Abstract:
:Measurement of the weight of desmosterol produced during its biosynthesis in the presence of tritiated water and triparanol has permitted a direct determination of the relative flux of carbon and tritium (the H/C ratio) into sterol in hepatocytes. The H/C ratio increased with time of incubation irrespective of the nutritional state of the donor animals. This increase was more marked in hepatocytes from starved animals. Pyruvate and lactate increased, and glucagon decreased, the sterol H/C ratio. Addition of pyruvate to incubations containing glucagon resulted in a 32-67% increase in the H/C ratio depending upon nutritional status. Insulin had no effect whilst (-)-hydroxycitrate decreased the ratio by 25%.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Björnsson OG,Pullinger CR,Gibbons GFdoi
10.1016/0014-5793(85)81264-3subject
Has Abstractpub_date
1985-08-05 00:00:00pages
302-6issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(85)81264-3journal_volume
187pub_type
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