Abstract:
:The assembly of cytochrome c oxidase subunits I-III was studied in vitro in isolated rat liver mitochondria pre-labeled with [35S]methionine. Individual subunits were immunoabsorbed with monospecific antibodies. Isolated heme a from rat liver mitochondria, when added to radiolabeled mitochondria, induced assembly of subunit I with subunits II and III. Assembly of these subunits was not observed in mitochondria incubated in the presence of heme b(hemin) or in the absence of heme. Quantitative analysis of immunoabsorbed, radiolabeled subunits suggests that the predominant effect of heme a is on the assembly of subunit I with subunit III.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Wielburski A,Nelson BDdoi
10.1016/0014-5793(84)81302-2subject
Has Abstractpub_date
1984-11-19 00:00:00pages
291-4issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(84)81302-2journal_volume
177pub_type
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