Molecular identification of the enzyme responsible for the mitochondrial NADH-supported ammonium-dependent hydrogen peroxide production.

abstract：:Human D-type prostanoid (DP) and E-type prostanoid 2 (EP2) receptors are regarded as the most closely related receptors among prostanoid receptors. Although these receptors are generated by tandem duplication, their physiological outputs often oppose one another. In the present study, we extracted mutations occurring ...

journal_title：FEBS letters

authors： Tanimoto J,Fujino H,Takahashi H,Murayama T

更新日期：2015-03-12 00:00:00

abstract：:Some pathogenic mutations associated with Alzheimer's disease are thought to affect structural-dynamic properties and the lateral dimerization of amyloid precursor protein (APP) in neuron membrane. Dimeric structure of APP transmembrane fragment Gln(686)-Lys(726) was determined in membrane-mimicking dodecylphosphochol...

journal_title：FEBS letters

authors： Nadezhdin KD,Bocharova OV,Bocharov EV,Arseniev AS

更新日期：2012-06-12 00:00:00

abstract：:In the past two decades, the ATP-binding cassette (ABC) transporters' field has undergone a structural revolution. The importance of structural biology to the development of the field of ABC transporters cannot be overstated, as the ensemble of structures not only revealed the architecture of ABC transporters but also...

journal_title：FEBS letters

authors： Lewinson O,Orelle C,Seeger MA

更新日期：2020-10-24 00:00:00

abstract：:Na+ transport through the F0 domain of Na(+)-F1F0-ATPases involves the combined action of subunits c and a but the residues involved in Na+ liganding in subunit a are unknown. As a first step towards the identification of these residues, we have cloned and sequenced the gene encoding subunit a of the Na(+)-F1F0-ATPase...

journal_title：FEBS letters

authors： Rahlfs S,Müller V

更新日期：1999-06-18 00:00:00

abstract：:The cellulases cellobiohydrolase 1 (CBH 1) and endoglucanase 1 (EG 1) from the fungus Trichoderma reesei are closely related with 40% sequence identity and very similar in structure. In CBH 1 the active site is enclosed by long loops and some antiparallel beta-strands forming a 40 A long tunnel, whereas in EG 1 part o...

journal_title：FEBS letters

authors： Henriksson H,Ståhlberg J,Isaksson R,Pettersson G

更新日期：1996-07-29 00:00:00

abstract：:The mechanisms controlling fat depot-specific metabolism are poorly understood. During starvation of mice, downregulation of lipogenic genes, suppression of fatty acid synthesis, and increases in lipid oxidation were all more pronounced in epididymal than in subcutaneous fat. In epididymal fat, relatively strong upreg...

journal_title：FEBS letters

authors： Sponarova J,Mustard KJ,Horakova O,Flachs P,Rossmeisl M,Brauner P,Bardova K,Thomason-Hughes M,Braunerova R,Janovska P,Hardie DG,Kopecky J

更新日期：2005-11-07 00:00:00

abstract：:In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain...

journal_title：FEBS letters

authors： Kuwayama H,Van Haastert PJ

更新日期：1998-03-13 00:00:00

abstract：:A human vesicular monoamine transporter cDNA has been identified by screening a human brainstem library using sequences from the rat brain synaptic vesicle monoamine transporter (SVMT) [(1992) Cell 70, 539-551; (1992) Proc. Natl. Acad. Sci. USA 89, 10993-10997]. The hSVMT shares 92% amino acid identity with the rat se...

journal_title：FEBS letters

authors： Surratt CK,Persico AM,Yang XD,Edgar SR,Bird GS,Hawkins AL,Griffin CA,Li X,Jabs EW,Uhl GR

更新日期：1993-03-08 00:00:00

abstract：:The efficiency of visible light in inducing fluorescence quenching in the isolated light-harvesting complex II (LHCII) of higher plants is investigated by action spectroscopy in the visible portion of photosynthetic active radiation. The efficiency spectrum displays a relatively homogenous quenching yield across the m...

journal_title：FEBS letters

authors： Santabarbara S,Zubik M,Remelli W,Zucchelli G,Gruszecki WI

更新日期：2016-08-01 00:00:00

abstract：:Recombinant vaccinia virus vectors were used to coexpress each of the candidate prohormone convertases PC1, PC2, furin, PACE4 and PC5 with rat prosomatostatin (rProSOM) in the constitutive secreting cell line LoVo and in the endocrine corticotroph cell line AtT-20, which exhibits regulated secretion. Mammalian ProSOM ...

journal_title：FEBS letters

authors： Brakch N,Galanopoulou AS,Patel YC,Boileau G,Seidah NG

更新日期：1995-04-03 00:00:00

abstract：:The ATP-binding cassette (ABC) transporters constitute one of the largest families of proteins in evolution. The ATM1 gene of the yeast Saccharomyces cerevisiae encodes an ABC protein, which is localized to the mitochondrial inner membrane. A deletion of ATM1 results in the accumulation of up to a 30-fold excess of mi...

journal_title：FEBS letters

authors： Chloupková M,Reaves SK,LeBard LM,Koeller DM

更新日期：2004-07-02 00:00:00

abstract：:Previously determined protein structures have been analysed, in order to find folding motifs similar to that proposed by NMR spectroscopy, for the N-terminal, 14 kDa fragment of the Ada protein. The analyses reveal only limited similarities with the NMR-derived structural data and strongly suggest that this region of ...

journal_title：FEBS letters

authors： Swindells MB

更新日期：1993-06-01 00:00:00

abstract：:Angiotensin II (Ang II), a potent hypertrophic factor for vascular smooth muscle cells (VSMC), induces activation of the ras proto-oncogene product (Ras) and mitogen-activated protein (MAP) kinases, and tyrosine phosphorylation of a focal adhesion-associated protein, paxillin. Forskolin, a direct activator of adenylat...

journal_title：FEBS letters

authors： Takahashi T,Kawahara Y,Okuda M,Yokoyama M

更新日期：1996-11-11 00:00:00

abstract：:Polystyrene microtitre wells are commonly used as supports for the enzyme-linked immunosorbent assay (ELISA) method of biomolecular detection, which is employed in the routine diagnosis of a variety of medical conditions. We have used an atomic force microscope (AFM) to directly monitor specific molecular interactions...

journal_title：FEBS letters

authors： Allen S,Davies J,Dawkes AC,Davies MC,Edwards JC,Parker MC,Roberts CJ,Sefton J,Tendler SJ,Williams PM

更新日期：1996-07-22 00:00:00

abstract：:Monoclonal antibodies (mAbs) against cell surface antigens and receptors are instrumental in defining specific membrane markers. mAbs GF 26.7.3 and MF 25.1 against human neutrophils modulated the activation mechanism of superoxide anion production induced by formyl-peptide and PMA in all subject. However, treatment wi...

journal_title：FEBS letters

authors： Traniello S,Spisani S,Gavioli R,Dovigo L,Baricordi RO,Sensi A,Damiani G

更新日期：1986-08-11 00:00:00

abstract：:We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences...

journal_title：FEBS letters

authors： Bensaid M,Kaghad M,Rodriguez M,Le Fur G,Caput D

更新日期：1993-03-08 00:00:00

abstract：:Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...

journal_title：FEBS letters

authors： Mohr E,Hillers M,Ivell R,Haulica ID,Richter D

更新日期：1985-11-25 00:00:00

abstract：:Activated B61.SF.1 and CTLL-2 T lymphocyte clones which are strictly dependent on interleukin-2 (IL-2) for growth were used to study the activation of Na+/K+-ATPase. 50% of [3H]thymidine maximal incorporation was obtained when the extracellular concentration of Na+ or K+ was reduced to 50 or 2 mM, respectively. 'Quies...

journal_title：FEBS letters

authors： Redondo JM,López Rivas A,Fresno M

更新日期：1986-10-06 00:00:00

abstract：:Lipid droplets (LDs) were perceived as static storage deposits, which passively participate in the energy homeostasis of both cells and entire organisms. However, this view has changed recently after the realization of a complex and highly dynamic LD proteome. The proteome contains key components of the fat mobilizati...

journal_title：FEBS letters

authors： Beller M,Thiel K,Thul PJ,Jäckle H

更新日期：2010-06-03 00:00:00

abstract：:SecM in Escherichia coli has two functionally crucial regions. The arrest motif near the C-terminus interacts with the ribosomal exit tunnel to arrest its own translational elongation. The signal sequence at the N-terminus directs the SecM nascent polypeptide to the Sec-mediated export pathway to release the arrested ...

journal_title：FEBS letters

authors： Nakamori K,Chiba S,Ito K

更新日期：2014-08-25 00:00:00

abstract：:In fat-cells, insulin increases synergistically the inhibitory effects of adenosine and prostaglandin E2 on adenylyl cyclase activity. When the endogenous production of these feedback inhibitors is suppressed, insulin is no more active in conditions where glycosyl inositol-phosphate which is a putative mediator of its...

journal_title：FEBS letters

authors： Martiny L,Dib K,Haye B,Corrèze C,Jacquemin C,Lambert B

更新日期：1991-07-29 00:00:00

abstract：:Pyrroloquinoline quinone (PQQ) has been claimed to be a component of glutamate decarboxylase from Escherichia coli on the basis of a frequently used procedure in which the protein is extracted with hexanol. We demonstrate that if pyridoxal phosphate (PLP) is not added during the preparation, the apoenzyme prepared fro...

journal_title：FEBS letters

authors： De Biase D,Maras B,John RA

更新日期：1991-01-14 00:00:00

abstract：:We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of ...

journal_title：FEBS letters

authors： Macieira-Coelho A

更新日期：1995-01-23 00:00:00

abstract：:The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carbox...

journal_title：FEBS letters

authors： Qin BY,Creamer LK,Baker EN,Jameson GB

更新日期：1998-11-06 00:00:00

abstract：:'Peripheral' binding sites for benzodiazepines are under neural or homonal control in the pineal gland, olfactory bulb, and kidney. These observations prompted a search for an endogenous substance which could modulate these sites under physiological conditions. Acidified methanol extracts from several tissues (e.g. st...

journal_title：FEBS letters

authors： Mantione CR,Weissman BA,Goldman ME,Paul SM,Skolnick P

更新日期：1984-10-15 00:00:00

abstract：:The interactions of DNA duplexes with 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), a potent mutacarcinogen isolated from tryptophan pyrolysate, have been studied using CD spectroscopy. The results are that (a) the spectral change of B-form DNA caused by the interaction with Trp-P-1 is biphasic, i.e. the enla...

journal_title：FEBS letters

authors： Inohara T,Tarui M,Doi M,Inoue M,Ishida T

更新日期：1993-06-21 00:00:00

abstract：:The uncoupling protein-3 (UCP3) is a mitochondrial protein expressed mainly in skeletal muscle. Among several hypotheses for its physiological function, UCP3 has been proposed to prevent excessive production of reactive oxygen species. In the present study, we evaluated the effect of an oxidative stress induced by hyp...

journal_title：FEBS letters

authors： Flandin P,Donati Y,Barazzone-Argiroffo C,Muzzin P

更新日期：2005-06-20 00:00:00

abstract：:Gene duplication provides genetic material required for functional diversification. An interesting example is the amyloid precursor protein (APP) protein family. The APP gene family has experienced both expansion and contraction during evolution. The three mammalian members have been studied quite extensively in combi...

journal_title：FEBS letters

authors： Shariati SA,De Strooper B

更新日期：2013-06-27 00:00:00

abstract：:TATA-box binding factor TFIID is one of the key factors in transcriptional activation. Surprisingly, the yeast TFDII protein [(1989) Nature 341, 299-303; (1989) Cell 56, 1173-1181; (1989) Proc. Natl. Acad. Sci USA 86, 7785-7789] reveals only limited homology with other DNA-binding proteins. From computer-assisted sear...

journal_title：FEBS letters

authors： Stucka R,Feldmann H

更新日期：1990-02-26 00:00:00

abstract：:To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

journal_title：FEBS letters

authors： Wild N,Herberg FW,Hofmann F,Dostmann WR

更新日期：1995-11-06 00:00:00