Abstract:
:The OxyR protein is a transcriptional activator for a subset of peroxide stress-inducible genes, most of which are involved in defense systems against oxidative stress. Recently, it was demonstrated that purified OxyR has one intramolecular disulfide bond, which led to the proposal that the reversible disulfide bond formation regulates the activity of OxyR as a transcription factor in response to peroxide stress. In this study, I demonstrated by SDS-PAGE under non-reducing conditions that an intramolecular disulfide bond is formed in OxyR upon exposure of the cells to hydrogen peroxide in vivo. Experiments using strains expressing mutant OxyR proteins with Cys to Ser single amino acids substitutions confirmed that the disulfide bond is formed between the Cys-199 and -208. Kinetic analyses indicated that the formation of the disulfide bond is rapid and transient, oxidized within 30 s and re-reduced within 5 min after the addition of hydrogen peroxide in the wild-type strain. These results provide evidence for the regulatory role of the reversible oxidation of dithiol to disulfide in sensing peroxide stress in vivo and signal transduction to the transcription apparatus by OxyR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Tao Kdoi
10.1016/s0014-5793(99)01013-3keywords:
subject
Has Abstractpub_date
1999-08-20 00:00:00pages
90-2issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01013-3journal_volume
457pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Adrenomedullin (ADM) functions as a survival factor against hypoxic cell death. However, molecular mechanisms underlying the cell survival pathway remain largely unknown. In this report, we showed that ADM suppressed reactive oxygen species (ROS) increase by inhibiting reduction of glutathione (GSH) level in hypoxia/r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.063
更新日期:2010-01-04 00:00:00
abstract::Almost all eukaryotic mRNAs acquire a poly(A) tail at the 3'-end by a concerted RNA processing event: cleavage and polyadenylation. The canonical PAP, PAPα, was considered the only nuclear PAP involved in general polyadenylation of mRNAs. A phosphoinositide-modulated nuclear PAP, Star-PAP, was then reported to regulat...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2014.05.029
更新日期:2014-06-27 00:00:00
abstract::As the initiator of DNA double-strand breaks during meiosis in Saccharomyces cerevisiae, the SPO11 protein is essential for recombination. Similarity between SPO11 and archaebacterial TOP6A proteins points to evolutionary specialization of a DNA cleavage function for meiotic recombination. To determine whether this ex...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01546-x
更新日期:1999-12-03 00:00:00
abstract::We analyzed the expression of aquaporins (AQPs) in the small intestine to elucidate their functions, and found that AQP9, which had not previously been detected there, is present in duodenum, jejunum, and ileum. AQP9 is expressed in colon as well, but not in stomach. Also, its expression in these intestinal sections i...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00256-4
更新日期:2003-04-10 00:00:00
abstract::Incubation of rat skeletal muscle mitochondria with the nitric oxide generator, S-nitrosoglutathione (GSNO) reversibly inhibited oxygen utilisation with all substrates tested. The visible absorption spectra of the inhibited mitochondria showed that cytochromes c+c1, b and a+a3 were reduced, indicating a block at the d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00424-2
更新日期:1994-05-23 00:00:00
abstract::PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S pha...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2008.07.017
更新日期:2008-08-20 00:00:00
abstract::The C-Jun N-terminal Kinase (JNK) inhibitor SP600125 is widely used to inhibit the JNK-mediated Bax activation and cell apoptosis. However, this report demonstrates that SP600125 synergistically enhances the dihydroartemisinin (DHA)-induced human lung adenocarcinoma cell apoptosis by accelerating Bax translocation and...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.08.014
更新日期:2010-09-24 00:00:00
abstract::A 26 kDa peptide has been cleaved from the C-terminus of the S1 heavy chain with formic acid. Cleavage occurs in the '50 kDa' domain probably at the Asp-600-Pro-601 bond. This fragment has been renatured in the presence of the A2 light chain and the 26 kDa(A2) complex shown to interact with actin in an ATP-sensitive m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80484-3
更新日期:1989-01-02 00:00:00
abstract::Pseudomonas aeruginosa exotoxin A (ETA) must be proteolytically nicked by furin at Arg279 before being translocated into the cytosol of target cells. A similar cleavage can also be obtained with trypsin. Using this assay we could show that the interaction with lipid bilayers can strongly influence the extent of nickin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01580-9
更新日期:1998-01-16 00:00:00
abstract::Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03059-9
更新日期:2001-11-16 00:00:00
abstract::Human high- and low-Mr kininogens were shown to be potent inhibitors of cysteine proteinases such as cathepsin L and papain (Ki = 17-48 pM). A strong immunological cross-reaction between the kininogens and low-Mr alpha-cysteine proteinase inhibitor from human plasma was found. Comparison of partial amino acid sequence...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80322-7
更新日期:1985-03-25 00:00:00
abstract::The insulin receptor family consists of the homologous tyrosine kinase receptors, insulin receptor (IR), insulin-like growth factor 1 receptor (IGF1R) and insulin receptor-related receptor. The three-dimensional structures of the tyrosine kinase domain of the IR and the first three extracellular domains (L1, Cys-rich ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01509-9
更新日期:1998-12-18 00:00:00
abstract::Tumor necrosis factor receptor associated factor 6 (TRAF6) is an important signaling molecule involved in a diverse array of physiological processes. It has been proposed that TRAF6, a RING finger-containing protein, acts as a ubiquitin ligase (E3) and a target for Lys-63 linked polyubiquitination mediated by Ubc13-Ue...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.038
更新日期:2004-05-21 00:00:00
abstract::Okadaic acid, a specific inhibitor of protein phosphatase 1 in Paramecium causes sustained backward swimming in response to depolarising stimuli (S. Klumpp et al. (1990) EMBO J. 9, 685). Here, we employ okadaic acid, tautomycin, microcystin LR and inhibitor 1 as phosphatase inhibitors to identify a 42 kDa protein in t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81003-q
更新日期:1991-08-19 00:00:00
abstract::A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system with dual amino acid-selective isotope labeling to identify the NMR signal from th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03048-x
更新日期:2002-07-31 00:00:00
abstract::Nucleoside modifications in tRNA anticodons regulate ribosome dynamics during translation elongation and, thereby, fine-tune global protein synthesis rates. The highly conserved eukaryotic Elongator complex conducts specific C5-substitutions in tRNA wobble base uridines. It harbors two copies of each of its six indivi...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.12865
更新日期:2018-02-01 00:00:00
abstract::As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12143
更新日期:2016-04-01 00:00:00
abstract::We show that some arbitrarily chosen DNA sequences have the ability to influence the positioning of RecA monomers in RecA-DNA complexes. The preferential phase of binding of RecA monomers is shown to depend on the DNA sequence and its nucleotide composition. A simple rearrangement of bases in a limited DNA stretch inf...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00572-6
更新日期:2003-07-10 00:00:00
abstract::To obtain information on the biological significance of yeast fructose-2,6-bisphosphate 6-phosphatase, kinetic data of the purified enzyme [(1987) Eur. J. Biochem. 164, 27-30] have been measured. Maximal activity was found between pH 6 and 7, the apparent Michaelis constant with fructose 2,6-bisphosphate was 7.2 micro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80547-7
更新日期:1988-10-24 00:00:00
abstract::The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80795-6
更新日期:1988-02-29 00:00:00
abstract::The Ca(2+)-calmodulin dependent protein phosphatase, calcineurin, is thought to mediate the action of the two immunosuppressants, cyclosporin A (CsA) and FK506. Calcineurin from all species consists of a catalytic A subunit and a regulatory peptide B, which plays an essential role in catalysis. The enzymatic function ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01365-8
更新日期:1995-01-03 00:00:00
abstract::Cyclic AMP (cAMP) and its action by way of cAMP-dependent protein kinase is important for sperm motility. Previous studies on germ cells have demonstrated a selective decrease in the amount of type I cAMP-dependent protein kinase during spermatid development, and that type II was the major form present in elongating s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81163-3
更新日期:1988-03-14 00:00:00
abstract::H/D isotope exchange effects on P680+. reduction by Yz and electron abstraction from the water oxidizing complex (WOC) in redox state S3 by YZOX were analyzed in PS II core complexes from spinach by measurements of laser flash induced absorption changes at 820 nm and 355 nm. The results obtained reveal: (1) the rate o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01433-0
更新日期:1996-01-08 00:00:00
abstract::Posttranslational modifications of circadian oscillator components are crucial for the generation of circadian rhythms. Among those phosphorylation plays key roles ranging from regulating degradation, complex formation, subcellular localization and activity. Although most of the known clock proteins are phosphoprotein...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.02.038
更新日期:2011-05-20 00:00:00
abstract::We analysed the biochemical properties of insulin receptors of a Type A insulin resistant patient with a single heterozygous point mutation substituting Gln for Arg1174. Insulin binding capacity and affinity to Epstein-Barr virus transformed lymphocytes was normal. Quantitative analysis of autophosphorylation and subs...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00876-0
更新日期:1994-09-05 00:00:00
abstract::The primary structure of a new sarafotoxin, SRTX-d, from the venom of Atractaspis engaddensis is described. SRTX-d differs from SRTX-b in two substitutions: Ile19 instead of Val and Thr2 instead of Ser. The toxicity of SRTX-d and its vasoconstriction potency are very low in comparison to SRTX-a and SRTX-b, whereas its...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81706-5
更新日期:1989-10-09 00:00:00
abstract::Schizosaccharomyces pombe cells take up D-gluconate, as an alternative carbon source for growth, during glucose starvation or when cultured on glycerol-containing medium. Gluconate uptake is not detectable while cells are growing logarithmically on glucose. The addition of D-glucose as well as its non-metabolizable an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)01052-6
更新日期:1996-10-21 00:00:00
abstract::Several mRNAs are known to be targeted to dendrites in hippocampal neurons. In this study, we show that brain-derived neurotrophic factor (BDNF) mRNA has two distinct cis-acting dendritic targeting elements in the short 3' untranslated region (UTR): a constitutive element and an activity-dependent one. Moreover, delet...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.040
更新日期:2010-08-04 00:00:00
abstract::MeCP2 is a chromatin-associated protein that is mutated in Rett syndrome. Its methyl-CpG-binding domain interacts with DNA containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use in vitro and in vivo assays to investigate the DNA binding specificity of two AT-...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12328
更新日期:2016-09-01 00:00:00
abstract::Rat liver plasma membrane removed sialic acid from mixed bovine brain gangliosides more efficiently than from sialyllactose and orosomucoid with an optimal pH of 4.5. When individual gangliosides, each labeled with [14C]sialic acid or [3H]sphingosine, were tested, not only GD1a and GM3 but also GM2 and GM1, both of wh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80985-1
更新日期:1986-10-06 00:00:00