Re-entering the pluripotent state from blood lineage: promises and pitfalls of blood reprogramming.

abstract：:There are two common forms of NRH-quinone oxidoreductase 2 (NQO2) in the human population resulting from SNP rs1143684. One has phenylalanine at position 47 (NQO2-F47) and the other leucine (NQO2-L47). Using recombinant proteins, we show that these variants have similar steady state kinetic parameters, although NQO2-L...

journal_title：FEBS letters

authors： Megarity CF,Gill JR,Caraher MC,Stratford IJ,Nolan KA,Timson DJ

更新日期：2014-05-02 00:00:00

abstract：:Astrocyte-elevated gene-1 (AEG-1) positively regulates tumor progression and metastasis. Here, we document that AEG-1 upregulates transcription of the membrane protein tetraspanin 8 (TSPAN8). Knocking down TSPAN8 in AEG-1-overexpressing human hepatocellular carcinoma (HCC) cells markedly inhibited invasion and migrati...

journal_title：FEBS letters

authors： Akiel MA,Santhekadur PK,Mendoza RG,Siddiq A,Fisher PB,Sarkar D

更新日期：2016-08-01 00:00:00

abstract：:Thymocytes previously loaded with quin 2 were rapidly permeabilized by the photon activation of erythrosin and the rate of permeabilization monitored by measuring fluorimetrically the increasing saturation of quin 2 with calcium. The extent of permeabilization was assessed also by the loss of [3H]quin 2 from the thymo...

journal_title：FEBS letters

authors： Yonuschot G,Matthews EK,Corps AN,Metcalfe JC

更新日期：1987-03-23 00:00:00

abstract：:A high intake of dietary fat has been suggested to diminish mitochondrial functioning in skeletal muscle, possibly attributing to muscular fat accumulation. Here we show however, that an 8-week high-fat dietary intervention did not affect intrinsic functioning of rat skeletal muscle mitochondria assessed by respiromet...

journal_title：FEBS letters

authors： Hoeks J,Briedé JJ,de Vogel J,Schaart G,Nabben M,Moonen-Kornips E,Hesselink MK,Schrauwen P

更新日期：2008-02-20 00:00:00

abstract：:The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this ...

journal_title：FEBS letters

authors： Ledent P,Duez C,Vanhove M,Lejeune A,Fonzé E,Charlier P,Rhazi-Filali F,Thamm I,Guillaume G,Samyn B,Devreese B,Van Beeumen J,Lamotte-Brasseur J,Frère JM

更新日期：1997-08-18 00:00:00

abstract：:Ribosomal protection proteins (RPPs) confer bacterial resistance to tetracycline by releasing this antibiotic from ribosomes stalled in protein synthesis. RPPs share structural similarity to elongation factor G (EF-G), which promotes ribosomal translocation during normal protein synthesis. We constructed and functiona...

journal_title：FEBS letters

authors： Thakor NS,Nechifor R,Scott PG,Keelan M,Taylor DE,Wilson KS

更新日期：2008-04-16 00:00:00

abstract：:Three novel neuropeptides, isolated from ganglia of the African giant snail, Achatina fulica, were named WWamide-1, -2 and -3. These substances were biologically active heptapeptide amides with a Trp residue at both the N- and C-termini. WWamide-1, which displayed an inhibitory activity on a central neuron of the snai...

journal_title：FEBS letters

authors： Minakata H,Ikeda T,Muneoka Y,Kobayashi M,Nomoto K

更新日期：1993-05-24 00:00:00

abstract：:The effect of the uremic solute indoxyl sulfate (IS) on scavenging superoxide anion radicals (O(2)(*-)) generated from both the xanthine/xanthine oxidase (X/XO) system and activated neutrophils was investigated by electron paramagnetic resonance spectroscopy, combined with 2-ethoxycarbonyl-2-methyl-3,4-dihydro-2H-pyrr...

journal_title：FEBS letters

authors： Miyamoto Y,Iwao Y,Tasaki Y,Sato K,Ishima Y,Watanabe H,Kadowaki D,Maruyama T,Otagiri M

更新日期：2010-07-02 00:00:00

abstract：:We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the ...

journal_title：FEBS letters

authors： Prescott M,Lourbakos A,Bateson M,Boyle G,Nagley P,Devenish RJ

更新日期：1997-07-07 00:00:00

abstract：:Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...

journal_title：FEBS letters

authors： Kwon JY,Kim-Ha J,Lee BJ,Lee J

更新日期：2001-11-23 00:00:00

abstract：:We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Tran...

journal_title：FEBS letters

authors： Ko HM,Jung HH,Seo KH,Kang YR,Kim HA,Park SJ,Lee HK,Im SY

更新日期：2006-05-29 00:00:00

abstract：:The C-termini of p66 and p51 forms of HIV-1 reverse transcriptase have been engineered to contain a Glu-Glu-Phe sequence recognized by a monoclonal antibody to alpha-tubulin, YL1/2. Mutated RTs were purified in a single step using peptide elution from columns of immobilized YL1/2. The known sequence requirements of th...

journal_title：FEBS letters

authors： Stammers DK,Tisdale M,Court S,Parmar V,Bradley C,Ross CK

更新日期：1991-06-03 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:Vacuolar membrane H(+)-translocating pyrophosphatase (H(+)-PPase) was purified from pumpkin seedlings. Its enzymatic properties including molecular size of constituting polypeptide (75 kDa) were very similar to those of mung bean H(+)-PPase [(1989) J. Biol. Chem. 264, 20068-20073]. The native, functional molecular siz...

journal_title：FEBS letters

authors： Sato MH,Maeshima M,Ohsumi Y,Yoshida M

更新日期：1991-09-23 00:00:00

abstract：:We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...

journal_title：FEBS letters

authors： Keppler M,Zegrocka O,Strekowski L,Fox KR

更新日期：1999-03-26 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:Stimulation of mast cells through their high affinity IgE receptor (FcεRI) leads to the secretion of pre- and neoformed soluble mediators through vesicular carriers. This process is highly regulated in order to adapt the secretion of these potentially dangerous factors to the physiological needs. This regulation requi...

journal_title：FEBS letters

authors： Benhamou M,Blank U

更新日期：2010-12-15 00:00:00

abstract：:Antigen-induced B cell activation requires mobilization of the Ca(2+) second messenger. This process is associated with the subcellular relocalization of signal effector proteins of the B cell antigen receptor such as the adaptor protein SLP65. Here we describe a broadly applicable live cell imaging method to simultan...

journal_title：FEBS letters

authors： Junek S,Engelke M,Schild D,Wienands J

更新日期：2012-05-21 00:00:00

abstract：:The colony-forming ability of catalase-deficient Escherichia coli mutant genetically modified to produce eicosapentaenoic acid (EPA) showed less decrease than in a control strain producing no EPA, when treated with 0.3mM hydrogen peroxide (H(2)O(2)) under non-growth conditions. H(2)O(2)-induced protein carbonylation w...

journal_title：FEBS letters

authors： Nishida T,Orikasa Y,Watanabe K,Okuyama H

更新日期：2006-12-11 00:00:00

abstract：:Under conditions of molar excess of enzyme, isolated F1-ATPase from beef heart mitochondria catalyses ATP hydrolysis biphasically. The rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. The slow phase of ATP hydrolysis is insensitive to EDTA. [gamma-32P]ATP splitting in th...

journal_title：FEBS letters

authors： Milgrom YaM,Murataliev MB

更新日期：1987-09-28 00:00:00

abstract：:The operation of the human red cell glucose transporter has been studied at normal and high hydrostatic pressure to identify the step(s) which involve a volume change. Pressure inhibited zero-trans and equilibrium exchange influx to similar extents, by decreasing the Vmax but not significantly changing the Km. The Bma...

journal_title：FEBS letters

authors： Thorne SD,Hall AC,Lowe AG

更新日期：1992-04-27 00:00:00

abstract：:We have found that N-acetylneuraminic acid (NANA) consumes toxic hydrogen peroxide (H(2)O(2)) under physiological conditions. Close investigation of this finding revealed that NANA was oxidized by an equimolar amount of H(2)O(2) to provide its decarboxylated product, 4-(acetylamino)-2,4-dideoxy-D-glycero-D-galacto-oct...

journal_title：FEBS letters

authors： Iijima R,Takahashi H,Namme R,Ikegami S,Yamazaki M

更新日期：2004-03-12 00:00:00

abstract：:We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at posi...

journal_title：FEBS letters

authors： Wechselberger C,Severini C,Kreil G,Negri L

更新日期：1998-06-05 00:00:00

abstract：:Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

journal_title：FEBS letters

authors： Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

更新日期：1990-06-04 00:00:00

abstract：:The structure of the dimeric C-terminal domain of the HIV-1 capsid protein (CA), recently determined by X-ray crystallography (Gamble et al. (1997)), has a notable resemblance to the structure of the hepatitis B virus (HBV) capsid protein (Cp) dimer, previously determined by cryo-electron microscopy (Conway et al. (19...

journal_title：FEBS letters

authors： Zlotnick A,Stahl SJ,Wingfield PT,Conway JF,Cheng N,Steven AC

更新日期：1998-07-24 00:00:00

abstract：:To explore the structural basis required for the holoenzyme formation of cAMP-dependent protein kinase, we have prepared rabbit anti-peptide antibodies that can block the holoenzyme formation without affecting the catalytic activity of the enzyme. The antibodies were raised against a specific site in the catalytic (C)...

journal_title：FEBS letters

authors： Sahara S,Sato K,Kaise H,Mori K,Sato A,Aoto M,Tokmakov AA,Fukami Y

更新日期：1996-04-15 00:00:00

abstract：:Uroguanylin, a member of the guanylin peptide family, is a novel peptide regulator for intestinal salt and water transport. A cDNA encoding a precursor for rat uroguanylin was cloned from a rat jejunum cDNA library and sequenced. The precursor was 106 amino acids long and included a 21 residue putative signal peptide ...

journal_title：FEBS letters

authors： Miyazato M,Nakazato M,Matsukura S,Kangawa K,Matsuo H

更新日期：1996-12-02 00:00:00

abstract：:Microinjection of human liver mRNA from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2--10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (alpha 1-antitrypsin PiMM), or water was injected into t...

journal_title：FEBS letters

authors： Bathurst IC,Errington DM,Foreman RC,Judah JD,Carrell RW

更新日期：1985-04-22 00:00:00

abstract：:The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

journal_title：FEBS letters

authors： Wilmotte A,Van der Auwera G,De Wachter R

更新日期：1993-02-08 00:00:00

abstract：:Thymidine kinase in chick embryo retina reaches its highest values on the 8-10th day of development, then declines reaching the lowest value at hatching. The rate of DNA synthesis essentially follows this activity while, in contrast, nucleoside phosphotransferase increases progressively during development. Glucocortic...

journal_title：FEBS letters

authors： Tesoriere G,Tesoriere L,Giuliano M,Vento R

更新日期：1983-11-28 00:00:00