Abstract:
:Protease involvement has been implicated in the signalling process of activation-induced apoptosis. Here we report the isolation of a protease from Jurkat T cells undergoing Fas-induced apoptosis. Although the protease probably is a serine protease, it seems to be distantly related to members of the ICE/ced-3/Ich-1(nedd-2) family. In a cell-free system using isolated thymocyte nuclei, the protease rapidly induces DNA fragmentation and morphological changes typically seen in apoptosis. Our results clearly show protease activation downstream to Fas-ligation and implicate an important role for the isolated protease in signalling of Fas-induced apoptosis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Schlegel J,Peters I,Orrenius Sdoi
10.1016/0014-5793(95)00374-isubject
Has Abstractpub_date
1995-05-08 00:00:00pages
139-42issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(95)00374-Ijournal_volume
364pub_type
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