Abstract:
:Some pathogenic mutations associated with Alzheimer's disease are thought to affect structural-dynamic properties and the lateral dimerization of amyloid precursor protein (APP) in neuron membrane. Dimeric structure of APP transmembrane fragment Gln(686)-Lys(726) was determined in membrane-mimicking dodecylphosphocholine micelles using high-resolution NMR spectroscopy. The APP membrane-spanning α-helix Lys(699)-Lys(724) self-associates in a left-handed parallel dimer through extended heptad repeat motif I(702)X(3)M(706)X(2)G(709)X(3)A(713)X(2)I(716)X(3)I(720)X(2)I(723), whereas the juxtamembrane region Gln(686)-Val(695) constitutes the nascent helix, also sensing the dimerization. The dimerization mechanism of APP transmembrane domain has been described at atomic resolution for the first time and is important for understanding molecular events of APP sequential proteolytical cleavage resulting in amyloid-β peptide.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nadezhdin KD,Bocharova OV,Bocharov EV,Arseniev ASdoi
10.1016/j.febslet.2012.04.062subject
Has Abstractpub_date
2012-06-12 00:00:00pages
1687-92issue
12eissn
0014-5793issn
1873-3468pii
S0014-5793(12)00369-9journal_volume
586pub_type
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