Primary and post-irradiation inactivation of the sulfhydryl enzyme malate synthase: correlation of protective effects of additives.


:The presence of additives during X-irradiation of malate synthase led to radioprotective effects against primary and post-irradiation inactivation. Pronounced effects were provided by typical scavengers, sulfhydryl reagents and specific ligands (substrates, products, analogues). The results show that scavenging and specific protection are responsible for the protective efficiency of additives. Scavengers delete noxious species formed during irradiation or post-radiationem. Sulfhydryl reagents may act as repair substances. Specific ligands protect the active site of the enzyme and the essential sulfhydryls; specific protection is more pronounced post-radiationem. Ligands and sulfhydryl reagents may additionally act as scavengers. A cumulative index for the protective power of additives against both sorts of inactivation was established.




FEBS letters


Durchschlag H,Zipper P




Has Abstract


1988-09-12 00:00:00














  • Binding of Ala-scanning analogs of omega-conotoxin MVIIC to N- and P/Q-type calcium channels.

    abstract::omega-Conotoxin MVIIC binds to P/Q-type calcium channels with high affinity and N-type channels with low affinity. To reveal the residues essential for subtype selectivity, we synthesized Ala-scanning analogs of MVIIC. Binding assays using rat cerebellar P(2) membranes suggested that Thr(11), Tyr(13) and Lys(2) are es...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sato K,Raymond C,Martin-Moutot N,Sasaki T,Ohtake A,Minami K,Van Renterghem C,Kim JI,Takahashi M,Seagar MJ

    更新日期:2000-03-10 00:00:00

  • Biochemical characterization of glutaredoxins from Chlamydomonas reinhardtii: kinetics and specificity in deglutathionylation reactions.

    abstract::Protein deglutathionylation is mainly catalyzed by glutaredoxins (GRXs). We have analyzed the biochemical properties of four of the six different GRXs of Chlamydomonas reinhardtii. Kinetic parameters were determined for disulfide and dehydroascorbate reduction but also for deglutathionylation of artificial and protein...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Gao XH,Zaffagnini M,Bedhomme M,Michelet L,Cassier-Chauvat C,Decottignies P,Lemaire SD

    更新日期:2010-06-03 00:00:00

  • Structure of the 16 S ribosomal RNA of the thermophilic cyanobacterium Chlorogloeopsis HTF ('Mastigocladus laminosus HTF') strain PCC7518, and phylogenetic analysis.

    abstract::The thermophilic cyanobacterial strain, PCC7518, originally identified as 'Mastigocladus laminosus HTF' does not show branchings or heterocysts. The absence of branchings supports the later assignment to the genus Chlorogloeopsis. The absence of heterocysts may be the result of a mutation because heterocysts were obse...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Wilmotte A,Van der Auwera G,De Wachter R

    更新日期:1993-02-08 00:00:00

  • Tomosyn guides SNARE complex formation in coordination with Munc18 and Munc13.

    abstract::As a SNARE binding protein, tomosyn has been reported to negatively regulate synaptic exocytosis via arresting syntaxin-1 and SNAP-25 into a nonfusogenic product that precludes synaptobrevin-2 entry, raising the question how the assembly of the SNARE complex is achieved. Here, we have investigated new functions of tom...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Li Y,Wang S,Li T,Zhu L,Ma C

    更新日期:2018-04-01 00:00:00

  • Identification of a novel Bcl-xL phosphorylation site regulating the sensitivity of taxol- or 2-methoxyestradiol-induced apoptosis.

    abstract::Bcl-xL, a close homolog of Bcl2, is an important regulator of apoptosis and is overexpressed in human cancer. Phosphorylation of Bcl-xL can be induced by microtubule-damaging drugs such as taxol or 2-methoxyestradiol (2-ME). By site-directed mutagenesis studies, we have identified that serine 62 is the necessary site ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Basu A,Haldar S

    更新日期:2003-03-13 00:00:00

  • Effect of drugs, peptide hormones and lipogenic precursors on the relative incorporation of [3H]H2O and carbon into hepatic cholesterol.

    abstract::Measurement of the weight of desmosterol produced during its biosynthesis in the presence of tritiated water and triparanol has permitted a direct determination of the relative flux of carbon and tritium (the H/C ratio) into sterol in hepatocytes. The H/C ratio increased with time of incubation irrespective of the nut...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Björnsson OG,Pullinger CR,Gibbons GF

    更新日期:1985-08-05 00:00:00

  • Conformation of colicin A: apparent difference between cytoplasmic and extracellular polypeptide chain.

    abstract::Cytoplasmic colicin A has the ability to bind to membranes and to form stable dimers. This form remains stable even in the presence of 1% SDS at 25 degrees C. Both of these properties were not observed for extracellular colicin A suggesting a possible difference in the conformation between cytoplasmic and extracellula...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Knibiehler M,Lazdunski C

    更新日期:1987-06-01 00:00:00

  • Exploration of the spontaneous fluctuating activity of single enzyme molecules.

    abstract::Single enzyme molecules display inevitable, stochastic fluctuations in their catalytic activity. In metabolism, for instance, the stochastic activity of individual enzymes is averaged out due to their high copy numbers per single cell. However, many processes inside cells rely on single enzyme activity, such as transc...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schwabe A,Maarleveld TR,Bruggeman FJ

    更新日期:2013-09-02 00:00:00

  • The surfactant lipid transporter ABCA3 is N-terminally cleaved inside LAMP3-positive vesicles.

    abstract::ABCA3 mutations cause fatal surfactant deficiency and interstitial lung disease. ABCA3 protein is a lipid transporter indispensible for surfactant biogenesis and storage in lamellar bodies (LB). The protein folds in endoplasmic reticulum and is glycosylated in Golgi en route to the membrane of mature LB and their prec...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Engelbrecht S,Kaltenborn E,Griese M,Kern S

    更新日期:2010-10-22 00:00:00

  • p21-activated protein kinase gamma-PAK in pituitary secretory granules phosphorylates prolactin.

    abstract::p21-activated protein kinase gamma-PAK phosphorylates prolactin (PRL) in rat pituitary secretory granules on Ser-177 and on the equivalent site, Ser-179, in recombinant human PRL. This is shown by comparison of phosphopeptide maps with the human PRL mutant S179D. gamma-PAK is present in rat and bovine granules as iden...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tuazon PT,Lorenson MY,Walker AM,Traugh JA

    更新日期:2002-03-27 00:00:00

  • Inhibition by amiloride and by Na+-depletion of A23187-stimulated arachidonic acid and histamine release from rat mast cells.

    abstract::Rat peritoneal mast cells, labelled with [14C]arachidonic acid, released histamine and [14C]arachidonic acid upon the addition of A23187. The release induced by low concentrations of A23187 was suppressed by removal of extracellular Na+ and by addition of the Na+/H+ exchange inhibitor, amiloride. Addition of protein k...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Linnebjerg H,Hansen HS,Jensen B

    更新日期:1988-11-21 00:00:00

  • Adaptive evolution in the snake venom Kunitz/BPTI protein family.

    abstract::Snake venoms are rich sources of serine proteinase inhibitors that are members of the Kunitz/BPTI (bovine pancreatic trypsin inhibitor) family. However, only a few of their gene sequences have been determined from snakes. We therefore cloned the cDNAs for the trypsin and chymotrypsin inhibitors from a Vipera ammodytes...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Zupunski V,Kordis D,Gubensek F

    更新日期:2003-07-17 00:00:00

  • Identification of creatine kinase isoenzymes in the guinea-pig. Presence of mitochondrial creatine kinase in smooth muscle.

    abstract::Isoenzymes of creatine kinase (CK, EC in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ishida Y,Wyss M,Hemmer W,Wallimann T

    更新日期:1991-05-20 00:00:00

  • Regulation of the F0F1-ATP synthase: the conformation of subunit epsilon might be determined by directionality of subunit gamma rotation.

    abstract::F(0)F(1)-ATP synthase couples ATP synthesis/hydrolysis with transmembrane proton transport. The catalytic mechanism involves rotation of the gamma epsilon c(approximately 10)-subunits complex relative to the rest of the enzyme. In the absence of protonmotive force the enzyme is inactivated by the tight binding of MgAD...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Feniouk BA,Junge W

    更新日期:2005-09-26 00:00:00

  • Human Z alpha 1-antitrypsin accumulates intracellularly and stimulates lysosomal activity when synthesised in the Xenopus oocyte.

    abstract::Microinjection of human liver mRNA from a patient homozygous for alpha 1-antitrypsin deficiency (PiZZ) into Xenopus oocytes led to a 2--10-fold increase in lysosomal activity. Stimulation of lysosomal activity was not observed when mRNA from a normal human liver (alpha 1-antitrypsin PiMM), or water was injected into t...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bathurst IC,Errington DM,Foreman RC,Judah JD,Carrell RW

    更新日期:1985-04-22 00:00:00

  • Two sites of primary degradation of the D1-protein induced by acceptor or donor side photo-inhibition in photosystem II core complexes.

    abstract::Depending on experimental conditions we have found that photo-inhibitory treatment of photosystem II (PSII) core complexes, isolated from wheat, can generate two fragments of about 23-24 kDa that contain either the C-terminal or N-terminal regions of the D1-protein. A 24 kDa C-terminal fragment appears when the water ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: De Las Rivas J,Andersson B,Barber J

    更新日期:1992-04-27 00:00:00

  • Inhibition by somatostatin of the vasopressin-stimulated adenylate cyclase in a kidney-derived line of cells grown in defined medium.

    abstract::LLC-PK1L cells, a kidney-derived cell line grown in defined medium, possess a vasopressin-sensitive adenylate cyclase. Somatostatin was able to inhibit the vasopressin-induced increase in adenylate cyclase activity, without affecting the basal enzyme activity. This inhibition was competitive. No effect of somatostatin...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Roy C

    更新日期:1984-04-24 00:00:00

  • Curcumin mediated apoptosis in AK-5 tumor cells involves the production of reactive oxygen intermediates.

    abstract::Curcumin, the active ingredient of the rhizome of Curcuma longa has anti-inflammatory, antioxidant and antiproliferative activities. Although its precise mode of action remains elusive, studies have shown that chemopreventive action of curcumin might be due to its ability to induce apoptosis in cancer cells. Curcumin ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bhaumik S,Anjum R,Rangaraj N,Pardhasaradhi BV,Khar A

    更新日期:1999-08-06 00:00:00

  • Essential structure for full enterotoxigenic activity of heat-stable enterotoxin produced by enterotoxigenic Escherichia coli.

    abstract::Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoshimura S,Ikemura H,Watanabe H,Aimoto S,Shimonishi Y,Hara S,Takeda T,Miwatani T,Takeda Y

    更新日期:1985-02-11 00:00:00

  • Identification of the first Rho-GEF inhibitor, TRIPalpha, which targets the RhoA-specific GEF domain of Trio.

    abstract::The Rho-guanine nucleotide exchange factors (Rho-GEFs) remodel the actin cytoskeleton via their Rho-GTPase targets and affect numerous physiological processes such as transformation and cell motility. They are therefore attractive targets to design specific inhibitors that may have therapeutic applications. Trio conta...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Schmidt S,Diriong S,Méry J,Fabbrizio E,Debant A

    更新日期:2002-07-17 00:00:00

  • Inhibition and enhancement of odorant-induced cAMP accumulation in rat olfactory cilia by antibodies directed against G alpha S/olf- and G alpha i-protein subunits.

    abstract::The odorant-induced accumulation of cAMP can be inhibited by antibodies directed against G alpha s/olf. In contrast, antibodies raised against G alpha i-subunits caused a strong enhancement of the odorant-induced cAMP accumulation. Western blotting and immunoelectron microscopy revealed the presence of both G alpha s/...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Sinnarajah S,Ezeh PI,Pathirana S,Moss AG,Morrison EE,Vodyanoy V

    更新日期:1998-04-24 00:00:00

  • Adipogenesis inhibitory factor. A novel inhibitory regulator of adipose conversion in bone marrow.

    abstract::Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature for...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohsumi J,Miyadai K,Kawashima I,Ishikawa-Ohsumi H,Sakakibara S,Mita-Honjo K,Takiguchi Y

    更新日期:1991-08-19 00:00:00

  • Assembly of paired helical filaments from mouse tau: implications for the neurofibrillary pathology in transgenic mouse models for Alzheimer's disease.

    abstract::In Alzheimer's disease and related dementias, human tau protein aggregates into paired helical filaments and neurofibrillary tangles. However, such tau aggregates have not yet been demonstrated in transgenic mouse models of the disease. One of the possible explanations would be that mouse tau has different properties ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kampers T,Pangalos M,Geerts H,Wiech H,Mandelkow E

    更新日期:1999-05-14 00:00:00

  • Two excited states in aequorin bioluminescence induced by tryptophan modification.

    abstract::The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohmiya Y,Ohashi M,Tsuji FI

    更新日期:1992-04-20 00:00:00

  • Transcriptional regulation of murine NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase.

    abstract::The cytosolic NADP(+)-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase is ubiquitously expressed in all mouse tissues and cell lines examined. Northern analyses of the RNA indicated that there is an extensive variation in the levels of mRNA in ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Peri KG,MacKenzie RE

    更新日期:1991-12-02 00:00:00

  • Electrons generated by photosystem II are utilized by an oxidase in the absence of photosystem I in the cyanobacterium Synechocystis sp. PCC 6803.

    abstract::The reduction and reoxidation kinetics of the first quinone-type electron acceptor in photosystem II, QA-, were measured by fluorescence in a light-tolerant, photosystem I-less strain of the cyanobacterium Synechocystis sp. PCC 6803. In this strain, which shows excellent amplitudes of variable fluorescence, the rate o...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Vermaas WF,Shen G,Styring S

    更新日期:1994-01-03 00:00:00

  • Glycine and other amino compounds prevent chromatin precipitation at physiological ionic strength.

    abstract::Glycine, proline and taurine, when present in the range 0.1-0.60 M, inhibit chromatin precipitation by sodium chloride. Histone gel electrophoresis revealed that the linker histones H1 and H5 were largely depleted from the supernatant chromatin fraction at 0.2 M NaCl, while this depletion was absent in the presence of...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Buche A,Ouassaidi A,Hacha R,Delpire E,Gilles R,Houssier C

    更新日期:1989-04-24 00:00:00

  • Functional disruption of IEX-1 expression by concatemeric hammerhead ribozymes alters growth properties of 293 cells.

    abstract::The early response gene IEX-1 modulates apoptosis and cell growth in a poorly defined fashion. Here, we describe the effect of hammerhead ribozymes specifically disrupting IEX-1 expression in 293 cells. Compared to vector control, 293 cells exhibit a reduced growth rate and a slowed cell cycle progression, when stably...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Grobe O,Arlt A,Ungefroren H,Krupp G,Fölsch UR,Schmidt WE,Schäfer H

    更新日期:2001-04-13 00:00:00

  • Interactome modeling.

    abstract::A long-term goal of the field of interactome modeling is to understand how global and local properties of complex macromolecular networks impact on observable biological properties, and how changes in such properties can lead to human diseases. The information available at this stage of development of the field provid...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Vidal M

    更新日期:2005-03-21 00:00:00

  • NMR measurement of the off rate from the first calcium-binding site of the synaptotagmin I C2A domain.

    abstract::The off rate from the first calcium-binding site of the C2A domain of synaptotagmin I, a putative calcium receptor in neurotransmitter release, has been determined by 15N-nuclear magnetic resonance relaxation dispersion measurements. The exchange rate was obtained by fitting the dependence of the transverse relaxation...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Millet O,Bernadó P,Garcia J,Rizo J,Pons M

    更新日期:2002-04-10 00:00:00