A novel fluorescent marker for assembled mitochondria ATP synthase of yeast. OSCP subunit fused to green fluorescent protein is assembled into the complex in vivo.

Abstract:

:We have shown that OSCP, a subunit of yeast mitochondrial ATP synthase, can be incorporated into the intact enzyme as a fusion protein representing OSCP fused at its C-terminus to the green fluorescent protein (GFP) of Aequorea victoria. The relevant fusion OSCP-GFP-h6 additionally contains a hexahistidine tag at the C-terminus. Expression of OSCP-GFP-h6 in yeast cells lacking endogenous OSCP led to the efficient restoration of growth of cells on the non-fermentable substrate, ethanol. Confocal laser scanning microscopy revealed fluorescence due to GFP in mitochondria of cells expressing OSCP-GFP-h6. Use of immobilised metal ion affinity chromatography enabled the recovery of assembled ATP synthase complexes which contained OSCP-GFP-h6 identified by its mobility on SDS-PAGE and immunoreactivity to anti-OSCP and anti-GFP antibodies. The successful isolation of the assembled multisubunit ATP synthase containing GFP fused to one of the essential subunits of the complex widely expands the potential applications of GFP. In principle, these include the spatial and temporal monitoring of ATP synthase complexes in vivo, and the exploration of interactions involving ATP synthase subunits by fluorescence resonance energy transfer (FRET).

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Prescott M,Lourbakos A,Bateson M,Boyle G,Nagley P,Devenish RJ

doi

10.1016/s0014-5793(97)00670-4

subject

Has Abstract

pub_date

1997-07-07 00:00:00

pages

97-101

issue

1

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(97)00670-4

journal_volume

411

pub_type

杂志文章
  • Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair.

    abstract::Spectral variants of the green fluorescent protein (GFP) have been extensively used as reporters to image molecular interactions in living cells by fluorescence resonance energy transfer (FRET). However, those GFP variants which are the most efficient donor acceptor pairs for FRET measurements show a high degree of sp...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03508-1

    authors: Zimmermann T,Rietdorf J,Girod A,Georget V,Pepperkok R

    更新日期:2002-11-06 00:00:00

  • Lifespan decrease in a Caenorhabditis elegans mutant lacking TRX-1, a thioredoxin expressed in ASJ sensory neurons.

    abstract::Thioredoxins are a class of small proteins that play a key role in regulating many cellular redox processes. We report here the characterization of the first member of the thioredoxin family in metazoans that is mainly associated with neurons. The Caenorhabditis elegans gene B0228.5 encodes a thioredoxin (TRX-1) that ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.12.046

    authors: Miranda-Vizuete A,Fierro González JC,Gahmon G,Burghoorn J,Navas P,Swoboda P

    更新日期:2006-01-23 00:00:00

  • Computational prediction of protein interfaces: A review of data driven methods.

    abstract::Reliably pinpointing which specific amino acid residues form the interface(s) between a protein and its binding partner(s) is critical for understanding the structural and physicochemical determinants of protein recognition and binding affinity, and has wide applications in modeling and validating protein interactions...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2015.10.003

    authors: Xue LC,Dobbs D,Bonvin AM,Honavar V

    更新日期:2015-11-30 00:00:00

  • Peroxiredoxin-I is an autoimmunogenic tumor antigen in non-small cell lung cancer.

    abstract::In eukaryotic cells, peroxiredoxins are both antioxidants and regulators of H(2)O(2)-mediated signaling. We previously found that peroxiredoxin-I (Prx-I) was overexpressed in non-small cell lung cancer (NSCLC) tissue. Since overexpressed protein can induce a humoral immune response, we examined whether serum from NSCL...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.04.028

    authors: Chang JW,Lee SH,Jeong JY,Chae HZ,Kim YC,Park ZY,Yoo YJ

    更新日期:2005-05-23 00:00:00

  • Comparison of the specificities of p70 S6 kinase and MAPKAP kinase-1 identifies a relatively specific substrate for p70 S6 kinase: the N-terminal kinase domain of MAPKAP kinase-1 is essential for peptide phosphorylation.

    abstract::xxR/KxRxxSxx sequences were phosphorylated with high efficiency by both p70 S6 kinase (p70S6K) and MAPKAP kinase-1. The best substrate for MAPKAP kinase-1 (KKKNRTLSVA) was phosphorylated with a Km of 0.17 microM, and the best substrate for p70S6K (KKRNRTLSVA) with a Km of 1.5 microM. The requirement of both enzymes fo...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01170-j

    authors: Leighton IA,Dalby KN,Caudwell FB,Cohen PT,Cohen P

    更新日期:1995-11-20 00:00:00

  • The maximum of the histone acetyltransferase activity precedes DNA-synthesis in regenerating rat liver.

    abstract::A pronounced transitory increase of histone acetyltransferase (EC 2.3.1.48) activity before the onset of DNA replication is shown to occur in regenerating rat liver. This effect is followed by an increase in the H1/H1(0) ratio at the start of DNA synthesis; H1(0) de is replaced by H1. Histone acetylation and H1/H1(0) ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80257-6

    authors: Weiss G,Puschendorf B

    更新日期:1988-09-26 00:00:00

  • The expression of the embryonic gene Cripto-1 is regulated by OCT4 in human embryonal carcinoma NCCIT cells.

    abstract::Cripto-1 and OCT4, expressed in stem cells and cancers, play important roles in tumorigenesis. Here, we demonstrate that Cripto-1 expression is regulated by OCT4 in human embryonic carcinoma NCCIT cells. The endogenous expression of Cripto-1 and OCT4 is significantly reduced during differentiation. Cripto-1 expression...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.12935

    authors: Park SW,Do HJ,Han MH,Choi W,Kim JH

    更新日期:2018-01-01 00:00:00

  • Golgi stress response and organelle zones.

    abstract::Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1002/1873-3468.13554

    authors: Sasaki K,Yoshida H

    更新日期:2019-09-01 00:00:00

  • Rapid purification of wildtype and mutant cytochrome c oxidase from Rhodobacter sphaeroides by Ni(2+)-NTA affinity chromatography.

    abstract::A rapid and highly efficient method of purifying the aa3-type cytochrome c oxidase from Rhodobacter sphaeroides has been developed. This method relies upon a six-histidine affinity tag fused to the C-terminus of subunit I, which confers to the oxidase a high affinity for Ni(2+)-nitrilotriacetic acid (NTA) agarose. The...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)00626-k

    authors: Mitchell DM,Gennis RB

    更新日期:1995-07-10 00:00:00

  • Reanalysis of the involvement of gamma-glutamyl transpeptidase in the cell activation process.

    abstract::The inhibitor of gamma-glutamyl transpeptidase (gamma-GT) acivicin modulates cellular responses including growth, myeloid maturation and apoptosis. Whether these effects result from the inhibition of gamma-GT enzyme activity remains unclear. We compared the cellular effects of acivicin against a more potent and specif...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03057-5

    authors: Antczak C,Karp DR,London RE,Bauvois B

    更新日期:2001-11-16 00:00:00

  • D-Aspartate stimulation of testosterone synthesis in rat Leydig cells.

    abstract::D-Aspartate increases human chorionic gonadotropin-induced testosterone production in purified rat Leydig cells. L-Aspartate, D-,L-glutamate or D-,L-asparagine could not substitute for D-aspartate and this effect was independent of glutamate receptor activation. Testosterone production was enhanced only in cells cultu...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00045-9

    authors: Nagata Y,Homma H,Lee JA,Imai K

    更新日期:1999-02-12 00:00:00

  • Metabolic pathways in Ehrlich ascites tumor cells recovering from a low bioenergetic status.

    abstract::Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightl...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)00759-4

    authors: Dell'Antone P

    更新日期:1994-08-22 00:00:00

  • Evidence of a tissue-restricting DNA regulatory element in the mouse IRBP promoter.

    abstract::The expression of interphotoreceptor retinoid binding protein (IRBP) is limited to photoreceptor cells of the retina and pinealocytes of the pineal gland. We sought to define cis-elements of the mouse IRBP 5' flanking region that are required for this restricted activity. In vitro transient transfections of retinoblas...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02662-x

    authors: Boatright JH,Knox BE,Jones KM,Stodulkova E,Nguyen HT,Padove SA,Borst DE,Nickerson JM

    更新日期:2001-08-24 00:00:00

  • Eicosapentaenoic acid and docosahexaenoic acid are antagonists at the thromboxane A2/prostaglandin H2 receptor in human platelets.

    abstract::The present study investigated the mechanism by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) inhibit platelet activation induced by thromboxane A2. DHA was found to be more potent than EPA in blocking platelet aggregation induced by the stable thromboxane A2 mimetic, U46619. Furthermore, this inhib...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(89)80137-1

    authors: Swann PG,Venton DL,Le Breton GC

    更新日期:1989-01-30 00:00:00

  • Gemin5 plays a role in unassembled-U1 snRNA disposal in SMN-deficient cells.

    abstract::Gemin5 acts as a U1 small nuclear RNA (snRNA)-binding protein in U1 small nuclear ribonucleic protein (snRNP) biogenesis. Here, we report a role for Gemin5 in unassembled U1 snRNP disposal under survival of motor neuron (SMN) protein-deficient conditions. We demonstrate that non-Sm protein-associated U1 snRNA and U1A ...

    journal_title:FEBS letters

    pub_type: 信件

    doi:10.1002/1873-3468.13031

    authors: Jiang D,Zou X,Zhang C,Chen J,Li Z,Wang Y,Deng Z,Wang L,Chen S

    更新日期:2018-04-01 00:00:00

  • A leucine-rich repeat protein of carrot that exhibits antifreeze activity.

    abstract::A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00280-x

    authors: Meyer K,Keil M,Naldrett MJ

    更新日期:1999-03-26 00:00:00

  • In vitro reconstitution of messenger ribonucleoprotein particles from globin messenger RNA and cytosol proteins.

    abstract::Deproteinized globin poly(A) + mRNAs reassociate readily in vitro with soluble RNA-binding proteins of the cytosol; reconstituted messenger ribonucleoprotein complexes are obtained which are very similar to native globin polyribosomal-mRNP as far as bouyant density in Cs2SO4 and the composition of proteins which can b...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(85)80518-4

    authors: Gaedigk R,Oehler S,Köhler K,Setyono B

    更新日期:1985-01-07 00:00:00

  • Molecular cloning in yeast by in vivo homologous recombination of the yeast putative alpha1 subunit of the voltage-gated calcium channel.

    abstract::Saccharomyces cerevisiae has only one gene encoding a putative voltage-gated Ca2+ channel pore-forming subunit, CCH1, which is not possible to be cloned by conventional molecular cloning techniques using Escherichia coli. Here, we report the successful cloning of CCH1 in yeast by in vivo homologous recombination witho...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.09.021

    authors: Iida K,Tada T,Iida H

    更新日期:2004-10-22 00:00:00

  • The effect of local anaesthetics on the ryanodine receptor/Ca2+ release channel of brain microsomal membranes.

    abstract::The effects of various local anaesthetics (LAs) on ryanodine binding of the sheep brain ryanodine receptor were tested. Tetracaine and dibucaine inhibit the binding with half-maximal inhibition (CI50) of 0.12 mM and 0.7 mM, respectively. Lidocaine and its analog QX-314, on the other hand, stimulate the binding up to 3...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)80969-2

    authors: Martin C,Ashley R,Shoshan-Barmatz V

    更新日期:1993-08-09 00:00:00

  • Growth control mechanisms in neuronal regeneration.

    abstract::Neurons grow during development and extend long axons to make contact with their targets with the help of an intrinsic program of axonal growth as well as a range of extrinsic cues and a permissive milieu. Injury events in adulthood induce some neuron types to revert to a regenerative state in the peripheral nervous s...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2015.04.046

    authors: Doron-Mandel E,Fainzilber M,Terenzio M

    更新日期:2015-06-22 00:00:00

  • Binding characteristics of complementary fibronectin fragments on artificial substrata.

    abstract::Various properties have been evaluated for the binding to tissue culture substrata of proteolytic fragments of human plasma or cellular fibronectins containing complementary sequences from the individual and alternatively spliced chains, since related fragments are known to yield differing adhesive responses from cell...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(88)80166-2

    authors: Lewandowska K,Kaetzel CS,Zardi L,Culp LA

    更新日期:1988-09-12 00:00:00

  • Conditional gene modification in mouse liver using hydrodynamic delivery of plasmid DNA encoding Cre recombinase.

    abstract::The success of Cre-mediated conditional gene targeting in liver of mice has until now depended on the generation of Cre recombinase transgenic mice or on viral-mediated transduction. Here, we sought to establish the feasibility of using hydrodynamic gene delivery of Cre recombinase into liver, using a ROSA26 EGFP mous...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2006.06.094

    authors: Zhu HZ,Wang W,Feng DM,Sai Y,Xue JL

    更新日期:2006-08-07 00:00:00

  • The common C-terminal sequences of substance P and neurokinin A contact the same region of the NK-1 receptor.

    abstract::Although neurokinin A (NKA), a tachykinin peptide with sequence homology to substance P (SP), is a weak competitor of radiolabeled SP binding to the NK-1 receptor (NK-1R), more recent direct binding studies using radiolabeled NKA have demonstrated an unexpected high-affinity interaction with this receptor. To document...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)02228-6

    authors: Bremer AA,Leeman SE,Boyd ND

    更新日期:2000-12-01 00:00:00

  • The endoproteolytic processing of alphavbeta5 integrin is involved in cytoskeleton remodelling and cell migration.

    abstract::We previously showed that the post-translational cleavage of alphav subunit is essential for integrin-dependent signalling and cell adhesion. Here, we report that blocking alphav subunit cleavage by expression of alpha1-PDX, a convertase inhibitor, modified the capacity of cells to change shape, via a remodelling of t...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(03)01467-4

    authors: Berthet V,Rigot V,Nejjari M,Marvaldi J,Luis J

    更新日期:2004-01-16 00:00:00

  • The aminoacceptor stem of the yeast tRNA(Lys) contains determinants of mitochondrial import selectivity.

    abstract::Yeast tRNA(Lys)CUU is nucleus-encoded and is partially imported into the mitochondria. Another lysine isoacceptor, tRNA(Lys)SUU, is also nucleus-encoded but is not imported. These two tRNAs differ in 21 bases. We have previously localised import selectivity determinants in the anticodon arm. By in vitro import of muta...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(98)01653-6

    authors: Kazakova HA,Entelis NS,Martin RP,Tarassov IA

    更新日期:1999-01-15 00:00:00

  • Two wavelength-dependent mechanisms of sensitisation of light-induced quenching in the isolated light-harvesting complex II.

    abstract::The efficiency of visible light in inducing fluorescence quenching in the isolated light-harvesting complex II (LHCII) of higher plants is investigated by action spectroscopy in the visible portion of photosynthetic active radiation. The efficiency spectrum displays a relatively homogenous quenching yield across the m...

    journal_title:FEBS letters

    pub_type: 信件

    doi:10.1002/1873-3468.12279

    authors: Santabarbara S,Zubik M,Remelli W,Zucchelli G,Gruszecki WI

    更新日期:2016-08-01 00:00:00

  • Ceramide generated by acidic sphingomyelinase contributes to tumor necrosis factor-alpha-mediated apoptosis in human colon HT-29 cells through glycosphingolipids formation. Possible role of ganglioside GD3.

    abstract::In the present study we assessed the contribution of acidic sphingomyelinase (ASMase), a ceramide generating enzyme, in tumor necrosis factor (TNF)-mediated apoptosis in human colon HT-29 cells. TNF induced apoptosis in HT-29 cells in a time- and dose-dependent fashion. Downregulation of the active endogenous ASMase f...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03140-x

    authors: Colell A,Morales A,Fernández-Checa JC,García-Ruiz C

    更新日期:2002-08-28 00:00:00

  • Amino acid substitution in alpha-helix 7 of Cry1Ac delta-endotoxin of Bacillus thuringiensis leads to enhanced toxicity to Helicoverpa armigera Hubner.

    abstract::Insecticidal proteins or delta-endotoxins of Bacillus thuringiensis are highly toxic to a wide range of agronomically important pests. The toxins are formed of three structural domains. The N-terminal domain is a bundle of eight alpha-helices and is implicated in pore formation in insect midgut epithelial membranes. A...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)01157-6

    authors: Chandra A,Ghosh P,Mandaokar AD,Bera AK,Sharma RP,Das S,Kumar PA

    更新日期:1999-09-17 00:00:00

  • A brilliant monomeric red fluorescent protein to visualize cytoskeleton dynamics in Dictyostelium.

    abstract::Red fluorescent proteins (RFPs) combined with GFP are attractive probes for double-fluorescence labeling of proteins in live cells. However, the application of these proteins is restrained by stable oligomer formation and by their weak fluorescence in vivo. Previous attempts to eliminate these problems by mutagenesis ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2004.09.084

    authors: Fischer M,Haase I,Simmeth E,Gerisch G,Müller-Taubenberger A

    更新日期:2004-11-05 00:00:00

  • Direction of aminoacylated transfer RNAs into antibiotic synthesis and peptidoglycan-mediated antibiotic resistance.

    abstract::Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2013.07.036

    authors: Shepherd J,Ibba M

    更新日期:2013-09-17 00:00:00