Glucosyl epi-cyclophellitol allows mechanism-based inactivation and structural analysis of human pancreatic α-amylase.

abstract：:cDNA clones encoding the 45 kDa protein were isolated from a rat olfactory epithelium cDNA library and their inserts were sequenced. The reconstructed protein sequence comprises 400 amino acids with a calculated molecular mass of 46,026 Da. A homology was revealed between the amino acid sequence of the 45 kDa protein ...

journal_title：FEBS letters

authors： Merkulova MI,Andreeva SG,Shuvaeva TM,Novoselov SV,Peshenko IV,Bystrova MF,Novoselov VI,Fesenko EE,Lipkin VM

更新日期：1999-04-30 00:00:00

abstract：:The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this ...

journal_title：FEBS letters

authors： Ledent P,Duez C,Vanhove M,Lejeune A,Fonzé E,Charlier P,Rhazi-Filali F,Thamm I,Guillaume G,Samyn B,Devreese B,Van Beeumen J,Lamotte-Brasseur J,Frère JM

更新日期：1997-08-18 00:00:00

abstract：:Connivance of cellular factors during virus-host cell membrane fusion is poorly understood. We have recently shown that cellular villin plays an important role during membrane fusion of reconstituted Sendai virosomes with hepatocytes. Here, we employed villin-null Chinese Hamster Ovary (CHO) cells, where villin expres...

journal_title：FEBS letters

authors： Chandra S,Kumar M,Sharma NR,Sarkar DP

更新日期：2019-08-01 00:00:00

abstract：:In eukaryotes, members of the Ero1 family control oxidative protein folding in the endoplasmic reticulum (ER). Yeast Ero1p is tightly associated with the ER membrane, despite cleavage of the leader peptide, the only hydrophobic sequence that could mediate lipid insertion. In contrast, human Ero1-Lalpha and a yeast mut...

journal_title：FEBS letters

authors： Pagani M,Pilati S,Bertoli G,Valsasina B,Sitia R

更新日期：2001-11-09 00:00:00

abstract：:Two fatty acid ethyl ester (FAEE) synthase isoenzymes purified from human myocardium were reported to be glutathione S-transferases (GST) [(1989) Proc. Natl. Acad. Sci. USA 86, 4470-4473; and (1989) J. Clin. Invest. 84, 1942-1946]. In the present study, the FAEE synthase activity of several purified and well character...

journal_title：FEBS letters

authors： Suzuki T,Kovacs MS,Board PG

更新日期：1990-11-26 00:00:00

abstract：:The 5 alpha-reductase activities in human prostatic nuclei and microsomes were compared. The activities in both subcellular fractions were identical with respect to pH dependence, heat inactivation and Michaelis constants for NADPH and testosterone. Subcellular distribution studies using DNA and nicotinamide mononucle...

journal_title：FEBS letters

authors： Houston B,Chisholm GD,Habib FK

更新日期：1985-06-17 00:00:00

abstract：:The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD...

journal_title：FEBS letters

authors： Collados R,Andreu V,Picorel R,Alfonso M

更新日期：2006-09-04 00:00:00

abstract：:Furin, a member of the subtilisin-like pro-protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of th...

journal_title：FEBS letters

authors： Denault J,Bissonnette L,Longpré J,Charest G,Lavigne P,Leduc R

更新日期：2002-09-11 00:00:00

abstract：:Enhancement by thrombin of Ca2+-dependent 5HT secretion in the absence of added GTP decreases as the time between electropermeabilisation and addition of thrombin is increased. No decrease occurs if thrombin is added with GTP. Observation of apparent GTP-independent receptor/phospholipase C coupling may result from th...

journal_title：FEBS letters

authors： Knight DE,Scrutton MC

更新日期：1987-10-19 00:00:00

abstract：:The [Cu(I)-Cu(II)] half-met form of the dinuclear copper site of tyrosinase has been probed by continuous wave electron paramagnetic resonance (EPR) and hyperfine sublevel correlation (HYSCORE) spectroscopy in the presence and absence of inhibitors. In all cases the EPR spectrum is indicative of a d(x(2)-y(2)) ground ...

journal_title：FEBS letters

authors： van Gastel M,Bubacco L,Groenen EJ,Vijgenboom E,Canters GW

更新日期：2000-06-02 00:00:00

abstract：:A chemokine receptor, CXCR4, and its endogenous ligand, stromal cell-derived factor-1 (SDF-1), have been recognized to be involved in the metastasis of several types of cancers. T140 analogs are peptidic CXCR4 antagonists composed of 14 amino acid residues that were previously developed as anti-HIV agents having inhib...

journal_title：FEBS letters

authors： Tamamura H,Hori A,Kanzaki N,Hiramatsu K,Mizumoto M,Nakashima H,Yamamoto N,Otaka A,Fujii N

更新日期：2003-08-28 00:00:00

abstract：:13CO NMR chemical shifts and 12CO infrared stretching frequencies have been measured for cytochrome P-450cam-CO in the presence of D-camphor and of various camphor analogues. A linear correlation between both parameters delta (13C) and v(CO) was found indicating that the steric and electrostatic interactions acting on...

journal_title：FEBS letters

authors： Legrand N,Bondon A,Simonneaux G,Jung C,Gill E

更新日期：1995-05-08 00:00:00

abstract：:Biological systems, from macromolecules to whole organisms, are robust if they continue to function, survive, or reproduce when faced with mutations, environmental change, and internal noise. I focus here on biological systems that are robust to mutations and ask whether such systems are more or less evolvable, in the...

journal_title：FEBS letters

authors： Wagner A

更新日期：2005-03-21 00:00:00

abstract：:A low resolution solution structure of the IIA domain of the Bacillus subtilis phosphoenolpyruvate-sugar phosphotransferase system (PTS) glucose permease has been determined using 945 inter-residue and 724 intra-residue distance constraints derived from three-dimensional 15N and 13C edited NOESY spectra. A total of 15...

journal_title：FEBS letters

authors： Fairbrother WJ,Gippert GP,Reizer J,Saier MH Jr,Wright PE

更新日期：1992-01-20 00:00:00

abstract：:Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...

journal_title：FEBS letters

authors： Huang J,Xu LG,Liu T,Zhai Z,Shu HB

更新日期：2006-02-06 00:00:00

abstract：:Formylmethanofuran dehydrogenase, a key enzyme of methanogenesis, was purified 100-fold from methanol grown Methanosarcina barkeri to apparent homogeneity and a specific activity of 34 mumol.min-1.mg protein-1. Molybdenum was found to co-migrate with the enzyme activity. The molybdenum content of purified preparations...

journal_title：FEBS letters

authors： Karrasch M,Börner G,Enssle M,Thauer RK

更新日期：1989-08-14 00:00:00

abstract：:Electron paramagnetic resonance (EPR) spectroscopy and analysis of the primary structure of the CMP-N-acetylneuraminic acid hydroxylase revealed that this enzyme is the first iron-sulphur protein of the Rieske type to be found in the cytosol of Eukarya. The dithionite-reduced hydroxylase exhibited an EPR signal known ...

journal_title：FEBS letters

authors： Schlenzka W,Shaw L,Kelm S,Schmidt CL,Bill E,Trautwein AX,Lottspeich F,Schauer R

更新日期：1996-05-06 00:00:00

abstract：:Titin is a giant protein which extends from Z-line to M-line in striated muscles. We report here the purification of a 150-kDa titin fragment, obtained after V8 protease treatment of myofibrils. This polypeptide was located at the N1-line level, in a titin part known to exhibit stiff properties correlated to an associ...

journal_title：FEBS letters

authors： Astier C,Raynaud F,Lebart MC,Roustan C,Benyamin Y

更新日期：1998-06-05 00:00:00

abstract：:Mitochondria of amoeba Acanthamoeba castellanii, a non-photosynthetic soil amoeboid protozoon, possess an uncoupling protein (AcUCP) that mediates free fatty acid-activated proton re-uptake dissipating the proton electrochemical gradient built up by respiration. The present study provides the first evidence that UCP c...

journal_title：FEBS letters

authors： Jarmuszkiewicz W,Antos N,Swida A,Czarna M,Sluse FE

更新日期：2004-07-02 00:00:00

abstract：:Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

journal_title：FEBS letters

authors： Fong JT,Nimlamool W,Falk MM

更新日期：2014-03-03 00:00:00

abstract：:PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...

journal_title：FEBS letters

authors： Wang Z,Takemori H,Halder SK,Nonaka Y,Okamoto M

更新日期：1999-06-18 00:00:00

abstract：:Sterically hindered cyclic amines react with hydroxyl radicals in the presence of oxygen to yield stable nitroxide radicals which can be detected by EPR. This reaction provides a nonconventional spin-trapping tool for detection of hydroxyl radicals. ...

journal_title：FEBS letters

authors： Rosenthal I,Krishna CM,Yang GC,Kondo T,Riesz P

更新日期：1987-09-28 00:00:00

abstract：:Rho-associated kinase 2 (Rock2) is known to promote tumorigenesis in hepatocellular carcinoma (HCC). CCAAT/enhancer-binding protein delta (CEBPD) functions as a tumor suppressor. In this study, we found that the expression of Rock2 and CEBPD are inversely correlated. Knockdown of Rock2 increased CEBPD expression and i...

journal_title：FEBS letters

authors： Li M,Zhou W,Yuan R,Chen L,Liu T,Huang D,Hao L,Xie Y,Shao J

更新日期：2015-04-13 00:00:00

abstract：:The activation of peroxisome proliferator-activated receptor gamma (PPARgamma) has been shown to inhibit the production and the effects of proinflammatory cytokines. Since interleukin-1beta (IL-1beta) directly mediates cartilage degradation in osteoarthritis, we investigated the capability of PPARgamma ligands to modu...

journal_title：FEBS letters

authors： Boyault S,Simonin MA,Bianchi A,Compe E,Liagre B,Mainard D,Bécuwe P,Dauça M,Netter P,Terlain B,Bordji K

更新日期：2001-07-13 00:00:00

abstract：:Transmembrane helices (TMHs) in membrane proteins can be orientated with their N-terminus towards the cytoplasm (Nin), or facing the non-cytoplasmic side (Nout). Most membrane proteins are inserted co-translationally into membranes, aided by Sec-type translocons. Since the final orientation of Nin- and Nout-orientated...

journal_title：FEBS letters

authors： Cymer F,Ismail N,von Heijne G

更新日期：2014-05-21 00:00:00

abstract：:CXCR2 plays a key role in protecting the integrity of the endothelium. Emerging evidence has demonstrated that the long ncRNAs (lncRNA) Human metastasis associated lung adenocarcinoma transcript 1 (MALAT1) participates in the regulation of the pathophysiological processes. However, whether there is crosstalk between C...

journal_title：FEBS letters

authors： Tang Y,Jin X,Xiang Y,Chen Y,Shen CX,Zhang YC,Li YG

更新日期：2015-10-07 00:00:00

abstract：:The requirement of divalent cations for binding of the 46 kDa mannose 6-phosphate receptors to phosphomannan and pentamannose 6-phosphate-substituted bovine serum albumin was examined. Receptors from human liver and human brain bound to both affinity ligands in the absence or presence of divalent cations with similar ...

journal_title：FEBS letters

authors： Junghans U,Waheed A,von Figura K

更新日期：1988-09-12 00:00:00

abstract：:The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solu...

journal_title：FEBS letters

authors： Benie AJ,Whitford D,Hargittai B,Barany G,Janes RW

更新日期：2000-07-07 00:00:00

abstract：:We investigated here whether adiponectin can exhibit an inhibitory effect on tumor necrosis factor-alpha (TNF-alpha)- and receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis by using RAW264 cell D clone with a high efficiency to form osteoclasts. Globular adiponectin (gAd) strongly in...

journal_title：FEBS letters

authors： Yamaguchi N,Kukita T,Li YJ,Kamio N,Fukumoto S,Nonaka K,Ninomiya Y,Hanazawa S,Yamashita Y

更新日期：2008-02-06 00:00:00

abstract：:Cytochrome bd is a bacterial respiratory oxidase carrying three hemes but no copper. We show that nitric oxide (NO) reacts with the intermediate F of cytochrome bd from Azotobacter vinelandii: (i) with a 1:1 stoichiometry, (ii) rapidly (k=1.2 +/- 0.1 x 10(5)M(-1)s(-1) at 20 degrees C), and (iii) yielding the oxidized ...

journal_title：FEBS letters

authors： Borisov VB,Forte E,Sarti P,Brunori M,Konstantinov AA,Giuffrè A

更新日期：2006-09-04 00:00:00