Abstract:
:Major myelin proteolipid (MMPL, also called PLP) and DM-20 are the two major intrinsic membrane proteins of CNS myelin. A specific immunological probe was obtained for MMPL by raising antibodies against the synthetic tridecapeptide 117-129 of MMPL. Antibodies against this peptide reacted with the MMPL but did not cross react with DM-20, while both proteolipids had been shown previously to be recognized by antibodies directed against the C-terminal hexapeptide of MMPL. This is in accordance with previous findings showing that DM-20 differs only from MMPL by a deletion of residues 100-140 (+/- few units). Furthermore, this site-specific immunological probe also recognizes MMPL in its native form in oligodendrocytes in primary glial cell cultures.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Trifilieff E,Luu B,Nussbaum JL,Roussel G,Espinosa de los Monteros A,Sabatier JM,Van Rietschoten Jdoi
10.1016/0014-5793(86)80412-4subject
Has Abstractpub_date
1986-03-31 00:00:00pages
235-9issue
2eissn
0014-5793issn
1873-3468journal_volume
198pub_type
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