Abstract:
:We have isolated a cDNA encoding a precursor of dermorphin from the skin of Pachymedusa dacnicolor. Besides four copies of this opioid peptide, the deduced sequence also contains the genetic information for a novel peptide Tyr-Ile-Phe-His-Leu-Met-Asp-NH2. This differs from Met-deltorphin by the presence of Ile at position 2. In a related precursor from the skin of Agalychnis annae, the sequence of this peptide is in the 3'-untranslated region of the cloned cDNA. From earlier results we predict that in skin peptides the second residue is D-allo-Ile. We have synthesized this and related peptides with different D-amino acids, and determined their delta agonist activity. The peptide with D-nor-Leu binds with high affinity to delta receptors, while that with D-allo-Ile is about 100 times less active.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wechselberger C,Severini C,Kreil G,Negri Ldoi
10.1016/s0014-5793(98)00545-6subject
Has Abstractpub_date
1998-06-05 00:00:00pages
41-3issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(98)00545-6journal_volume
429pub_type
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