Abstract:
:We have used the polymerase chain reaction (PCR) to synthesise a cDNA encoding part of human cardiac troponin I. Amplification was achieved using fully degenerate sets of oligonucleotides corresponding to conserved regions of amino acid sequence identified in other troponin I isoforms. The cloned PCR fragment was subsequently used to isolate full-length cDNAs from a cardiac cDNA library. We describe the approach, as a general cloning strategy starting from limited amino-acid sequence data and report the cloning, and complete amino acid sequence of human cardiac troponin I. Analysis of human development using these clones demonstrates early expression of this gene in the heart.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Vallins WJ,Brand NJ,Dabhade N,Butler-Browne G,Yacoub MH,Barton PJdoi
10.1016/0014-5793(90)81234-fsubject
Has Abstractpub_date
1990-09-17 00:00:00pages
57-61issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81234-Fjournal_volume
270pub_type
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