Abstract:
:Annexin-I is a calcium dependent phospholipid binding and phospholipase A2 (PLA2) inhibitory protein. A 'substrate depletion' model has been proposed for the mechanism of PLA2 inhibition by annexin-I in studies with 14 to 18 kDa PLA2s. Herein, we have studied the inhibition mechanism using 100 kDa cytosolic PLA2 from porcine spleen. The inhibition has been measured at various substrate and calcium ion concentrations. The pattern of PLA2 inhibition by annexin-I was consistent with a 'specific interaction' mechanism rather than the 'substrate depletion' model. Apparent contraction with previous studies can be explained by the calcium-dependent binding of annexin-I to the substrate.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kim KM,Kim DK,Park YM,Kim CK,Na DSdoi
10.1016/0014-5793(94)80566-0subject
Has Abstractpub_date
1994-05-02 00:00:00pages
251-5issue
3eissn
0014-5793issn
1873-3468journal_volume
343pub_type
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