CrkL-SH3 binding partner from embryonic murine brain while others found Cin85 binds directly to Dab1. Here using mass spectrometry, biochemical and mutational...

journal_title：FEBS letters

authors： Bior BK,Ballif BA

更新日期：2013-01-04 00:00:00

abstract：:The 23-residue synthetic peptide representing the N-terminus of HIV-1 gp41 is known to induce either leakage or fusion of lipid vesicles depending on the experimental conditions. In this paper we report that a polar amino acid substitution V-->E at position 2, known to block gp41 activity in vivo, makes the peptide un...

journal_title：FEBS letters

authors： Pereira FB,Goñi FM,Nieva JL

更新日期：1995-04-03 00:00:00

abstract：:In this review, we summarize the currently available information on the membrane topology of some key members of the human ABC protein subfamilies, and present the predicted domain arrangements. In the lack of high-resolution structures for eukaryotic ABC transporters this topology is based only on prediction algorith...

journal_title：FEBS letters

authors： Tusnády GE,Sarkadi B,Simon I,Váradi A

更新日期：2006-02-13 00:00:00

abstract：:The effects of intracellular purine nucleotides on tolbutamide-induced block of ATP-dependent K+ channels from mouse pancreatic B-cells were studied using the patch-clamp technique. When applied to the inside of excised patches, tolbutamide alone blocked channel activity half-maximally at 55 microM and the concentrati...

journal_title：FEBS letters

authors： Zünkler BJ,Lins S,Ohno-Shosaku T,Trube G,Panten U

更新日期：1988-11-07 00:00:00

abstract：:A human liver cDNA clone which encodes the UDP-glucose pyrophosphorylase was isolated by complementation of a bacterial galU mutant. The deduced amino acid sequence of the human enzyme comprised 508 amino acids with a calculated molecular mass of 56,950. The human enzyme significantly resembles those of potato tuber a...

journal_title：FEBS letters

authors： Peng HL,Chang HY

更新日期：1993-08-23 00:00:00

abstract：:The modification of homeodomain-interacting protein kinase 2 (HIPK2) by small ubiquitin-like modifier 1 (SUMO-1) plays an important role in its targeting into the promyelocytic leukemia body, as well as in its differential interaction with binding partner, but the desumoylation of HIPK2 by SUMO-specific proteases is l...

journal_title：FEBS letters

authors： Kim YH,Sung KS,Lee SJ,Kim YO,Choi CY,Kim Y

更新日期：2005-11-07 00:00:00

abstract：:A long-term goal of the field of interactome modeling is to understand how global and local properties of complex macromolecular networks impact on observable biological properties, and how changes in such properties can lead to human diseases. The information available at this stage of development of the field provid...

journal_title：FEBS letters

authors： Vidal M

更新日期：2005-03-21 00:00:00

abstract：:Induction of a microsomal oleate delta12 (n-6) desaturase which is mainly responsible for an increase in membrane lipid unsaturation at low temperature has been observed in the free-living amoeba Acanthamoeba castellanii. In this study we show that the enzyme can also be regulated by oxygen independently of temperatur...

journal_title：FEBS letters

authors： Thomas K,Rutter A,Suller M,Harwood J,Lloyd D

更新日期：1998-03-20 00:00:00

abstract：:The effect of the non-steroidal antioestrogens tamoxifen and toremifene on voltage-gated cationic currents was examined in primary cultures of rat hypothalamic neurones and the C1300 mouse neuroblastoma cell line using the whole-cell patch clamp technique. When applied to the external bathing solution both tamoxifen a...

journal_title：FEBS letters

authors： Hardy SP,deFelipe C,Valverde MA

更新日期：1998-09-04 00:00:00

abstract：:Human immunodeficiency virus type 1 (HIV-1) Rev is a small RNA-binding protein which is essential for viral replication. To investigate the structure of Rev we have mapped the binding sites of a panel of monoclonal antibodies (mAb) by protein footprinting and identified a mAb protecting amino acids within both the N- ...

journal_title：FEBS letters

authors： Jensen TH,Jensen A,Szilvay AM,Kjems J

更新日期：1997-09-01 00:00:00

abstract：:An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH ...

journal_title：FEBS letters

authors： Schulze S,Mant A,Kossmann J,Lloyd JR

更新日期：2004-05-07 00:00:00

abstract：:The conformational behavior of cyclic peptides of the amino acid sequence Cys-Phe/Ala-Pro-Ala-Cys has been investigated through the combined use of molecular simulation methods and NMR experiments to find models for beta-VIa turns of proteins. Both oxidized (cyclic) peptides and reduced (linear) forms were investigate...

journal_title：FEBS letters

authors： Weisshoff H,Frost K,Brandt W,Henklein P,Mügge C,Frömmel C

更新日期：1995-09-25 00:00:00

abstract：:To better understand the mechanism of intracellular Ca2+ mobilization, mouse oocytes were micro-injected with 'caged'-inositol-1,4,5 triphosphate caged-InsP3) together with the Ca2+ indicator Fluo-3 to directly induce and monitor Ca2+ redistribution. Photo-released InsP3 elicits [Ca2+]i changes exhibiting several kine...

journal_title：FEBS letters

authors： Peres A

更新日期：1990-11-26 00:00:00

abstract：:Pyrroloquinoline quinone (PQQ) has been claimed to be a component of glutamate decarboxylase from Escherichia coli on the basis of a frequently used procedure in which the protein is extracted with hexanol. We demonstrate that if pyridoxal phosphate (PLP) is not added during the preparation, the apoenzyme prepared fro...

journal_title：FEBS letters

authors： De Biase D,Maras B,John RA

更新日期：1991-01-14 00:00:00

abstract：:Viperin is a radical SAM enzyme that possesses antiviral properties against a broad range of enveloped viruses. Here, we describe the activity of human viperin with two molecules of the mevalonate pathway, geranyl pyrophosphate, and farnesyl pyrophosphate, involved in cholesterol biosynthesis. We postulate that the ra...

journal_title：FEBS letters

authors： Mikulecky P,Andreeva E,Amara P,Weissenhorn W,Nicolet Y,Macheboeuf P

更新日期：2018-01-01 00:00:00

abstract：:A macromolecular complex containing survival of motor neurons (SMN), the spinal muscular atrophy protein, and Gemin2-7 interacts with Sm proteins and snRNAs to carry out the assembly of these components into spliceosomal small nuclear ribonucleoproteins (snRNPs). Here we report the characterization of unr-interacting ...

journal_title：FEBS letters

authors： Carissimi C,Baccon J,Straccia M,Chiarella P,Maiolica A,Sawyer A,Rappsilber J,Pellizzoni L

更新日期：2005-04-25 00:00:00

abstract：:We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...

journal_title：FEBS letters

authors： Keppler M,Zegrocka O,Strekowski L,Fox KR

更新日期：1999-03-26 00:00:00

abstract：:Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...

journal_title：FEBS letters

authors： Esposito C,Carullo P,Pedone E,Graziano G,Del Vecchio P,Berisio R

更新日期：2010-03-19 00:00:00

abstract：:The binding of protein HU from Escherichia coli to nucleic acids was investigated by affinity chromatography under various conditions, by a nitrocellulose retention assay and by isopycnic centrifugations in metrizamide gradients. The results indicate that HU has a preference for binding to RNA and single-stranded DNA ...

journal_title：FEBS letters

authors： Holck A,Kleppe K

更新日期：1985-06-03 00:00:00

abstract：:Polysulfide is a bound sulfur species derived from endogenous H2S. When mouse neuroblastoma, Neuro2A cells were exposed to tert-butyl hydroperoxide after treatment with polysulfide, a significant decline in cell toxicity was observed. Rapid uptake of polysulfides induced translocation of Nrf2 into the nucleus, resulti...

journal_title：FEBS letters

authors： Koike S,Ogasawara Y,Shibuya N,Kimura H,Ishii K

更新日期：2013-11-01 00:00:00

abstract：:Adenosine can be metabolized by chicken erythrocytes as a carbon source maintaining ATP levels. In addition, it stimulates glucose uptake and lactate production. Adenosine effects do not involve binding to membrane receptors. They are dependent on the provision of a carbon source to glycolysis and correlate with the i...

journal_title：FEBS letters

authors： Espinet C,Bartrons R,Carreras J

更新日期：1989-11-20 00:00:00

abstract：:The tendency of DNA to form fibers upon condensation with counterions is reviewed. It is shown that chromatin fibers may acquire a relatively constant diameter of about 30 nm simply as an optimal size achieved upon neutralization of DNA, without requiring a repetitive internal structure. Thus the size of chromatin fib...

journal_title：FEBS letters

authors： Subirana JA

更新日期：1992-05-11 00:00:00

abstract：:Apoferritin has been selectively labeled with a maleimide nitroxide derivative at Cys-126, located in the hydrophilic 3-fold channels. Titration of this derivative with Fe(II), which gives rise to the initial Fe(III)-apoferritin complex, produces, at low metal-to-protein ratios, a decrease of the intensity of the labe...

journal_title：FEBS letters

authors： Desideri A,Stefanini S,Polizio F,Petruzzelli R,Chiancone E

更新日期：1991-08-05 00:00:00

abstract：:The O-chain polysaccharide (O-PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O-PS for structural analysis. High resolution (1)H and (13)C NMR and MALDI ...

journal_title：FEBS letters

authors： Larocque S,Brisson JR,Thérisod H,Perry MB,Caroff M

更新日期：2003-01-30 00:00:00

abstract：:The mitochondrial outer membrane contains a protein complex with at least eight subunits responsible for recognition and translocation of preproteins synthesized in the cytosol. Two subunits, the receptors Tom20 and Tom70, contain tetratrico peptide repeats that are thought to be involved in protein-protein interactio...

journal_title：FEBS letters

authors： Bömer U,Pfanner N,Dietmeier K

更新日期：1996-03-11 00:00:00

abstract：:The cyclic, imido acid containing pentapeptides cyclo(Asp-Trp-(NMe)Nle-Asp-Phe) (cpp[NMeNle(3)]) and cyclo(Asp-Trp-Pro-Asp-Phe) (cpp[Pro(3)]) have been investigated by 1H-NMR spectroscopy in DMSO and by restrained molecular dynamics methods. The spectra indicate the existence of at least four cis/trans isomers for cpp...

journal_title：FEBS letters

authors： Weisshoff H,Wieprecht T,Henklein P,Frömmel C,Antz C,Mügge C

更新日期：1996-06-03 00:00:00

abstract：:Ca(2+)-dependent protein kinases (CDPKs) play an essential role in plant Ca(2+)-mediated signal transduction. Twenty-nine CDPK genes have been identified in the rice genome through a complete search of genome and full-length cDNA databases. Eight of them were reported previously to be inducible by different stress sti...

journal_title：FEBS letters

authors： Wan B,Lin Y,Mou T

更新日期：2007-03-20 00:00:00