Binding of a de novo designed peptide to specific glycosaminoglycans.

Abstract:

:The binding of glycosaminoglycans to a synthetic peptide (SKAQKAQAKQAKQAQKAQKAQAKQAKQW-CONH(2)), consisting of a hybrid consensus heparin binding sequence, is studied using circular dichroism, fluorescence anisotropy and nuclear magnetic resonance techniques. The results unveil certain novel features, most importantly, the peptide binds preferentially to iduronic acid containing glycosaminoglycans and the dissociation constant for the peptide-heparin complex was found to be 30 nM. Interestingly, higher order intermolecular association(s)/aggregation was not observed, especially at saturating concentrations of the ligand. The helical structure of the peptide backbone, induced upon binding to a particular glycosaminoglycan is directly related to their binding affinity. In our opinion, studies on such unconventional hybrid peptide sequences containing low density basic amino acid residues would lead to the design of sequence specific glycosaminoglycan binding peptides.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Jayaraman G,Wu CW,Liu YJ,Chien KY,Fang JC,Lyu PC

doi

10.1016/s0014-5793(00)01964-5

keywords:

subject

Has Abstract

pub_date

2000-09-29 00:00:00

pages

154-8

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(00)01964-5

journal_volume

482

pub_type

杂志文章
  • Recognizing the pleckstrin homology domain fold in mammalian phospholipase D using hidden Markov models.

    abstract::Phospholipase D was first described in plant tissue but has recently been shown to occur in mammalian cells where it is activated by cell surface receptors. Its mode of activation by receptors in unclear. Biochemical studies suggest that it may occur downstream of other effector proteins and that small GTP-dependent r...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)00366-x

    authors: Holbrook PG,Geetha V,Beaven MA,Munson PJ

    更新日期:1999-04-09 00:00:00

  • Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG.

    abstract::(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O(2) levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2012.07.065

    authors: Tsukamoto S,Yamashita S,Kim YH,Kumazoe M,Huang Y,Yamada K,Tachibana H

    更新日期:2012-09-21 00:00:00

  • Functional interrogation of kinases and other nucleotide-binding proteins.

    abstract::The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2013.05.008

    authors: Rosenblum JS,Nomanbhoy TK,Kozarich JW

    更新日期:2013-06-27 00:00:00

  • A product of phosphatidylinositol-3 kinase is elevated in dividing HT29 colonic epithelial cells.

    abstract::Phosphatidylinositol 3 kinase (PI-3 kinase) activity has been linked to cell proliferation and growth regulation. Therefore, we studied changes in phosphoinositide metabolism during the cell cycle of HT-29 cells, a colonic epithelial cell line. HT29 cells were treated with the microtubule disrupter, nocodozole, separa...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(93)81381-9

    authors: Dobos GJ,Wu XR,Traynor-Kaplan A

    更新日期:1993-06-14 00:00:00

  • G-proteins in Torpedo marmorata electric organ. Differential distribution in pre- and post-synaptic membranes and synaptic vesicles.

    abstract::The nature of the G-proteins present in the pre- and post-synaptic plasma membranes and in the synaptic vesicles of cholinergic nerve terminals purified from the Torpedo electric organ was investigated. In pre- and post-synaptic plasma membranes, Bordetella pertussis toxin, known to catalyze the ADP-ribosylation of th...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)80190-4

    authors: Toutant M,Bockaert J,Homburger V,Rouot B

    更新日期:1987-09-28 00:00:00

  • Time-dependent regulation of muscle caveolin activation and insulin signalling in response to high-fat diet.

    abstract::We studied the effect of high-fat diet on the expression and activation of the three caveolins in rat skeletal muscle and their association with the insulin signalling cascade. Initial response was characterized by increased signalling through Cav-1 and Cav-3 phosphorylation, suggesting that both participate in an ini...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2009.09.016

    authors: Gómez-Ruiz A,de Miguel C,Campión J,Martínez JA,Milagro FI

    更新日期:2009-10-06 00:00:00

  • Requirements for a conservative protein translocation pathway in chloroplasts.

    abstract::The chloroplast inner envelope translocon subunit Tic110 is imported via a soluble stromal translocation intermediate. In this study an in-organellar import system is established which allows for an accumulation of this intermediate in order to analyze its requirements for reexport. All results demonstrate that the re...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.05.004

    authors: Vojta L,Soll J,Bölter B

    更新日期:2007-06-12 00:00:00

  • Thr90 is a key residue of the bacteriorhodopsin proton pumping mechanism.

    abstract::Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered lambda(max) of 555 nm and pK(a) of As...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03080-0

    authors: Perálvarez A,Barnadas R,Sabés M,Querol E,Padrós E

    更新日期:2001-11-23 00:00:00

  • Inhibition of human telomerase by oligonucleotide chimeras, composed of an antisense moiety and a chemically modified homo-oligonucleotide.

    abstract::Most tumor cells attain their immortality by reactivating telomerase. We report here the telomerase inhibitory potential of chimeric oligonucleotides composed of a 13mer antisense sequence targeting the telomerase RNA template region and a (s4dU)n moiety at its 3' or 5'-end. The increase of the thiolated chain length ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2005.01.041

    authors: Tarkanyi I,Horváth A,Szatmari I,Eizert H,Vámosi G,Damjanovich S,Ségal-Bendirdjian E,Aradi J

    更新日期:2005-02-28 00:00:00

  • Degradation by the 2',5'-phosphodiesterase activity of mouse cells requires the presence of a ribo hydroxyl group in the penultimate position of the oligonucleotide substrate.

    abstract::A series of 9-beta-D-xylofuranosyladenine (xyloA or xyloadenosine) substituted analogs of 2-5A core trimer and tetramer were examined for their ability to be degraded by the 2',5'-phosphodiesterase activity of cytoplasmic extracts of mouse L cells. Two distinct groups of xyloA-substituted analogs could be readily disc...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(87)81358-3

    authors: Torrence PF,Alster D,Huss S,Gosselin G,Imbach JL

    更新日期:1987-02-23 00:00:00

  • Calcium-induced calcium release mediated by a voltage-activated cation channel in vacuolar vesicles from red beet.

    abstract::Little is known about the mechanisms underlying calcium-induced Ca2+ release (CICR) in plants. The slow-activating vacuolar (SV) channel is both permeable to, and activated by Ca2+, and is therefore a prime candidate for a role in CICR. Cytosol-side-out vacuolar membrane vesicles loaded with 45Ca2+ showed voltage- and...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(99)01109-6

    authors: Bewell MA,Maathuis FJ,Allen GJ,Sanders D

    更新日期:1999-09-10 00:00:00

  • Growth factor receptors in helminth parasites: signalling and host-parasite relationships.

    abstract::Parasitic helminths remain major pathogens of both humans and animals throughout the world. The success of helminth infections depends on the capacity of the parasite to counteract host immune responses but also to exploit host-derived signal molecules for its development. Recent progress has been made in the characte...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2006.03.046

    authors: Dissous C,Khayath N,Vicogne J,Capron M

    更新日期:2006-05-22 00:00:00

  • Cloning and chromosomal assignment of a widely expressed human receptor-like protein-tyrosine phosphatase.

    abstract::Insight into the regulation of the actions of the protein-tyrosine kinases will be greatly facilitated by the full characterization of the family of protein-tyrosine phosphatases. A search for novel phosphatases resulted in the isolation of a cDNA, termed HLPR, encoding a member of the family of human receptor-like pr...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)81094-5

    authors: Jirik FR,Janzen NM,Melhado IG,Harder KW

    更新日期:1990-10-29 00:00:00

  • Identification of the axial heme ligands of cytochrome b556 in succinate: ubiquinone oxidoreductase from Escherichia coli.

    abstract::Electron paramagnetic resonance (EPR) and near-infrared magnetic circular dichroism (MCD) have been used to identify the ligands to the cytochrome b556 component of succinate: ubiquinone oxidoreductase (succinate dehydrogenase) from Escherichia coli. The 'highly axial low spin' (HALS) EPR spectrum suggests bis(histidi...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(94)01189-3

    authors: Peterson J,Vibat C,Gennis RB

    更新日期:1994-11-28 00:00:00

  • The non-ionic detergent Brij 58P mimics chaperone effects.

    abstract::The non-ionic detergent Brij 58P is recommended as a stabilizing agent for protein storage; for example, the aggregation-prone chaperone DnaJ can be maintained in solution by low concentrations of Brij 58P. During protein folding studies with alpha-glucosidase, rhodanese and citrate synthase as model proteins, we disc...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(02)03689-x

    authors: Krause M,Rudolph R,Schwarz E

    更新日期:2002-12-04 00:00:00

  • Crystal structure of a single-chain trimer of human adiponectin globular domain.

    abstract::Adiponectin is increasingly recognized as a potential therapeutic agent for the treatment of diabetes and other metabolic diseases. It circulates in plasma as homotrimers and higher-order oliogomers of homotrimers. To facilitate the production of active recombinant adiponectin as a therapeutic tool, we designed a sing...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2012.02.024

    authors: Min X,Lemon B,Tang J,Liu Q,Zhang R,Walker N,Li Y,Wang Z

    更新日期:2012-03-23 00:00:00

  • Suppressors of RNA silencing encoded by plant viruses and their role in viral infections.

    abstract::RNA silencing as a robust host defense mechanism against plant viruses is generally countered by virus-encoded silencing suppressors. This strategy is now increasingly recognized to be used by animal viruses as well. We present here an overview of the common features shared by some of the better studied plant viral si...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审

    doi:10.1016/j.febslet.2005.08.041

    authors: Qu F,Morris TJ

    更新日期:2005-10-31 00:00:00

  • Determination and cellular localization of adenylyl cyclase isozymes expressed in embryonic chick heart.

    abstract::Mammalian heart has been reported to express AC isozymes (types V and VI) that are inhibited by < microM [Ca2+]; avian heart has been reported to express adenylyl cyclase activity that is inhibited by < microM [Ca2]. We have used reverse transcription polymerase chain reaction (RT-PCR) to determine that type V and VI ...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(95)01084-r

    authors: Yu HJ,Unnerstall JR,Green RD

    更新日期:1995-10-23 00:00:00

  • Studies on the synthesis and secretion of transthyretin by the human hepatoma cell line Hep G2.

    abstract::Transthyretin (TTR) is a circulatory protein which plays an important role in the transport of both thyroid hormone and retinol. Hep G2 cells, a human hepatoma-derived cell line, have been used extensively in studies of protein secretion by liver cells. The original description of this cell line indicated that this li...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80049-9

    authors: Blaner WS,Bonifácio MJ,Feldman HD,Piantedosi R,Saraiva MJ

    更新日期:1991-08-05 00:00:00

  • Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-β-glucosidases.

    abstract::Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1002/1873-3468.12059

    authors: Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SG

    更新日期:2016-02-01 00:00:00

  • The induction of adipose conversion in 3T3-L1 cells is associated with early phosphorylation of a 60-kDa nuclear protein.

    abstract::The induction of adipose conversion in 3T3-L1 cells by bezafibrate has been shown to be enhanced by dibutyryl-cAMP. We here report that the induction of adipose conversion in 3T3-L1 cells, by either bezafibrate and dibutyryl cAMP, or isobutylmethyxanthine alone, is associated with a very early phosphorylation of a 60-...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(91)80725-i

    authors: Brandes R,Arad R,Bar-Tana J

    更新日期:1991-07-08 00:00:00

  • Epigallocatechin-3-gallate protects motor neurons and regulates glutamate level.

    abstract::Epigallocatechin-3-gallate (EGCG) is a major component of green tea polyphenols which displays potential properties of anticancer and neuroprotection. Here we show that protection of motor neuron by EGCG is associated with regulating glutamate level in organotypic culture of rat spinal cord. In this model, EGCG blocke...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2010.05.011

    authors: Yu J,Jia Y,Guo Y,Chang G,Duan W,Sun M,Li B,Li C

    更新日期:2010-07-02 00:00:00

  • The sequence of the hemoregulatory peptide is present in Gi alpha proteins.

    abstract::The hemoregulatory peptide PyroGlu-Glu-Asp-Cys-Lys (HP5b), which inhibits myelopoietic colony formation in vitro, is shown to be a sequence motif which is also part of the effector domain of Gi alpha proteins. Out of 8 synthetic peptides with sequence variations of HP5b, those with the closest similarity to the Gi alp...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)81106-x

    authors: Laerum OD,Frostad S,Tøn HI,Kamp D

    更新日期:1990-08-20 00:00:00

  • Genetics and evolution of ultraviolet vision in vertebrates.

    abstract::Various vertebrates use ultraviolet (UV) vision for such basic behaviors as mating, foraging, and predation. We have successfully interchanged the color-sensitivities of the mouse UV pigment and the human blue pigment by introducing forward and reverse mutations at five sites. This unveils for the first time the gener...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)02269-9

    authors: Yokoyama S,Shi Y

    更新日期:2000-12-08 00:00:00

  • The Saccharomyces cerevisiae DNA damage checkpoint is required for efficient repair of double strand breaks by non-homologous end joining.

    abstract::In this work we report that the Saccharomyces cerevisiae RAD9, RAD24, RAD17, MEC1, MEC3 and RAD53 checkpoint genes are required for efficient non-homologous end joining (NHEJ). RAD9 and RAD24 function additionally in this process. Defective NHEJ in rad9Delta-rad24Delta, but not yku80Delta cells, is only partially resc...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(00)01180-7

    authors: de la Torre-Ruiz M,Lowndes NF

    更新日期:2000-02-11 00:00:00

  • Functional and molecular characterization of a peptide transporter in the rat PC12 neuroendocrine cell line.

    abstract::We have studied functional properties of peptide transport in the pheochromocytoma neuroendocrine cell line from rat. The neutral peptide D-Phe-L-Ala (resistant to hydrolysis) is a good substrate for uptake into these cells. Transport is substantially inhibited by diethylpyrocarbonate pretreatment and is stimulated by...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)03081-2

    authors: Hussain I,Zanic-Grubisic T,Kudo Y,Boyd CA

    更新日期:2001-11-23 00:00:00

  • Function of nucleophosmin/B23, a nucleolar acidic protein, as a histone chaperone.

    abstract::We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/s0014-5793(01)02939-8

    authors: Okuwaki M,Matsumoto K,Tsujimoto M,Nagata K

    更新日期:2001-10-12 00:00:00

  • Organization of rat uricase chromosomal gene differs greatly from that of the corresponding plant gene.

    abstract::The rat chromosomal gene for uricase was cloned. The gene spans more than 20 kilobases and the coding region is divided into 8 exons by 7 introns. The organization of the rat uricase gene is so greatly different from that of the soybean gene that the difference may not have been caused only by the removal of some ance...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/0014-5793(90)80789-l

    authors: Motojima K,Goto S

    更新日期:1990-05-07 00:00:00

  • Contribution of IL-12R mediated feedback loop to Th1 cell differentiation.

    abstract::T helper 1 (Th1) cell fate is induced by overlapping signaling pathways, whose kinetic principles and regulatory motifs are largely unknown. We identified a simple positive feedback loop in the STAT4 signaling pathway, whereby activation by IL-12 leads to the increased expression in IL-12 receptor. A computational ana...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.10.007

    authors: Becskei A,Grusby MJ

    更新日期:2007-11-13 00:00:00

  • Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium.

    abstract::The white-rot fungus Phanerochaete chrysosporium has two intracellular beta-glucosidases (BGL1A and BGL1B) belonging to glycoside hydrolase (GH) family 1. BGL1B effectively hydrolyzes cellobiose and cellobionolactone, but BGL1A does not. We have determined the crystal structure of BGL1A in substrate-free and gluconola...

    journal_title:FEBS letters

    pub_type: 杂志文章

    doi:10.1016/j.febslet.2007.03.009

    authors: Nijikken Y,Tsukada T,Igarashi K,Samejima M,Wakagi T,Shoun H,Fushinobu S

    更新日期:2007-04-03 00:00:00