Interaction of 10Sa RNA with ribosomes in Escherichia coli.

abstract：:The β-xylosidase B from Bifidobacterium adolescentis ATCC15703 belongs to the newly characterized family 120 of glycoside hydrolases. In order to investigate its catalytic mechanism, an extensive kinetic study of the wild-type enzyme and mutants targeting the three highly conserved residues Asp(393), Glu(416) and Glu(...

journal_title：FEBS letters

authors： Cecchini DA,Fauré R,Laville E,Potocki-Veronese G

更新日期：2015-10-07 00:00:00

abstract：:Novel catechin derivatives obtained from grape procyanidins and l-cysteine scavenge free radicals by hydrogen atom donation, rather than electron transfer, and reduce cell viability in A375 and M21 melanoma cells. In particular, 4beta-(S-cysteinyl)epicatechin 3-O-gallate has a free radical scavenging capacity as stron...

journal_title：FEBS letters

authors： Lozano C,Torres JL,Julià L,Jimenez A,Centelles JJ,Cascante M

更新日期：2005-08-15 00:00:00

abstract：:We have previously published a simple kinetic model to analyse possible pitfalls in kinetic measurements of H+/O ratios in mitochondria [(1984) FEBS Lett. 178, 187-192]. While this model demonstrated how relative electrode response times may affect the results, it did not adequately describe the kinetics of proton bac...

journal_title：FEBS letters

authors： Krab K,Wikström M

更新日期：1986-06-09 00:00:00

abstract：:Analysis of nascent gamma B-crystallin peptides accumulating during in vitro translation in a rabbit reticulocyte lysate cell-free system was carried out. As a consequence of the irregular distribution of rare codons along the polypeptide chain of gamma B-crystallin, translation of the two-domain protein is a non-unif...

journal_title：FEBS letters

authors： Komar AA,Jaenicke R

更新日期：1995-12-04 00:00:00

abstract：:The phosphatidylinositol 4,5-bisphosphate (PtdIns-P2)-regulated actin-binding protein gelsolin and most phosphoinositide-specific phospholipases C (PLCs) comprise a basic amino acid motif ((K/R)xxxKxK(K/R); x denotes any amino acid) which was previously suggested to represent a PtdInsP2-binding site commonly present i...

journal_title：FEBS letters

authors： Simões AP,Camps M,Schnabel P,Gierschik P

更新日期：1995-05-29 00:00:00

abstract：:We have generated a mouse model for hepatic ischemia in which surgical subcutaneous transposition of the spleen allows hepatic ischemia to be applied without affecting other tissues. Using this mouse model we investigated the relationship between the length of ischemic periods in the liver and subsequent liver functio...

journal_title：FEBS letters

authors： Onishi I,Tani T,Hashimoto T,Shimizu K,Yagi M,Yamamoto K,Yoshioka K

更新日期：1997-12-29 00:00:00

abstract：:We report the identification, gene expression and biological activity of two parathyroid hormones (PTH; PTHA and PTHB), two PTH-related peptides (PTHrP; PTHrPA and PTHrPB) and a PTH-like ligand (PTH-L) with hybrid characteristics in puffer fishes (Takifugu rubripes and Tetraodon fluviatilis). Experimental data are con...

journal_title：FEBS letters

authors： Canario AV,Rotllant J,Fuentes J,Guerreiro PM,Rita Teodósio H,Power DM,Clark MS

更新日期：2006-01-09 00:00:00

abstract：:Addition of hydrocortisone to the rat mammary gland myoepithelial cell line, G4.2.3, induces the synthesis and secretion of alpha 2-macroglobulin. Interleukin-1 beta, interleukin-6 and transforming growth factor-beta synergize with hydrocortisone, increasing the synthesis of alpha 2-macroglobulin 2- to 4-fold, althoug...

journal_title：FEBS letters

authors： Warburton MJ,Gusterson BA,O'Hare MJ

更新日期：1993-10-11 00:00:00

abstract：:Adrenal medullary homogenates and chromaffin granule membranes were separated by SDS-polyacrylamide gel electrophoresis and GTP-binding proteins detected using [alpha-32P]GTP binding to nitrocellulose blots. Four GTP-binding polypeptides of 24, 22, 20 and 18 kDa were routinely found in medullary homogenates and all we...

journal_title：FEBS letters

authors： Burgoyne RD,Morgan A

更新日期：1989-03-13 00:00:00

abstract：:Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of Mr 37,000, 40,000 and 45,000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal...

journal_title：FEBS letters

authors： Longbottom D,van Heyningen S

更新日期：1990-10-15 00:00:00

abstract：:Mitochondrial translocation of ceramides triggers Bax-dependent apoptosis. To elucidate how ceramides activate Bax and commit cells to death, we developed a switchable version of the ceramide transfer protein CERT, sCERT. Upon its drug-induced recruitment to mitochondria, sCERT retains the ability to bind VAP proteins...

journal_title：FEBS letters

authors： Jain A,Dadsena S,Holthuis JCM

更新日期：2020-11-01 00:00:00

abstract：:Transport of the precursor for carbamoyl-phosphate synthetase I into mitochondria in Reuber hepatoma H-35 cells was inhibited by adding monensin or nigericin to the culture medium at a concentration of 0.5 microM, and the enzyme precursor accumulated, mainly in the cytosolic fraction. Accumulated precursor was degrade...

journal_title：FEBS letters

authors： Kitagawa Y,Murakami A,Sugimoto E

更新日期：1984-01-02 00:00:00

abstract：:The yeast integral plasma membrane protein Ist2 belongs to a group of membrane proteins which are synthesized from localized mRNAs. The protein reaches the plasma membrane via the ER on a route operating independently of the classical secretory pathway. We have identified a complex peptide-sorting signal located at th...

journal_title：FEBS letters

authors： Franz A,Maass K,Seedorf M

更新日期：2007-02-06 00:00:00

abstract：:Three homologous aspartate aminotransferases with virtually identical spatial structures and pairwise amino acid sequence identities of > 40% differ markedly with respect to the yield of renaturation upon dilution from 6 M guanidine hydrochloride (mitochondrial < cytosolic < Escherichia coli). The enzymes also respond...

journal_title：FEBS letters

authors： Widmann M,Christen P

更新日期：1995-12-27 00:00:00

abstract：:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

journal_title：FEBS letters

authors： Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

更新日期：2011-03-23 00:00:00

abstract：:Intravenous injections of 25.5 nmol rat amylin into fasted anesthetized rats caused a rapid increase in plasma lactate followed by an increase in plasma glucose; there was a transient fall in blood pressure. Subcutaneous injection of 25.5 nmol amylin also caused increases in lactate and glucose but did not change bloo...

journal_title：FEBS letters

authors： Young AA,Wang MW,Cooper GJ

更新日期：1991-10-07 00:00:00

abstract：:Oscillations of free intracellular calcium [Ca2+]i were seen in individual Jurkat cells as response to a 50 Hz, 0.15 mT magnetic field (MF). In contrast, a CD45-deficient Jurkat cell line was unable to respond to MF stimulation. The phosphatase activity of CD45 has been implicated to regulate p56lck tyrosine kinase ac...

journal_title：FEBS letters

authors： Lindström E,Berglund A,Mild KH,Lindström P,Lundgren E

更新日期：1995-08-14 00:00:00

abstract：:The eukaryotic transcription factor NF-kappaB is activated by a large variety of stimuli. We have recently shown that ER stress, caused by an aberrant accumulation of membrane proteins within this organelle, also activates NF-kappaB. Here, we show that activation of NF-kappaB by ER stress requires an increase in the i...

journal_title：FEBS letters

authors： Pahl HL,Baeuerle PA

更新日期：1996-08-26 00:00:00

abstract：:The inositol (1,4,5)-trisphosphate receptor (InsP3R) is an intracellular calcium release channel that plays a crucial role in cell signaling. In Drosophila melanogaster, a single InsP3R gene (itpr) encodes a protein (DmInsP3R) that is approximately 60% conserved with mammalian InsP3Rs. The functional properties of wil...

journal_title：FEBS letters

authors： Srikanth S,Wang Z,Hasan G,Bezprozvanny I

更新日期：2004-09-24 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:Using specific monoclonal and polyclonal antibodies, we have analyzed protein kinase C alpha and beta isozyme expression in human T cells from peripheral blood (PB) and from thymus. While the PKC beta isozyme was present in all T cell sub-types isolated from both PB and thymus, the alpha isozyme was found only in sing...

journal_title：FEBS letters

authors： Tarantino N,Debré P,Körner M

更新日期：1994-02-07 00:00:00

abstract：:Recent studies have shown that p21-activated kinase 1 (Pak1) phosphorylates estrogen receptor-alpha (ER alpha) at Ser 305 and also promotes its transactivation function. Here, we sought to investigate whether substitution of serine 305 in ER with glutamic acid (ER alpha-S305E), which mimics the phosphorylation state, ...

journal_title：FEBS letters

authors： Balasenthil S,Barnes CJ,Rayala SK,Kumar R

更新日期：2004-06-04 00:00:00

abstract：:The up-regulation of HER2/neu is associated with human malignancies and is a useful target for developing anticancer drugs. Overexpression of human manganese superoxide dismutase (MnSOD) has been demonstrated to effectively suppress various carcinoma cells, including breast carcinomas, in vitro and in vivo. This study...

journal_title：FEBS letters

authors： Chuang TC,Liu JY,Lin CT,Tang YT,Yeh MH,Chang SC,Li JW,Kao MC

更新日期：2007-09-18 00:00:00

abstract：:Cytosolic sialidase Neu2 has been implicated in myoblast differentiation. Here we observed a significant upregulation of Neu2 expression during differentiation of murine C2C12 myoblasts. This was evidenced both as an increase in Neu2 mRNA steady-state levels and in the cytosolic sialidase enzymatic activity. To unders...

journal_title：FEBS letters

authors： Fanzani A,Giuliani R,Colombo F,Zizioli D,Presta M,Preti A,Marchesini S

更新日期：2003-07-17 00:00:00

abstract：:In the present study, we investigated the roles and molecular mechanisms of miR-320a in human nasopharyngeal carcinoma (NPC). miR-320a expression was strongly reduced in NPC tissues and cell lines. Overexpression of miR-320a significantly suppressed NPC cell growth, migration, invasion and tumor growth in a xenograft ...

journal_title：FEBS letters

authors： Qi X,Li J,Zhou C,Lv C,Tian M

更新日期：2014-10-16 00:00:00

abstract：:The amino acid residue Asn141 of the restriction endonuclease EcoRI was proposed to make three hydrogen bonds to both adenine residues within the recognition sequence -GAATTC-. We have mutated Asn141 to alanine, aspartate, serine, and tyrosine. Only the serine mutant is active under normal buffer conditions although 1...

journal_title：FEBS letters

authors： Fritz A,Küster W,Alves J

更新日期：1998-10-30 00:00:00

abstract：:Blood reprogramming, in which induced pluripotent stem cells (iPSCs) are derived from haematopoietic lineages, has rapidly advanced over the past decade. Since the first report using human blood, haematopoietic cell types from various sources, such as the peripheral bone marrow and cord blood, have been successfully r...

journal_title：FEBS letters

authors： Chen Y,Yi Y,Xu J,Chan WK,Loh YH

更新日期：2019-12-01 00:00:00

abstract：:Apomyoglobin was found to break down phosphatidylcholine vesicles to form a micellar complex at pH 4. Size reduction during this process was observed by measuring light scattering at 400 nm and by passing the complex through a gel-filtration column. Micellar complex formation was confirmed by electron microscopy. ...

journal_title：FEBS letters

authors： Lee JW,Kim H

更新日期：1988-12-05 00:00:00

abstract：:pMNEI, a single chain sweet protein related to monellin, has been studied by means of 1H NMR at 500 MHz. A partial sequential assignment performed by means of the MCD method allowed the determination of the secondary structure of a large portion of the beta-sheet of pMNEI that contains a likely 'sweet finger': the loo...

journal_title：FEBS letters

authors： Tancredi T,Iijima H,Saviano G,Amodeo P,Temussi PA

更新日期：1992-09-21 00:00:00

abstract：:The NusB protein is involved in transcriptional regulation in bacteriophage lambda. NusB binds to the RNA form of the nut site and along with N, NusA, NusE and NusG, stabilizes the RNA polymerase transcription complex and allows stable, persistent antitermination. NusB contains a 10 residue Arg-rich RNA-binding motif ...

journal_title：FEBS letters

authors： Altieri AS,Mazzulla MJ,Zhou H,Costantino N,Court DL,Byrd RA

更新日期：1997-09-29 00:00:00