Abstract:
:The 5 alpha-reductase activities in human prostatic nuclei and microsomes were compared. The activities in both subcellular fractions were identical with respect to pH dependence, heat inactivation and Michaelis constants for NADPH and testosterone. Subcellular distribution studies using DNA and nicotinamide mononucleotide adenyl transferase as nuclear markers showed that the amount of 5 alpha-reductase present in the microsomes was directly proportional to the amount of nuclear contamination. These results indicate that human prostatic tissue contains only one form of 5 alpha-reductase, which is located exclusively in the nucleus. This finding has important implications for the mechanism of steroid action in the prostate.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Houston B,Chisholm GD,Habib FKdoi
10.1016/0014-5793(85)80912-1subject
Has Abstractpub_date
1985-06-17 00:00:00pages
231-5issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)80912-1journal_volume
185pub_type
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