Abstract:
:A cDNA encoding stromal ascorbate peroxidase (sAPX) was isolated using poly(A)+ RNA of pumpkin cotyledons by RT-PCR. The cDNA encodes a polypeptide with 372 amino acids and shares complete sequence identity with pumpkin thylakoid-bound ascorbate peroxidase (tAPX), except for the deletion of a putative membrane spanning region located in the carboxy domain of tAPX. Southern blot hybridization and analysis of intron structure indicated that mRNAs for sAPX and tAPX, whose suborganellar localizations in chloroplasts are different, are produced by alternative splicing. Immunoblot analysis showed that the accumulation of sAPX and tAPX was differently regulated during germination and subsequent greening of pumpkin cotyledons.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mano S,Yamaguchi K,Hayashi M,Nishimura Mdoi
10.1016/s0014-5793(97)00862-4subject
Has Abstractpub_date
1997-08-11 00:00:00pages
21-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(97)00862-4journal_volume
413pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80131-l
更新日期:1991-01-28 00:00:00
abstract::The possible regulation of the expression of uncoupling protein-2 (UCP2) mRNA by thyroid hormones in different tissues was examined in rats. Triiodothyronine (T3) was found to produce an organ-specific enhancement of UCP2 expression in rat tissues. The effect of T3 was markedly observed in heart, whereas a moderate ef...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01375-6
更新日期:1997-11-24 00:00:00
abstract::Incubating either Chinese hamster ovary (CHO) cells or 3T3-L1 adipocytes with insulin increased the phosphorylation of the eIF-4E-binding protein, PHAS-I. Insulin also activated p70S6K and the Erk-1 and Erk-2 isoforms of mitogen-activated protein kinase (MAP kinase). However, the concentrations of the hormone needed t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00500-0
更新日期:1997-06-09 00:00:00
abstract::Lysosomal beta-galactosidase precursor is processed to a mature form and associated with protective protein in lysosomes. In this study we used two cysteine protease proinhibitors, E64-d for cathepsins B, S, H, and L, and CA074Me for cathepsin B. They are converted intracellularly to active forms, E-64c and CA074, res...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01461-0
更新日期:1997-12-15 00:00:00
abstract::The Type I cGMP-dependent protein kinase catalytic domain (residues 336-671 from the I alpha isoform) has been expressed as a cGMP independent kinase in a baculovirus system. Using peptide substrates, the protein retains similar substrate specificity as the native holoenzyme. The recombinant catalytic domain catalyzes...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80512-1
更新日期:1994-04-11 00:00:00
abstract::Canine aortic strip studies revealed insensitivity of angiotensin II (AII)-induced aortic contraction to inhibition by the non-peptide antagonist DuP753 (pKB = 6.7 +/- 0.1). In order to determine the origin of this phenomenon we cloned the canine homologue of the AT1 AII receptor. Expression of this cDNA in COS-7 cell...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)80307-2
更新日期:1994-04-25 00:00:00
abstract::Chemotaxis induction is a major effect evoked by stimulation of the chemokine receptor CXCR4 with its sole ligand CXCL12. We now report that treatment of CHP-100 human neuroepithelioma cells with the glucosylceramide synthase (GCS) inhibitor DL-threo-1-phenyl-2-hexadecanoylamino-3-pyrrolidino-1-propanol inhibits CXCR4...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.05.003
更新日期:2007-06-12 00:00:00
abstract::Eukaryotic nuclear RNA binding proteins share a common sequence motif thought to be implicated in RNA binding. One of the two domains present in A1 hnRNP protein, has been modelled by homology in order to make a prediction of the main features of the RNA binding site. Acylphosphatase (EC 3.6.1.7) was selected as templ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80863-e
更新日期:1990-12-17 00:00:00
abstract::Both soluble and microsomal fractions of Fusarium oxysporum contain cytochrome P-450(P-450). We report here that the P-450 in the soluble fraction was induced only when nitrate or nitrite was added to the growth medium, whereas the microsomal P-450 was synthesized regardless of the medium compositions. The reduced-CO ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81151-2
更新日期:1989-02-13 00:00:00
abstract::Anomalously high values of photoinduced absorption changes were revealed in the antenna of photosynthetic purple bacteria. They were found to be 4-16 times greater at the bleaching peak of the antenna than at the bleaching peak of the BChl dimer of the reaction center. This is direct proof of excitation delocalization...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00663-t
更新日期:1995-07-17 00:00:00
abstract::In the current work, we report that specific inhibition of Janus tyrosine kinase (JAK3) via NC1153 induces apoptosis of certain leukemia/lymphoma cell lines. Affymetrix microarray profiling following NC1153 treatment unveiled JAK3 dependent survival modulating pathways (p53, TGF-beta, TNFR and ER stress) in Kit225 cel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.071
更新日期:2010-04-16 00:00:00
abstract::Phosphorylation of histone variant H2AX at serine 139, named gammaH2AX, has been widely used as a sensitive marker for DNA double-strand breaks (DSBs). gammaH2AX is required for the accumulation of many DNA damage response (DDR) proteins at DSBs. Thus it is believed to be the principal signaling protein involved in DD...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.05.021
更新日期:2010-09-10 00:00:00
abstract::Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.03.006
更新日期:2009-04-02 00:00:00
abstract::Pyoverdine I (PVDI) is the major siderophore produced by Pseudomonas aeruginosa to import iron. Biosynthesis of this chelator involves non-ribosomal peptide synthetases and other enzymes. PvdQ is a periplasmic enzyme from the NTN hydrolase family and is involved in the final steps of PVDI biosynthesis. A pvdQ mutant p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2011.12.004
更新日期:2012-01-02 00:00:00
abstract::Intracellular vesicular transport involves targeting of vesicles to their correct destination and subsequent fusion with the target membrane. These processes employ unique compartment-specific proteins in combination with general components which act at several transport steps. This review will summarize recent progre...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(95)00594-y
更新日期:1995-08-01 00:00:00
abstract::The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81341-1
更新日期:1984-05-21 00:00:00
abstract::Mitochondria process local and global Ca(2+) signals. Thereby the spatiotemporal patterns of mitochondrial Ca(2+) signals determine whether the metabolism of these organelles is adjusted or cell death is executed. Mitochondrial Ca(2+) channels of the inner mitochondrial membrane (IMM) actually implement mitochondrial ...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2010.01.010
更新日期:2010-05-17 00:00:00
abstract::Studies were designed to elucidate the mechanism by which unsaturated fatty acids inhibit the binding of triiodothyronine (T3) ro rat liver nuclei. The possibility of a direct interaction between oleic acid and T3 was excluded by dialysis experiments. Oleic acid inhibits nuclear T3 binding in a strictly competitive ma...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)80242-x
更新日期:1989-03-27 00:00:00
abstract::In this work, we have spectroscopically characterised CYP157C1 from Streptomyces coelicolor A3(2) which has the motif E(297)QSLW(301) rather than the invariant EXXR motif in the P450 K-helix. Site-directed mutagenesis of native E(297)QSLW(301) in CYP157C1 to E(297)ESLR(301) or E(297)QSRW(301) both containing standard ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.043
更新日期:2006-11-27 00:00:00
abstract::ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.09.074
更新日期:2004-11-05 00:00:00
abstract::The early response to bacterial inoculation has been investigated and two Arabidopsis genes, ap3.3a and ap4.3a have been characterized. The AP3.3A protein showed high identity to centrin, a ubiquitous cytoskeletal protein first identified in unicellular green alga. Amino-acid sequence analyses of the AP4.3A protein in...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01015-1
更新日期:1998-09-04 00:00:00
abstract::The fibronectin type II (FnII) domain, present in diverse vertebrate proteins, plays crucial roles in several fundamental biological processes. PDC-109, the major bovine seminal plasma protein, contains two FnII domains that bind to choline phospholipids on sperm plasma membrane and induce lipid efflux crucial for suc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13617
更新日期:2020-02-01 00:00:00
abstract::Mitochondria in primary living hepatocytes were visualized in cells transfected with a chimeric plasmid encoding for the green fluorescent protein (GFP) of Aequorea victoria engineered to be specifically targeted to mitochondria, as described recently (Rizutto et al. (1995) Curr. Biol. 5, 635-642). The identification ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00182-2
更新日期:1996-03-18 00:00:00
abstract::Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Ju...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00190-8
更新日期:1999-03-05 00:00:00
abstract::The aim of the present study is to determine the role of intracellular Ca(2+) in VEGF signaling. We demonstrate that reduction in Ca(2+) by chelating compound BAPTA-AM or by IP(3)-endoplasmic reticulum blocker 2-APB selectively inhibited VEGF-induced activation of c-Src-PI3K-Akt but not ERK1/2 in human coronary artery...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.05.053
更新日期:2010-07-16 00:00:00
abstract::The modifying effects of the products of the equimolar addition Of DL-alanine and DL-alanyl-DL-alanine to fullerene C60 on the structure and permeability of the lipid bilayer of phosphatidylcholine liposomes has been studied using the luminescence probe technique. It is shown that these water soluble amino acid and di...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00537-6
更新日期:1996-07-01 00:00:00
abstract::The effect of synthetic cationic lipids on the structure of DNA was studied. The fluorescence enhancement of ethidium bromide on intercalation into DNA was suppressed by the addition of bilayer-forming lipids, but not by micellar ones. Results on the fluorescence depolarization index suggest that ethidium bromide is n...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00736-3
更新日期:1996-08-05 00:00:00
abstract::Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in lambda gt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a pu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80871-2
更新日期:1987-08-10 00:00:00
abstract::Human Tid-1 (hTid-1) is a DnaJ chaperone protein with homology to the Drosophila tumor suppressor Tid56. We report the first case of a tumor-associated mutation at the human TID1 locus, which was identified in the SF767 glioma cell line giving rise to aberrantly high levels of a hTid-1(L) mutant variant. In this study...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.034
更新日期:2004-12-17 00:00:00
abstract::This study focused on the variations in phosphoinositide metabolism depending upon alphaIIbbeta3-integrin/fibrinogen interaction without previous activation of platelet agonist receptors. We found that adhesion of resting human platelets to immobilized fibrinogen stimulates phosphatidic acid production and a concomita...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00595-9
更新日期:1996-07-08 00:00:00