Abstract:
:Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M ascorbate can substantially protect both LDH and YADH from OH attack, in the presence of 10(-3)M urate only LDH is protected. In the case of YADH an even greater degree of inactivation than with OH occurs. The extent of inactivation is considerably reduced when oxygen is absent, in agreement with a urate peroxy radical perhaps being partly responsible for the increased inactivation of the enzyme.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kittridge KJ,Willson RLdoi
10.1016/0014-5793(84)81391-5subject
Has Abstractpub_date
1984-05-07 00:00:00pages
162-4issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(84)81391-5journal_volume
170pub_type
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