Different stabilities and denaturation pathways for structurally related aromatic amino acid hydroxylases.

abstract：:Anomalously high values of photoinduced absorption changes were revealed in the antenna of photosynthetic purple bacteria. They were found to be 4-16 times greater at the bleaching peak of the antenna than at the bleaching peak of the BChl dimer of the reaction center. This is direct proof of excitation delocalization...

journal_title：FEBS letters

authors： Novoderezhkin VI,Razjivin AP

更新日期：1995-07-17 00:00:00

abstract：:The effect of oxLDL on CD36 expression has been assessed in preadipocytes induced to differentiate. Novel evidence is provided that oxLDL induce a peroxisome proliferator-activated receptor gamma-independent CD36 overexpression, by up-regulating nuclear factor erythroid 2 (NF-E2)-related factor 2 (Nrf2). The nuclear t...

journal_title：FEBS letters

authors： D'Archivio M,Scazzocchio B,Filesi C,Varì R,Maggiorella MT,Sernicola L,Santangelo C,Giovannini C,Masella R

更新日期：2008-06-25 00:00:00

abstract：:Little is known about the mechanisms underlying calcium-induced Ca2+ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca2+, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with 45Ca2+ showed voltage- and...

journal_title：FEBS letters

authors： Bewell MA,Maathuis FJ,Allen GJ,Sanders D

更新日期：1999-09-10 00:00:00

abstract：:To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subun...

journal_title：FEBS letters

authors： Shim EK,Moon CS,Lee GY,Ha YJ,Chae SK,Lee JR

更新日期：2004-09-24 00:00:00

abstract：:Most geckos are nocturnal forms and possess rod retinas, but some diurnal genera have pure-cone retinas. We isolated cDNAs encoding the diurnal gecko opsins, dg1 and dg2, similar to nocturnal gecko P521 and P467, respectively. Despite the large morphological differences between the diurnal and nocturnal gecko photorec...

journal_title：FEBS letters

authors： Taniguchi Y,Hisatomi O,Yoshida M,Tokunaga F

更新日期：1999-02-19 00:00:00

abstract：:Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...

journal_title：FEBS letters

authors： Satoh T,Sumiyoshi A,Yagi-Utsumi M,Sakata E,Sasakawa H,Kurimoto E,Yamaguchi Y,Li W,Joazeiro CA,Hirokawa T,Kato K

更新日期：2014-11-28 00:00:00

abstract：:The voltage-dependent calcium channel (VDCC) in skeletal muscle probably plays a key role in transducing membrane charge movement to the calcium release channel. We report here that the expression of VDCC alpha 1 and alpha 2 mRNAs is developmentally regulated in differentiating C2C12 myogenic cells. The alpha 1 mRNA i...

journal_title：FEBS letters

authors： Varadi G,Orlowski J,Schwartz A

更新日期：1989-07-03 00:00:00

abstract：:The ubiquitin-editing enzyme A20 suppresses nuclear factor-κB (NF-κB) activation and tumor necrosis factor-α (TNF-α)-induced apoptosis in a deubiquitinating and ubiquitin ligase activity-dependent manner. Although recent studies revealed that A20 regulates NF-κB independently of its enzymatic activity through its seve...

journal_title：FEBS letters

authors： Yamaguchi N,Yamaguchi N

更新日期：2015-05-22 00:00:00

abstract：:Acylphosphatase (AcP) activity in prokaryotes was classically attributed to some aspecific acid phosphatases. We identified an open reading frame for a putative AcP in the b0968 Escherichia coli gene and purified the recombinant enzyme after checking by RT-PCR that it was indeed expressed. EcoAcP has a predicted typic...

journal_title：FEBS letters

authors： Ramazzotti M,Parrini C,Stefani M,Manao G,Degl'Innocenti D

更新日期：2006-12-22 00:00:00

abstract：:Tau is reversibly hyperphosphorylated in the mouse brain by starvation or cold water swimming. Here, we report tau phosphorylation in the hippocampus of normal mouse after ether anesthesia, known to trigger typical stress reactions. Robust phosphorylation of tau was observed immediately and 10min after ether vapor exp...

journal_title：FEBS letters

authors： Ikeda Y,Ishiguro K,Fujita SC

更新日期：2007-03-06 00:00:00

abstract：:We solved the crystal structure of a functionally uncharacterized protein, HP0902, from Helicobacter pylori. Its structure demonstrated an all-β cupin fold that cannot bind metal ions due to the absence of a metal-binding histidine that is conserved in many metallo-cupins. In contrast, isothermal titration calorimetry...

journal_title：FEBS letters

authors： Sim DW,Kim JH,Kim HY,Jang JH,Lee WC,Kim EH,Park PJ,Lee KH,Won HS

更新日期：2016-09-01 00:00:00

abstract：:Haloalkane dehalogenase hydrolyses various 1-halo-n-alkanes to the corresponding alcohols by covalent catalysis with formation of an alkyl-enzyme intermediate. The carboxylate function of the nucleophilic aspartate (Asp-124) that displaces the halogen during formation of the intermediate was changed to an amide by sit...

journal_title：FEBS letters

authors： Pries F,Kingma J,Janssen DB

更新日期：1995-01-23 00:00:00

abstract：:Human aminopeptidase N carries an apical sorting signal on its ectodomain necessary for its correct transport to the apical membrane in Madin-Darby canine kidney cells. To determine whether the apical sorting signal is localized in the serine/threonine rich stalk or in the catalytic head group, anchor/stalk-minus amin...

journal_title：FEBS letters

authors： Vogel LK,Norén O,Sjöström H

更新日期：1992-08-10 00:00:00

abstract：:The functionality of the disintegrin-like/cysteine-rich domains of snake venom metalloproteinases (SVMPs) has been shown to reside in the cysteine-rich region, which can interact with VWA-containing proteins. Recently, the hyper-variable region (HVR) of the cysteine-rich domain was suggested to constitute a potential ...

journal_title：FEBS letters

authors： Menezes MC,Paes Leme AF,Melo RL,Silva CA,Della Casa M,Bruni FM,Lima C,Lopes-Ferreira M,Camargo AC,Fox JW,Serrano SM

更新日期：2008-11-26 00:00:00

abstract：:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

journal_title：FEBS letters

authors： Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

更新日期：2011-03-23 00:00:00

abstract：:A new phagemid cloning vector for positive selection of recombinants, pBa-7, was constructed which contains an active barnase gene encoding the cytotoxic ribonuclease from Bacillus amyloliquefaciens, under control of the lac promoter. PBa-7 is a derivative of the high-copy number pBluescript II KS+ phagemid in which t...

journal_title：FEBS letters

authors： Yazynin S,Lange H,Mokros T,Deyev S,Lemke H

更新日期：1999-06-11 00:00:00

abstract：:Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) ...

journal_title：FEBS letters

authors： Shuvaev VV,Siest G

更新日期：1996-03-25 00:00:00

abstract：:Colneleic acid (9-[1'(E),3'(Z)-nonadienyloxy]-8(E)-nonenoic acid) is produced from linoleic acid by the sequential action of 9-lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemica...

journal_title：FEBS letters

authors： Fauconnier ML,Williams TD,Marlier M,Welti R

更新日期：2003-03-13 00:00:00

abstract：:Myogenin, a member of the MyoD family which governs skeletal muscle differentiation, was identified as a pair of phosphorylated bands on SDS-PAGE during myogenesis. The slow migrating form was found to be hyperphosphorylated myogenin. In vitro phosphorylation by CDC2 kinase caused a prominent reduction in electrophore...

journal_title：FEBS letters

authors： Hashimoto N,Ogashiwa M,Okumura E,Endo T,Iwashita S,Kishimoto T

更新日期：1994-09-26 00:00:00

abstract：:The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250-555 positioned within the c...

journal_title：FEBS letters

authors： Olsen J,Cowell GM,Kønigshøfer E,Danielsen EM,Møller J,Laustsen L,Hansen OC,Welinder KG,Engberg J,Hunziker W

更新日期：1988-10-10 00:00:00

abstract：:10Sa RNA is a bacterial small stable RNA, in which the 5'- and 3'-end sequences are folded into a tRNA-like structure. The RNA accepts alanine in vitro, and interacts with 70S ribosomes in the cells. In this study, we examined the ribosome-binding properties of Escherichia coli 10Sa RNA in vivo, and found that the ami...

journal_title：FEBS letters

authors： Tadaki T,Fukushima M,Ushida C,Himeno H,Muto A

更新日期：1996-12-16 00:00:00

abstract：:Endoplasmic reticulum stress (ER-stress) is associated with ataxia telangiectasia mutated (ATM) gene. We present here conclusive data showing that ATM blocks ER-stress induced by tunicamycin or ionizing radiation (IR). X-box protein-1 (XBP-1) splicing, GRP78 expression and caspase-12 activation were increased by tunic...

journal_title：FEBS letters

authors： He L,Kim SO,Kwon O,Jeong SJ,Kim MS,Lee HG,Osada H,Jung M,Ahn JS,Kim BY

更新日期：2009-03-04 00:00:00

abstract：:In human pancreatic islets an increase in the glucose concentration from 3 to 20 mM raised the free cytoplasmic Ca2+ concentration [( Ca2+]i), an effect being reversible upon withdrawal of the sugar. Depolarization with a high concentration of K+ or the sulphonylurea tolbutamide also raised [Ca2+]i. Addition of extrac...

journal_title：FEBS letters

authors： Kindmark H,Köhler M,Nilsson T,Arkhammar P,Wiechel KL,Rorsman P,Efendić S,Berggren PO

更新日期：1991-10-21 00:00:00

abstract：:A mutant of Escherichia coli has been described that produces an altered form of penicillin-binding protein 5 which still binds penicillin but is unable to catalyse the release of the bound penicilloyl moiety. We show that the mutation is caused by a single nucleotide transition that results in a change from glycine a...

journal_title：FEBS letters

authors： Broome-Smith J,Spratt BG

更新日期：1984-01-09 00:00:00

abstract：:We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin K mRNA for studies on bone turnover. The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences. Analysis of a panel of mouse tissues for tissue di...

journal_title：FEBS letters

authors： Rantakokko J,Aro HT,Savontaus M,Vuorio E

更新日期：1996-09-16 00:00:00

abstract：:Quantitative modeling of intracellular processes often requires information about intracellular rate constants as well as the concentrations of low abundance species in individual cells. Single molecule imaging techniques offer not only new ways for obtaining such information but also the possibilities to test model-b...

journal_title：FEBS letters

authors： Li GW,Elf J

更新日期：2009-12-17 00:00:00

abstract：:Purified plasma membrane vesicles isolated from multidrug-resistant human KB-V1 cells accumulate [3H]vinblastine in an energy-dependent manner. The accumulation of [3H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most effic...

journal_title：FEBS letters

authors： Lelong IH,Padmanabhan R,Lovelace E,Pastan I,Gottesman MM

更新日期：1992-06-15 00:00:00

abstract：:The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1...

journal_title：FEBS letters

authors： Sachinidis A,Weisser P,Ko Y,Schulte K,Meyer zu Brickwedde K,Neyses L,Vetter H

更新日期：1992-11-23 00:00:00

abstract：:The function of the coenzyme Q (CoQ) pool in the inner mitochondrial membrane is reviewed in view of recent findings suggesting a supramolecular organization of the mitochondrial respiratory complexes. In spite of the structural evidence for preferential aggregations of the inner membrane components, most kinetic evid...

journal_title：FEBS letters

authors： Lenaz G

更新日期：2001-12-07 00:00:00

abstract：:Calpastatin molecule contains four repeated inhibition domains, each having highly conserved internal regions A, B and C. The synthetic oligopeptides of regions A and C had no calpain inhibition activity while region B oligopeptide showed weak inhibition activity. Real-time biomolecular interaction analysis using a BI...

journal_title：FEBS letters

authors： Takano E,Ma H,Yang HQ,Maki M,Hatanaka M

更新日期：1995-03-27 00:00:00