Cloning of a human liver UDP-glucose pyrophosphorylase cDNA by complementation of the bacterial galU mutation.

abstract：:The secretion mechanisms of cathepsin L from osteoclasts in the process of bone resorption were investigated. The increases in bone pit numbers formed take place by PTH addition in parallel with the increases of cathepsin L and/or L-like proteinase activities in the culture medium of bone cells, and these were suppres...

journal_title：FEBS letters

authors： Tagami K,Kakegawa H,Kamioka H,Sumitani K,Kawata T,Lenarcic B,Turk V,Katunuma N

更新日期：1994-04-11 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:Sialic acids comprise a family of terminal sugars essential for a variety of biological recognition systems. UDP-N-acetylglucosamine 2-epimerase catalyzes the first step of their biosynthesis. Periodate-oxidized UDP-N-acetylglucosamine, namely 2',3'-dialdehydo-UDP-alpha-D-N-acetylglucosamine, was found to be an effect...

journal_title：FEBS letters

authors： Blume A,Chen H,Reutter W,Schmidt RR,Hinderlich S

更新日期：2002-06-19 00:00:00

abstract：:The genetic modification of lysozyme was attempted to improve the bactericidal activity against Gram-negative bacteria E. coli. The different lengths of hydrophobic peptides were attached to the C-terminus of the hen egg white lysozyme to investigate the most effective length of the hydrophobic peptides for killing ba...

journal_title：FEBS letters

authors： Arima H,Ibrahim HR,Kinoshita T,Kato A

更新日期：1997-09-22 00:00:00

abstract：:The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

journal_title：FEBS letters

authors： Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

更新日期：1991-06-24 00:00:00

abstract：:The accumulation of amyloid-beta (Aβ) and tau aggregates is a pathological hallmark of Alzheimer's disease. Both polypeptides form fibrillar deposits, but several lines of evidence indicate that Aβ and tau form toxic oligomeric aggregation intermediates. Depleting such structures could thus be a powerful therapeutic s...

journal_title：FEBS letters

authors： Wobst HJ,Sharma A,Diamond MI,Wanker EE,Bieschke J

更新日期：2015-01-02 00:00:00

abstract：:Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...

journal_title：FEBS letters

authors： Rosenthal I,Krishna CM,Yang GC,Kondo T,Riesz P

更新日期：1987-09-28 00:00:00

abstract：:The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then ov...

journal_title：FEBS letters

authors： Adolph KW

更新日期：1984-01-09 00:00:00

abstract：:Activation of p53 plays a central role in the cell's response to various stress signals. We investigated whether p53 is activated upon disruption of actin microfilaments, caused by cytochalasin D (CD). We show that treatment with CD leads to accumulation of p53 in the cells and activation of p53-dependent transcriptio...

journal_title：FEBS letters

authors： Rubtsova SN,Kondratov RV,Kopnin PB,Chumakov PM,Kopnin BP,Vasiliev JM

更新日期：1998-07-03 00:00:00

abstract：:In this study, we demonstrated that laminin-1 (LM-1) induces neurite outgrowth and enhances the expression of neurofilament-L and MAP2 in human bone marrow mesenchymal stem cells (MSCs). The c-Jun transcription factor was strongly activated by LM-1 during neurite induction. Suppression of c-Jun inhibited the expressio...

journal_title：FEBS letters

authors： Mruthyunjaya S,Rumma M,Ravibhushan G,Anjali S,Padma S

更新日期：2011-06-23 00:00:00

abstract：:Isolation and characterization of the cDNA coding for the 216-residue Xenopus laevis prion protein is reported. Existence of this protein in amphibians was suggested by an EST fragment (accession number BG813008), while a conclusive demonstration is presented here. This protein exhibits a higher identity level to avia...

journal_title：FEBS letters

authors： Strumbo B,Ronchi S,Bolis LC,Simonic T

更新日期：2001-11-16 00:00:00

abstract：:We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-alpha)- and receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly in...

journal_title：FEBS letters

authors： Yamaguchi N,Kukita T,Li YJ,Kamio N,Fukumoto S,Nonaka K,Ninomiya Y,Hanazawa S,Yamashita Y

更新日期：2008-02-06 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

journal_title：FEBS letters

authors： McIntyre GD,Chaudhuri S,Coggins JR

更新日期：1984-10-01 00:00:00

abstract：:In the filamentous fungus Neurospora, the production of dsRNA can elicit a dsRNA-induced transcriptional response similar to the interferon response in vertebrates. However, how fungi sense the expression of dsRNA and activate gene expression is unknown. In this study, we established a dsRNA response reporter system i...

journal_title：FEBS letters

authors： Li X,Shen Y,Sun J,Wang B,He Q

更新日期：2011-03-23 00:00:00

abstract：:Macrophages are abundant in the tumor microenvironment. They are highly plastic and able to acquire pro-tumoral phenotypes in response to microenvironmental stimuli. When we treated RAW 264.7 macrophages with inhibitors of various oncogenic pathways, we found that the focal adhesion kinase (FAK) inhibitors PF573228 an...

journal_title：FEBS letters

authors： He X,Chen X,Li B,Ji J,Chen S

更新日期：2017-12-01 00:00:00

abstract：:Treatment of neonatal rat islets of Langerhans with combined cytokines (interleukin-1beta 10(-10) M, tumour necrosis factor-alpha 10(-10) M, interferon-gamma 5 U/ml) led to extensive cell death, which was potentiated by Fas activation with the anti-Fas cytolytic antibody JO2. Pre-treatment with insulin (25 ng/ml) or i...

journal_title：FEBS letters

authors： Harrison M,Dunger AM,Berg S,Mabley J,John N,Green MH,Green IC

更新日期：1998-09-18 00:00:00

abstract：:Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [3H]L-glutamate by a Na(+)-dependent uptake process strongly inhibited by several glutamate/aspartate analogues including D- and L-aspartate, D- and L-threo-3-hydroxyaspartate and L-trans-pyrrolidine-2...

journal_title：FEBS letters

authors： Balcar VJ,Shen J,Bao S,King NJ

更新日期：1994-02-14 00:00:00

abstract：:We have compared the urea stability of the human aromatic amino acid hydroxylases (AAAHs), key enzymes involved in neurotransmitter biosynthesis and amino acid homeostasis. Tyrosine-, tryptophan- and phenylalanine hydroxylase (TH, TPH and PAH, respectively) were transiently activated at low urea concentrations and rap...

journal_title：FEBS letters

authors： Kleppe R,Haavik J

更新日期：2004-05-07 00:00:00

abstract：:Ribonucleoprotein complexes, which contain mRNAs and their regulator proteins, carry out post-transcriptional control of gene expression. The function of many RNA-binding proteins depends on their association with cofactors. Here, we use a genomic approach to identify transcripts associated with DLC-1, a protein previ...

journal_title：FEBS letters

authors： Day NJ,Ellenbecker M,Voronina E

更新日期：2018-11-01 00:00:00

abstract：:Three inositol-containing sialyloligosaccharides were isolated from normal human urine. Structural studies including gas-liquid chromatography of mono- and disaccharide derivatives, methylation analysis, mass spectrometry and glycosidase treatments indicated the structure NeuAc (alpha 2-3)Gal(beta 1-0)scyllo-inositol ...

journal_title：FEBS letters

authors： Parkkinen J

更新日期：1983-10-31 00:00:00

abstract：:Studies of mutants of the yeast Saccharomyces cerevisiae have led to the identification of genes required for high affinity iron uptake. Reduction of iron (III) outside the cell is accomplished by means of reductases encoded by FRE1 and FRE2, homologues of the gp91-phox component of the oxygen reductase of human granu...

journal_title：FEBS letters

authors： Klausner RD,Dancis A

更新日期：1994-11-28 00:00:00

abstract：:Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance sp...

journal_title：FEBS letters

authors： Nabben M,Hoeks J,Briedé JJ,Glatz JF,Moonen-Kornips E,Hesselink MK,Schrauwen P

更新日期：2008-12-24 00:00:00

abstract：:Malignant mesothelioma (MM) is a fatal type of cancer. We studied the role of the redox-active protein thioredoxin-1 (Trx-1) in apoptosis induced in MM cells and their non-malignant counterparts (Met-5A) by alpha-tocopheryl succinate (alpha-TOS) and TNF-related apoptosis-inducing ligand (TRAIL). MM cells were suscepti...

journal_title：FEBS letters

authors： Freeman RE,Neuzil J

更新日期：2006-05-15 00:00:00

abstract：:Recent three-dimensional structures of helical membrane proteins present new challenges for the prediction of structure from amino acid sequence. Membrane proteins reside stably in a thermodynamic free energy minimum after release into the membrane's bilayer fabric from the translocon complex. This means that structur...

journal_title：FEBS letters

authors： White SH

更新日期：2003-11-27 00:00:00

abstract：:Six charged amino acid residues located in the ectodomain of the full-length type I transforming growth factor (TGF)-beta receptor were individually mutated to alanine. Mutation of residues D47, D98, K102 and E104 resulted in functionally impaired receptors as demonstrated by a marked decrease in ligand-dependent sign...

journal_title：FEBS letters

authors： Guimond A,Sulea T,Zwaagstra JC,Ekiel I,O'Connor-McCourt MD

更新日期：2002-02-27 00:00:00

abstract：:The last enzyme (LytB) of the methylerythritol phosphate pathway for isoprenoid biosynthesis catalyzes the reduction of (E)-4-hydroxy-3-methylbut-2-enyl diphosphate into isopentenyl diphosphate and dimethylallyl diphosphate. This enzyme possesses a dioxygen-sensitive [4Fe-4S] cluster. This prosthetic group was charact...

journal_title：FEBS letters

authors： Wolff M,Seemann M,Tse Sum Bui B,Frapart Y,Tritsch D,Garcia Estrabot A,Rodríguez-Concepción M,Boronat A,Marquet A,Rohmer M

更新日期：2003-04-24 00:00:00

abstract：:The bacterial cell wall muramyl dipeptides MDP and glucosaminyl-MDP (GMDP) are powerful immunostimulators but their binding target remains controversial. We previously reported expression cloning of GMDP-binding polypeptides and identification of Y-box protein 1 (YB-1) as their sole target. Here we show specific bindi...

journal_title：FEBS letters

authors： Laman AG,Lathe R,Savinov GV,Shepelyakovskaya AO,Boziev KhM,Baidakova LK,Chulin AN,Brovko FA,Svirshchevskaya EV,Kotelevtsev Y,Eliseeva IA,Guryanov SG,Lyabin DN,Ovchinnikov LP,Ivanov VT

更新日期：2015-07-08 00:00:00

abstract：:Galanin mediates diverse physiological functions in digestive, endocrine, and central nervous systems through G-protein-coupled receptors. Two galanin receptors have been cloned but the gene structures are unknown. We report genomic and cDNA cloning of the mouse GalR1 galanin receptor and demonstrate that the coding s...

journal_title：FEBS letters

authors： Wang S,He C,Maguire MT,Clemmons AL,Burrier RE,Guzzi MF,Strader CD,Parker EM,Bayne ML

更新日期：1997-07-14 00:00:00

abstract：:The present study describes a significant amino acid sequence homology between neuronal Cdk5 activator (nck5a) and an open reading frame of an unknown gene on the yeast S. cerevisiae chromosome III. A cDNA encoding a 25 kDa fragment of this yeast protein, the region containing homologous sequence to nck5a was cloned a...

journal_title：FEBS letters

authors： Huang QQ,Lee KY,Wang JH

更新日期：1996-01-02 00:00:00