Abstract:
:The residues on MAP kinase kinase-1 (MAPKK1) phosphorylated by MAP kinase in vitro have been identified as Thr-291 and Thr-385. Both threonines are phosphorylated in PC12 cells and the 32P-labelling of each residue increases after stimulation with nerve growth factor (NGF). The results establish that MAPKK1 is a physiological substrate for MAP kinase. The two active forms of MAPKK that are resolved by Mono Q chromatography of PC12 cell extracts are both phosphorylated at Thr-291 and Thr-385, demonstrating that neither species is the MAPKK2 isoform which lacks Thr-291.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Saito Y,Gomez N,Campbell DG,Ashworth A,Marshall CJ,Cohen Pdoi
10.1016/0014-5793(94)80252-1subject
Has Abstractpub_date
1994-03-14 00:00:00pages
119-24issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(94)80252-1journal_volume
341pub_type
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