An improved capping unit for stabilizing the ends of associated β-strands.

abstract：:Limb girdle muscular dystrophy type 2A is linked to a skeletal muscle-specific calpain isoform known as p94. Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity. We have expressed a C-terminally truncated form ...

journal_title：FEBS letters

authors： Rey MA,Davies PL

更新日期：2002-12-18 00:00:00

abstract：:In freshly prepared low density lipoprotein (LDL), ascorbate inhibited LDL oxidation by macrophages at the higher concentrations tested (60-100 microM). In contrast, with LDL that had been allowed to autoxidise in the refrigerator (3 degrees C) for at least 10 weeks after isolation (mildly oxidised or minimally-modifi...

journal_title：FEBS letters

authors： Stait SE,Leake DS

更新日期：1994-03-21 00:00:00

abstract：:The complete amino acid sequence of human plasma apolipoprotein C-III (apoC-III) isolated from normal subjects is described. ApoC-III is a linear polypeptide chain of 79 amino acids. Tryptic digestion of intact apoC-III produced 5 major peptides, while tryptic digestion of the citraconylated protein yielded two peptid...

journal_title：FEBS letters

authors： Hospattankar AV,Brewer HB Jr,Ronan R,Fairwell T

更新日期：1986-03-03 00:00:00

abstract：:A variant form of the heptacosapeptide amide secretin, with C-terminal -Val-Gly-Lys-Arg instead of valine amide, has been isolated from porcine upper intestinal tissue. Unexpectedly, this triacontapeptide exhibited a substantially higher bioactivity than the heptacosapeptide amide. ...

journal_title：FEBS letters

authors： Gafvelin G,Carlquist M,Mutt V

更新日期：1985-05-20 00:00:00

abstract：:A homogeneous protein with a subunit apparent molecular mass of ∼50 kDa that catalyzes the previously described mitochondrial NADH-supported ammonium-stimulated hydrogen peroxide production (Grivennikova, V.G., Gecchini, G. and Vinogradov, A.D. (2008) FEBS Lett. 583, 1287-1291) was purified from the mitochondrial matr...

journal_title：FEBS letters

authors： Kareyeva AV,Grivennikova VG,Cecchini G,Vinogradov AD

更新日期：2011-01-21 00:00:00

abstract：:A cDNA encoding stromal ascorbate peroxidase (sAPX) was isolated using poly(A)+ RNA of pumpkin cotyledons by RT-PCR. The cDNA encodes a polypeptide with 372 amino acids and shares complete sequence identity with pumpkin thylakoid-bound ascorbate peroxidase (tAPX), except for the deletion of a putative membrane spannin...

journal_title：FEBS letters

authors： Mano S,Yamaguchi K,Hayashi M,Nishimura M

更新日期：1997-08-11 00:00:00

abstract：:The F(1)F(0) ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F(1) and subunit...

journal_title：FEBS letters

authors： Peng G,Bostina M,Radermacher M,Rais I,Karas M,Michel H

更新日期：2006-10-30 00:00:00

abstract：:Previous studies of oxygen-derived free radical generation based on spin-trapping methods have shown a signal formed of six bands (sextet) using electron spin resonance spectrometry (ESR) of coronary effluents collected during post-ischaemic reperfusion of isolated hearts perfused with 5,5-dimethyl-1-pyrroline N-oxide...

journal_title：FEBS letters

authors： Boucher F,Pucheu S,Coudray C,Favier A,de Leiris J

更新日期：1992-05-18 00:00:00

abstract：:Mechanisms underlying the association between fibroblastic growth factor 23 (FGF-23) and inflammation are uncertain. We found that FGF-23 was markedly up-regulated in LPS/INF-γ-induced proinflammatory M1 macrophages and Hyp mouse-derived peritoneal macrophages, but not in IL-4-induced M2 anti-inflammatory macrophages....

journal_title：FEBS letters

authors： Han X,Li L,Yang J,King G,Xiao Z,Quarles LD

更新日期：2016-01-01 00:00:00

abstract：:Protein gene product 9.5 (PGP 9.5), which in the normal nervous system is restricted to certain neurons, has been detected in two glioma cell lines, rat C6 and human GL15, by immunoblotting and immunocytochemistry. Its expression in these cells depends on the cellular growth state, being maximal between the first and ...

journal_title：FEBS letters

authors： Giambanco I,Bianchi R,Ceccarelli P,Pula G,Sorci G,Antonioli S,Bocchini V,Donato R

更新日期：1991-09-23 00:00:00

abstract：:Neurofibrillary tangles formed by abnormally aggregated tau protein are a histopathological feature of tauopathies. A tau aggregation inhibitor is a potential therapeutic agent for tauopathies. In this study, we prepared a monoclonal antibody for tau, monoclonal antibody to tau protein (Tau2r3), using as epitope the 2...

journal_title：FEBS letters

authors： Tsuchida T,Susa K,Kibiki T,Tsuchiya T,Miyamoto K,In Y,Minoura K,Taniguchi T,Ishida T,Tomoo K

更新日期：2020-04-13 00:00:00

abstract：:Prokaryotes, plants and animals control ammonium fluxes by the regulated expression of ammonium transporters (AMTs) and/or the related Rhesus (Rh) proteins. Plant AMTs were previously reported to mediate electrogenic transport. Functional analysis of AtAMT2 from Arabidopsis in yeast and oocytes suggests that NH(4)(+) ...

journal_title：FEBS letters

authors： Neuhäuser B,Dynowski M,Ludewig U

更新日期：2009-09-03 00:00:00

abstract：:Interleukin-8 (neutrophil-activating factor; NAP-1) has been crystallized by the vapour diffusion technique to give single crystals suitable for three-dimensional structural study at a resolution higher than 2.4 A. The crystals belong to the space group P3(1)21 or P3(2)21 and have unit cell dimensions a = b = 40.9 A, ...

journal_title：FEBS letters

authors： Auer M,Kallen J,Schleischitz S,Walkinshaw MD,Wasserbauer E,Ehn G,Lindley IJ

更新日期：1990-06-04 00:00:00

abstract：:Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...

journal_title：FEBS letters

authors： Kittridge KJ,Willson RL

更新日期：1984-05-07 00:00:00

abstract：:ZAP-70 is a protein tyrosine kinase (PTK) that plays a critical role in T cell activation. To study the role of ZAP-70 catalytic activity in this process, a substrate capable of distinguishing between the activities of ZAP-70 and other PTKs would be useful, especially since it has recently been shown that ZAP-70 inter...

journal_title：FEBS letters

authors： Watts JD,Brabb T,Bures EJ,Wange RL,Samelson LE,Aebersold R

更新日期：1996-12-02 00:00:00

abstract：:The nuclear magnetic resonance solution structure of alpha-conotoxin SI has been determined at pH 4.2. The 36 lowest energy structures show that alpha-conotoxin SI exists in a single major solution conformation and is stabilized by six hydrogen bonds. Comparisons are made between the SI solution structure and the solu...

journal_title：FEBS letters

authors： Benie AJ,Whitford D,Hargittai B,Barany G,Janes RW

更新日期：2000-07-07 00:00:00

abstract：:ABCA3 mutations cause fatal surfactant deficiency and interstitial lung disease. ABCA3 protein is a lipid transporter indispensible for surfactant biogenesis and storage in lamellar bodies (LB). The protein folds in endoplasmic reticulum and is glycosylated in Golgi en route to the membrane of mature LB and their prec...

journal_title：FEBS letters

authors： Engelbrecht S,Kaltenborn E,Griese M,Kern S

更新日期：2010-10-22 00:00:00

abstract：:Two genome sequences of Escherichia coli K-12 substrains, one partial W3110 and one complete MG1655, have been determined by Japanese and American genome projects, respectively. In order to estimate the rate of nucleotide changes, we directly compared 2 Mb of the nucleotide sequences from these closely-related E. coli...

journal_title：FEBS letters

authors： Itoh T,Okayama T,Hashimoto H,Takeda J,Davis RW,Mori H,Gojobori T

更新日期：1999-04-30 00:00:00

abstract：:Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

journal_title：FEBS letters

authors： McIntyre GD,Chaudhuri S,Coggins JR

更新日期：1984-10-01 00:00:00

abstract：:The transmembrane protein gp130 is the common signalling receptor subunit for the interleukin-6 (IL-6)-type cytokines. It has recently been shown that the cytoplasmic domain of gp130 contains a dileucine internalization motif and that endocytosis of gp130 occurs signal-independent. Here, we have studied whether gp130 ...

journal_title：FEBS letters

authors： Thiel S,Dahmen H,Martens A,Müller-Newen G,Schaper F,Heinrich PC,Graeve L

更新日期：1998-12-18 00:00:00

abstract：:Complexes between the urokinase-type plasminogen activator (uPA) and its type-2 inhibitor (PAI-2) are bound by a cell-surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa. The 70-kDa fragment contains the active site of uPA and PAI-2, while the 22-kDa species was identified as the amino ter...

journal_title：FEBS letters

authors： Ragno P,Montuori N,Vassalli JD,Rossi G

更新日期：1993-06-01 00:00:00

abstract：:Insertion and deletion analyses of a protein have been less common than point mutation analyses, partly due to the lack in effective methods. This is the case with the green fluorescent protein (GFP), which is so widely applied in molecular biology and other fields. In this paper we first introduce a systematic approa...

journal_title：FEBS letters

authors： Kitamura K,Yoshida C,Nishigaki K

更新日期：2003-12-18 00:00:00

abstract：:The rate of mitochondrial ATPase inactivation by the naturally occurring inhibitor protein in the presence of saturating ATP and Mg2+ at pH 8.0 depends hyperbolically on the amount of inhibitor added; the upper limit of an apparent first-order constant for the inactivation process is 1.0(-1) at 25 degrees C. A dramati...

journal_title：FEBS letters

authors： Panchenko MV,Vinogradov AD

更新日期：1985-05-20 00:00:00

abstract：:The lack of trehalose accumulation in most plant species has been partly attributed to the presence of an active trehalase. Although trehalose synthesis enzymes are thought to be cytosolic, and previous studies have indicated that trehalase activity is extracellular, the exact location of the enzyme has not yet been e...

journal_title：FEBS letters

authors： Frison M,Parrou JL,Guillaumot D,Masquelier D,François J,Chaumont F,Batoko H

更新日期：2007-08-21 00:00:00

abstract：:Familial porphyria cutanea tarda (PCT) is a photocutaneous disease in which subnormal activity of uroporphyrinogen decarboxylase is observed both in the liver and red cells. Hepatic iron plays a key role in the genesis of overt biochemical and clinical PCT. In this report, we have studied the properties of 10 000-fold...

journal_title：FEBS letters

authors： Mukerji SK,Pimstone NR,Tan KT

更新日期：1985-09-23 00:00:00

abstract：:To obtain information on the biological significance of yeast fructose-2,6-bisphosphate 6-phosphatase, kinetic data of the purified enzyme [(1987) Eur. J. Biochem. 164, 27-30] have been measured. Maximal activity was found between pH 6 and 7, the apparent Michaelis constant with fructose 2,6-bisphosphate was 7.2 micro...

journal_title：FEBS letters

authors： Plankert U,Purwin C,Holzer H

更新日期：1988-10-24 00:00:00

abstract：:Apomyoglobin was found to break down phosphatidylcholine vesicles to form a micellar complex at pH 4. Size reduction during this process was observed by measuring light scattering at 400 nm and by passing the complex through a gel-filtration column. Micellar complex formation was confirmed by electron microscopy. ...

journal_title：FEBS letters

authors： Lee JW,Kim H

更新日期：1988-12-05 00:00:00

abstract：:Bovine insulin can be split into several fractions by gel filtration on Sephadex G50. These are, in order of decreasing molecular weight, a new unidentified substance, proinsulin, and a large fraction containing several substances. Ultracentrifugal examination of the proinsulin in acetic acid solution (pH 2.3) gave a ...

journal_title：FEBS letters

authors： Zühlke H,Behlke J

更新日期：1968-12-01 00:00:00

abstract：:Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...

journal_title：FEBS letters

authors： Segond N,Jullienne A,Tahri EH,Garel JM

更新日期：1984-08-20 00:00:00

abstract：:The chemokine RANTES is a potent chemoattractant for eosinophils. RANTES is produced by lung epithelial cells during eosinophil-rich inflammatory diseases such as asthma. In this study, we examined the effects of thiazolidinediones (TZD) on RANTES expression in a human lung epithelial cell line, A549. In A549 cells, i...

journal_title：FEBS letters

authors： Momoi A,Murao K,Imachi H,Sayo Y,Nakamura H,Hosokawa H,Sato M,Fujita J,Okada H,Ishida T,Takahara J

更新日期：1999-06-11 00:00:00